7VIH: Gi coupled Sphingosine 1-phosphate receptor

Cryo-EM structure of Gi coupled Sphingosine 1-phosphate receptor bound with CBP-307. Determined by electron microscopy at 2.98 Å resolution. Released 28 Sept 2022.

Method: Electron microscopy
Resolution: 2.98 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,938
Mol. weight: 159.14 kDa
Ligands: 7I4
Released: 28 Sept 2022

Explore 7VIH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7VIH contains 33 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-23	21
α-helix	29-32	4
α-helix	37-38	2
β-strand	46-50	5	1
β-strand	57-61	5	2
β-strand	68-73	6	2
β-strand	77-82	6	2
β-strand	87-93	7	2
β-strand	99-104	6	3
β-strand	110-115	6	3
β-strand	119-124	6	3
β-strand	133-139	7	3
β-strand	145-150	6	4
β-strand	155-160	6	4
β-strand	165-169	5	4
β-strand	174-179	6	4
β-strand	186-191	6	5
β-strand	197-202	6	5
β-strand	207-211	5	5
β-strand	217-221	5	5
β-strand	228-233	6	6
β-strand	239-244	6	6
β-strand	249-253	5	6
β-strand	259-263	5	6
α-helix	271	1
β-strand	272-277	6	7
β-strand	283-288	6	7
β-strand	293-297	5	7
β-strand	303-306	4	7
β-strand	314-319	6	1
β-strand	326-330	5	1
β-strand	335-338	4	1

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-22	16
α-helix	30-43	14
α-helix	54-55	2

Chain D: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-40	8	16
α-helix	46-55	10
β-strand	185-191	7	16
β-strand	194-200	7	16
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	16
α-helix	227-231	5
α-helix	242-254	13
α-helix	262	1
β-strand	263-269	7	16
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-322	4	16
α-helix	331-351	21

Chain E: 5 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	8
β-strand	11-12	2	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	33-39	7	10
β-strand	45-51	7	10
α-helix	53-55	3
β-strand	58-60	3	10
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	88-90	3
β-strand	92-99	8	10
β-strand	110-111	2	10
β-strand	115-117	3	10
β-strand	118-119	2	9
β-strand	140-141	2	11
β-strand	146-149	4	12
β-strand	155-161	7	11
β-strand	166	1	13
β-strand	172	1	13
β-strand	174-179	6	12
β-strand	186-190	5	12
β-strand	194-195	2	12
α-helix	196	1
β-strand	203-208	6	11
β-strand	211-216	6	11
α-helix	221-223	3
β-strand	225-231	7	12
β-strand	239	1	12
β-strand	243-247	5	12

Chain F: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	24-32	9
α-helix	49-72	24
α-helix	79-104	26
α-helix	106-109	4
α-helix	114-147	34
α-helix	157-180	24
β-strand	183	1	14
β-strand	191	1	15
β-strand	198	1	14
β-strand	199	1	15
α-helix	200-233	34
α-helix	250-281	32
α-helix	294-313	20
α-helix	316-323	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	A	protein	357	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	E	protein	251	Mus musculus
Sphingosine 1-phosphate receptor 1	F	protein	394	Homo sapiens	P21453 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	D	protein	354	Homo sapiens	P63096 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7VIH_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains A)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (C), FASTA

>7VIH_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (E), FASTA

>7VIH_3 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAA

Sequence of entity 4 (F), FASTA

>7VIH_4 Sphingosine 1-phosphate receptor 1 (chains F)
MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENSIKLTSVVFILICCFII
LENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLR
EGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRLFLLISACWVISLILGGLPIM
GWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKN
ISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDVGCKVKTCDILFRAEYFLVLA
VLNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSH
PQKDEGDNPETIMSSGNVNSSSLEHHHHHHHHHH

Sequence of entity 5 (D), FASTA

>7VIH_5 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains D)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Ligands and cofactors

ID	Name	Formula	Copies
7I4	1-[[2-fluoranyl-4-[5-[4-(2-methylpropyl)phenyl]-1,2,4-oxadiazol-3-yl]phenyl]met…	C23 H24 F N3 O3	1

Primary citation

Structural insights into sphingosine-1-phosphate receptor activation. Yu, L., He, L., Gan, B. et al. Proc Natl Acad Sci U S A (2022) 119:e2117716119-e2117716119. DOI 10.1073/pnas.2117716119 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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