7W59: Human pre-C*-I complex

The cryo-EM structure of human pre-C*-I complex. Determined by electron microscopy at 3.6 Å resolution. Released 22 Jun 2022.

Method: Electron microscopy
Resolution: 3.6 Å
Organisms: Homo sapiens, unidentified adenovirus
Chains: 50
Atoms: 92,315
Mol. weight: 2672.89 kDa
Ligands: IHP, GTP, MG, ZN
Released: 22 Jun 2022

Explore 7W59 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7W59 contains 556 α-helices and 434 β-strands across 45 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains a and h: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	5-12	8
β-strand	17-22	6	17
β-strand	27-35	9	17
β-strand	41-49	9	17
β-strand	55-63	9	17
α-helix	65-67	3
β-strand	68-73	6	17

Chain A: 97 helices, 46 β-strands

Element	Residues	Length	Sheet
α-helix	27-44	18
α-helix	47-49	3
α-helix	63-73	11
α-helix	82-84	3
α-helix	85-90	6
α-helix	96-103	8
α-helix	106-108	3
β-strand	115-121	7	1
β-strand	127-129	3	1
β-strand	134-135	2	2
α-helix	137-157	21
α-helix	163-165	3
α-helix	174-177	4
α-helix	178-182	5
α-helix	193-196	4
β-strand	228-229	2	3
α-helix	232-241	10
α-helix	253-255	3
α-helix	260-268	9
α-helix	310-315	6
α-helix	357-358	2
α-helix	396-403	8
β-strand	415-416	2	3
β-strand	419-420	2	2
α-helix	428-431	4
α-helix	441-459	19
α-helix	473-477	5
β-strand	482-488	7	1
α-helix	489-510	22
β-strand	516-518	3	4
β-strand	524-526	3	4
α-helix	532-536	5
α-helix	542-551	10
α-helix	553-565	13
α-helix	572-583	12
α-helix	585-588	4
α-helix	596-598	3
α-helix	599-616	18
α-helix	631-639	9
α-helix	641-661	21
α-helix	677-689	13
α-helix	706-716	11
α-helix	718-721	4
α-helix	733-752	20
α-helix	754-762	9
α-helix	769-796	28
α-helix	806-820	15
α-helix	822-825	4
α-helix	835-847	13
α-helix	858-872	15
α-helix	874-887	14
β-strand	894-900	7	5
β-strand	905-910	6	5
α-helix	913-932	20
β-strand	940	1	6
α-helix	948-960	13
β-strand	973-980	8	6
α-helix	985-987	3
α-helix	990-998	9
α-helix	1003-1012	10
β-strand	1016-1019	4	5
β-strand	1022-1025	4	5
α-helix	1037-1050	14
α-helix	1055-1062	8
α-helix	1077-1080	4
β-strand	1085-1091	7	6
β-strand	1094-1101	8	6
α-helix	1103-1116	14
α-helix	1140-1141	2
α-helix	1146-1158	13
β-strand	1174-1178	5	6
β-strand	1185-1188	4	7
β-strand	1192	1	8
β-strand	1193-1198	6	7
β-strand	1213-1217	5	7
β-strand	1224-1229	6	7
β-strand	1232	1	8
α-helix	1234-1249	16
α-helix	1256-1264	9
α-helix	1271-1274	4
α-helix	1282-1300	19
α-helix	1308-1310	3
α-helix	1313-1315	3
α-helix	1320-1322	3
β-strand	1334-1337	4	9
α-helix	1340-1346	7
β-strand	1352-1355	4	9
α-helix	1368-1371	4
α-helix	1375-1398	24
α-helix	1409-1411	3
α-helix	1419-1423	5
α-helix	1430-1432	3
α-helix	1437-1442	6
α-helix	1443-1445	3
α-helix	1458-1461	4
α-helix	1467-1469	3
α-helix	1472-1475	4
α-helix	1481-1484	4
α-helix	1485-1487	3
α-helix	1491-1493	3
β-strand	1502	1	10
α-helix	1509-1514	6
α-helix	1520-1527	8
α-helix	1530-1537	8
α-helix	1539-1542	4
β-strand	1552-1554	3	11
β-strand	1561-1563	3	11
α-helix	1567-1576	10
α-helix	1581-1597	17
α-helix	1598-1600	3
β-strand	1606-1611	6	12
β-strand	1629-1637	9	12
β-strand	1643	1	12
β-strand	1657-1667	11	12
α-helix	1677-1687	11
β-strand	1700-1706	7	12
β-strand	1711-1716	6	12
α-helix	1723-1736	14
α-helix	1738-1751	14
β-strand	1754	1	10
α-helix	1768-1772	5
β-strand	1777-1780	4	13
β-strand	1788-1792	5	14
β-strand	1798-1802	5	14
β-strand	1805-1806	2	15
β-strand	1810	1	16
β-strand	1817	1	16
β-strand	1819-1822	4	15
α-helix	1824-1827	4
α-helix	1833-1851	19
β-strand	1860-1863	4	13
α-helix	1866-1868	3
α-helix	1869-1875	7
β-strand	1883-1885	3	13
α-helix	1894-1898	5
α-helix	1900-1908	9
β-strand	1913-1915	3	15
α-helix	1923-1925	3
α-helix	1929-1945	17
α-helix	1949-1954	6
α-helix	1973-1995	23
α-helix	1999-2001	3
α-helix	2004-2011	8

Chain b: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
β-strand	16-21	6	17
β-strand	26-33	8	17
β-strand	40-48	9	17
β-strand	64-72	9	17
α-helix	74-76	3
β-strand	77-84	8	17
α-helix	85-87	3
β-strand	107	1	29
β-strand	112	1	78

Chains c and j: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	3-8	6
β-strand	14-19	6	17
β-strand	24-32	9	17
β-strand	38-46	9	17
β-strand	53-60	8	17
α-helix	62-64	3
β-strand	65-69	5	17
α-helix	70-71	2
α-helix	76-79	4

Chain C: 44 helices, 43 β-strands

Element	Residues	Length	Sheet
α-helix	69-73	5
β-strand	78-81	4	18
α-helix	115-125	11
β-strand	129-136	8	19
α-helix	142-153	12
α-helix	174-177	4
β-strand	184-191	8	19
β-strand	197-204	8	19
α-helix	209-211	3
α-helix	215-221	7
β-strand	224-230	7	19
β-strand	234	1	19
α-helix	237-248	12
β-strand	252-258	7	19
α-helix	260-263	4
α-helix	271-292	22
α-helix	302-304	3
β-strand	307-311	5	19
β-strand	316-318	3	19
α-helix	321-330	10
α-helix	336-340	5
β-strand	348-350	3	20
β-strand	355-357	3	20
α-helix	365-367	3
α-helix	368-369	2
α-helix	370-374	5
α-helix	375-387	13
α-helix	392-398	7
α-helix	407-409	3
α-helix	412-423	12
α-helix	428-436	9
α-helix	441-452	12
β-strand	453	1	21
α-helix	461-465	5
β-strand	475	1	22
β-strand	476-482	7	23
β-strand	491-496	6	23
β-strand	498	1	22
β-strand	501-502	2	24
β-strand	506-510	5	23
α-helix	511	1
β-strand	515	1	25
β-strand	518	1	25
β-strand	524-526	3	23
β-strand	530-534	5	23
β-strand	537-541	5	23
β-strand	543-544	2	24
β-strand	548-552	5	23
β-strand	563-566	4	23
β-strand	577	1	21
α-helix	578-579	2
β-strand	588-594	7	26
α-helix	597-599	3
α-helix	600-613	14
β-strand	618-621	4	26
β-strand	627-631	5	26
α-helix	634-643	10
α-helix	644-648	5
β-strand	653-655	3	26
α-helix	656-658	3
β-strand	659-660	2	26
β-strand	663-666	4	27
β-strand	674-677	4	28
β-strand	684-691	8	28
α-helix	694-701	8
α-helix	711-722	12
α-helix	726-730	5
β-strand	732-734	3	28
β-strand	744-747	4	28
α-helix	750-755	6
α-helix	756-759	4
α-helix	763-773	11
β-strand	784	1	27
β-strand	787-795	9	28
α-helix	808-821	14
β-strand	825-839	15	27
α-helix	840-842	3
α-helix	844-852	9
β-strand	857-862	6	27
α-helix	863	1
β-strand	868-876	9	27
α-helix	883-890	8
β-strand	896-906	11	27
α-helix	923-925	3
α-helix	926-928	3
α-helix	929-937	9
α-helix	938-942	5

Chain d: 3 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	21-26	6
α-helix	30-38	9
β-strand	41-46	6	17
β-strand	51-59	9	17
β-strand	65-76	12	17
β-strand	90-101	12	17
α-helix	103-105	3
β-strand	106-111	6	17

Chains e and l: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	17-27	11
β-strand	30-35	6	17
β-strand	38-50	13	17
β-strand	56-65	10	17
β-strand	70-79	10	17
α-helix	81-83	3
β-strand	84-90	7	17

Chain E: 1 helix, 31 β-strands

Element	Residues	Length	Sheet
β-strand	61-63	3	29
β-strand	69-74	6	30
β-strand	80	1	31
β-strand	81-85	5	30
β-strand	90-93	4	30
β-strand	94	1	31
β-strand	101-105	5	30
β-strand	112-117	6	32
β-strand	123-128	6	32
β-strand	133-137	5	32
β-strand	143-146	4	32
β-strand	154-159	6	33
α-helix	160-161	2
β-strand	167-171	5	33
β-strand	176-180	5	33
β-strand	185-189	5	33
β-strand	196-201	6	34
β-strand	207-212	6	34
β-strand	217-221	5	34
β-strand	227-231	5	34
β-strand	240-243	4	35
β-strand	249-253	5	35
β-strand	259-263	5	35
β-strand	273-277	5	35
β-strand	291-293	3	36
β-strand	299-302	4	36
β-strand	304	1	29
β-strand	309-313	5	36
β-strand	319-323	5	36
β-strand	330-335	6	29
β-strand	341-346	6	29
β-strand	350-355	6	29

33 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029 (AlphaFold model)
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7 (AlphaFold model)
pre-mRNA	4	RNA	46	unidentified adenovirus
pre-mRNA	G	RNA	174	unidentified adenovirus
Crooked neck-like protein 1	J	protein	848	Homo sapiens	Q9BZJ0 (AlphaFold model)
Cell division cycle 5-like protein	L	protein	802	Homo sapiens	Q99459
Pre-mRNA-splicing factor SYF2	M	protein	243	Homo sapiens	O95926
Protein BUD31 homolog	N	protein	144	Homo sapiens	P41223
Pre-mRNA-splicing factor RBM22	O	protein	420	Homo sapiens	Q9NW64
Spliceosome-associated protein CWC15 homolog	P	protein	229	Homo sapiens	Q9P013
SNW domain-containing protein 1	R	protein	536	Homo sapiens	Q13573

28 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>7W59_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 2 (C), FASTA

>7W59_2 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 3 (E), FASTA

>7W59_3 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 4 (4), FASTA

>7W59_4 pre-mRNA (chains 4)
GAUCGGGCUCGCCACUCUUGGAUCGGAACCCGUCGGCCUCCGAACG

Sequence of entity 5 (G), FASTA

>7W59_5 pre-mRNA (chains G)
GUAAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGC
GCAGUAGUCCAGGGUUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAG
CUCGCGGUUGAGGACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCG

Sequence of entity 6 (J), FASTA

>7W59_6 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES

Sequence of entity 7 (L), FASTA

>7W59_7 Cell division cycle 5-like protein (chains L)
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKK
TEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDP
RKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEAR
RLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFR
KLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP
QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQD
RILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGL
LGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKA
VQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG
KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQV
WEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKIL
LGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQER
EKELQHRYADLLLEKETLKSKF

Sequence of entity 8 (M), FASTA

>7W59_8 Pre-mRNA-splicing factor SYF2 (chains M)
MAAIAASEVLVDSAEEGSLAAAAELAAQKREQRLRKFRELHLMRNEARKLNHQEVVEEDK
RLKLPANWEAKKARLEWELKEEEKKKECAARGEDYEKVKLLEISAEDAERWERKKKRKNP
DLGFSDYAAAQLRQYHRLTKQIKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEID
RMVIDLEKQIEKRDKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERG
TAV

Sequence of entity 9 (N), FASTA

>7W59_9 Protein BUD31 homolog (chains N)
MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYI
FDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICR
VPKSKLEVGRIIECTHCGCRGCSG

Sequence of entity 10 (O), FASTA

>7W59_10 Pre-mRNA-splicing factor RBM22 (chains O)
MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPG
VRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQN
MEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECP
YRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLG
DTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNV
KWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAV
VNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

Sequence of entity 11 (P), FASTA

>7W59_11 Spliceosome-associated protein CWC15 homolog (chains P)
MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFR
RELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEE
SDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQA
NFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

Sequence of entity 12 (R), FASTA

>7W59_12 SNW domain-containing protein 1 (chains R)
MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGD
GGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLV
PKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYT
PSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVK
EQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKARE
AVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRR
KERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDS
GFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSG
SDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE

Ligands and cofactors

ID	Name	Formula	Copies
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
MG	Magnesium ion	Mg	9
ZN	Zinc ion	Zn	6
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1

Primary citation

Mechanism of exon ligation by human spliceosome. Zhan, X., Lu, Y., Zhang, X. et al. Mol Cell (2022) 82:2769-2778.e4. DOI 10.1016/j.molcel.2022.05.021 · PubMed

Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
3LRU 1.85 Å, hPRP8 Non-Native Subdomain

Browse structure collections

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