7W6P: PDB entry 7W6P

Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a G protein biased agonist. Determined by electron microscopy at 3.47 Å resolution. Released 28 Sept 2022.

Method: Electron microscopy
Resolution: 3.47 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,408
Mol. weight: 170.19 kDa
Ligands: W96
Released: 28 Sept 2022

Explore 7W6P in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7W6P contains 28 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-29	23
β-strand	34-40	7	1
α-helix	46-53	8
β-strand	185-192	8	1
β-strand	195-202	8	1
α-helix	209-213	5
α-helix	214-216	3
β-strand	221-227	7	1
α-helix	243-255	13
β-strand	264-270	7	1
α-helix	297-309	13
β-strand	319-323	5	1
α-helix	331-351	21

Chain B: 4 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	7-24	18
α-helix	38-41	4
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	104-105	2	4
β-strand	111-114	4	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	157-161	5	5
β-strand	165-166	2	5
β-strand	180	1	5
β-strand	190-192	3	6
β-strand	198-201	4	6
β-strand	208-212	5	6
β-strand	217-222	6	6
β-strand	232-234	3	7
α-helix	235	1
β-strand	241-243	3	7
β-strand	249-253	5	7
β-strand	260-265	6	7
β-strand	275-276	2	8
β-strand	284	1	9
β-strand	287-288	2	8
β-strand	294-295	2	8
β-strand	297	1	10
β-strand	298	1	9
β-strand	304	1	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	10-23	14
α-helix	30-43	14

Chain H: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	11
β-strand	17-24	8	11
α-helix	29-31	3
β-strand	33-38	6	12
β-strand	46-51	6	12
β-strand	58-60	3	12
β-strand	65	1	11
β-strand	68-73	6	11
β-strand	78-84	7	11
β-strand	92-99	8	12
β-strand	111-117	7	12
β-strand	128-129	2	13
β-strand	134	1	14
β-strand	143	1	15
β-strand	146-149	4	13
β-strand	154	1	16
β-strand	160	1	16
β-strand	162-167	6	17
β-strand	174-178	5	17
β-strand	182-183	2	17
α-helix	184	1
β-strand	193-195	3	13
β-strand	199-202	4	13
β-strand	204	1	15
β-strand	214-219	6	17
β-strand	227	1	17
α-helix	228-230	3
β-strand	231	1	17
β-strand	232	1	14

Chain R: 12 helices, 0 β-strands

Element	Residues	Length
α-helix	47-73	27
α-helix	82-84	3
α-helix	85-107	23
α-helix	118-150	33
α-helix	153-159	7
α-helix	162-178	17
α-helix	211-216	6
α-helix	217-221	5
α-helix	222-241	20
α-helix	380-411	32
α-helix	423-440	18
α-helix	446-456	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(o) subunit alpha	A	protein	354	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	349	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv	H	protein	307	Mus musculus
Alpha-2A adrenergic receptor	R	protein	465	Homo sapiens	P08913 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7W6P_1 Guanine nucleotide-binding protein G(o) subunit alpha (chains A)
MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDG
FSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPF
SAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRV
KTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDET
TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDA
AAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY

Sequence of entity 2 (B), FASTA

>7W6P_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTL
RGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNY
VACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDI
ETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINA
ICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDD
FNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>7W6P_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (H), FASTA

>7W6P_4 scFv (chains H)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFADVQLVESGGGLVQPGGSRKLSC
SASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQ
MTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELKGSLEVLFQGPAAAH
HHHHHHH

Sequence of entity 5 (R), FASTA

>7W6P_5 Alpha-2A adrenergic receptor (chains R)
MFRQEQPLAEGSFAPMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLML
LTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKAW
CEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVISAVIS
FPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKR
RTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGP
RDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTP
AAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPR
TLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV

Ligands and cofactors

ID	Name	Formula	Copies
W96	N-pyridin-4-ylisoquinolin-4-amine	C14 H11 N3	1

Primary citation

Structure-based discovery of nonopioid analgesics acting through the alpha 2A -adrenergic receptor. Fink, E.A., Xu, J., Hubner, H. et al. Science (2022) 377:eabn7065-eabn7065. DOI 10.1126/science.abn7065 · PubMed

Other PDB entries of the same protein (UniProt P09471 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BSB 2.32 Å, Global reconstruction of DRD2 bound to LSD in complex with a mini-GoA and scFv16…
9VJE 2.47 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with buspirone
9K20 2.65 Å, Cryo-EM structure of ATP-bound P2Y purinoceptor 2-miniGo-scFv16 complex
9VJ5 2.69 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with gepirone
8E9X 2.7 Å, CryoEM structure of miniGo-coupled hM4Di in complex with DCZ
9T88 2.74 Å, Cryo-EM structure of ESG-2-37-bound D3 dopamine receptor with mini-Go
9VNF 2.74 Å, Cryo-EM structure of 5-HT1AR-GoA in complex with (S)-pindolol
8YN7 2.77 Å, Cryo-EM structure of histamine H3 receptor in complex with immethridine and miniGo
8YN8 2.77 Å, Cryo-EM structure of histamine H3 receptor in complex with proxyfan and miniGo
9KVP 2.79 Å, Structure of SB290157 bound to human C3aR in complex with Go (Full map)
7W2Z 2.8 Å, Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex
8XXH 2.8 Å, Structure of CXCR2 bound to CXCL2 (CXCR2-CXCL2-Go Full map)

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