7XMS: Somatostatin receptor 4

CryoEM structure of somatostatin receptor 4 (SSTR4) in complex with Gi1 and its endogeneous ligand SST-14. Determined by electron microscopy at 2.9 Å resolution. Released 3 Aug 2022.

Method
Electron microscopy
Resolution
2.9 Å
Organisms
Homo sapiens, Mus musculus
Chains
6
Atoms
8,370
Mol. weight
160.87 kDa
Released
3 Aug 2022

Explore 7XMS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XMS contains 27 α-helices and 64 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

ElementResiduesLengthSheet
α-helix9-3224
β-strand34-3852
α-helix49-524
β-strand185-19062
β-strand195-20062
α-helix208-2103
β-strand220-22672
α-helix242-25413
β-strand263-26972
α-helix271-2788
α-helix296-30813
β-strand320-32342
α-helix330-35122
Chain B: 2 helices, 30 β-strands
ElementResiduesLengthSheet
α-helix38-403
β-strand47-5153
β-strand58-6254
β-strand69-7464
β-strand78-8364
β-strand88-9474
β-strand102-10545
β-strand111-11555
β-strand121-12555
β-strand135-13735
β-strand148-15146
β-strand156-16056
β-strand166-17056
β-strand176-18056
β-strand18717
β-strand191-19228
β-strand198-20258
β-strand20317
β-strand208-21258
β-strand218-22258
β-strand229-23249
β-strand240-24569
β-strand249-25469
β-strand259-26579
α-helix2721
β-strand273-278610
β-strand285-289510
β-strand294-297410
β-strand304-308510
β-strand315-32063
β-strand327-33153
β-strand336-33943
Chain C: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix31-4313
Chain R: 13 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix48-7124
α-helix72-765
α-helix81-10929
α-helix116-14934
α-helix151-1544
α-helix160-17819
α-helix180-1834
β-strand185-18731
β-strand198-20031
α-helix205-21612
α-helix217-2215
α-helix222-24423
α-helix252-28130
α-helix293-31220
α-helix316-3216
Chain S: 4 helices, 26 β-strands
ElementResiduesLengthSheet
β-strand5-7311
β-strand12112
β-strand21-23311
β-strand35-39513
β-strand45-51713
β-strand58-60313
β-strand68-73611
α-helix74-763
β-strand78-83611
α-helix88-903
β-strand93-95313
β-strand110114
β-strand115-116213
β-strand119112
β-strand146-147215
β-strand155-157316
β-strand166117
β-strand172117
β-strand174-177418
β-strand186114
β-strand187-190418
β-strand194-195218
β-strand203-206416
β-strand213-216416
α-helix221-2233
β-strand226-227215
β-strand228-231418
α-helix2371
β-strand238113
β-strand243-245315

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Somatostatin receptor type 4Rprotein375Homo sapiensP31391 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1Aprotein354Homo sapiensP63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1Bprotein351Homo sapiensP62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2Cprotein71Homo sapiensP59768 (AlphaFold model)
signle chain viable fragment of antibodySprotein292Mus musculus
Somatostatin-14Lprotein14Homo sapiensP61278
Sequence of entity 1 (R), FASTA
>7XMS_1 Somatostatin receptor type 4 (chains R)
DYKDDDDGAPSAPSTLPPGGEEGLGTAWPSAANASSAPAEAEEAVAGPGDARAAGMVAIQ
CIYALVCLVGLVGNALVIFVILRYAKMKTATNIYLLNLAVADELFMLSVPFVASSAALRH
WPFGSVLCRAVLSVDGLNMFTSVFCLTVLSVDRYVAVVHPLRAATYRRPSVAKLINLGVW
LASLLVTLPIAIFADTRPARGGQAVACNLQWPHPAWSAVFVVYTFLLGFLLPVLAIGLCY
LLIVGKMRAVALRAGWQQRRRSEKKITRLVLMFVVVFVLCWMPFYVVQLLNLFVTSLDAT
VNHVSLILSYANSCANPILYGFLSDNFRRFFQRVLCLEFLEVLFQGPWSHPQFEKGGGSG
GGSGGSAWSHPQFEK
Sequence of entity 2 (A), FASTA
>7XMS_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKCTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 3 (B), FASTA
>7XMS_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 4 (C), FASTA
>7XMS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (S), FASTA
>7XMS_5 signle chain viable fragment of antibody (chains S)
MKTIIALSYIFCLVFADYKDDDDGAPSEPDVQLVESGGGLVQPGGSRKLSCSASGFAFSS
FGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDT
AMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTP
GESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTA
FTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELEFLEVLFQGPHHHHHH
Sequence of entity 6 (L), FASTA
>7XMS_6 Somatostatin-14 (chains L)
AGCKNFFWKTFTSC

Primary citation

Structural insights into ligand recognition and selectivity of somatostatin receptors. Zhao, W., Han, S., Qiu, N. et al. Cell Res (2022) 32:761-772. DOI 10.1038/s41422-022-00679-x · PubMed

Other PDB entries of the same protein (UniProt P31391 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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