Cryo-EM structure of human DICER-pre-miRNA in a dicing state. Determined by electron microscopy at 3.04 Å resolution. Released 8 Mar 2023.
Explore 7XW2 in 3D Show helices and sheets RCSB PDB PDBe
7XW2 contains 37 α-helices and 31 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 747-750 | 4 | 1 |
| α-helix | 755-757 | 3 | |
| β-strand | 769-770 | 2 | 2 |
| β-strand | 776 | 1 | 3 |
| β-strand | 780 | 1 | 3 |
| α-helix | 781-782 | 2 | |
| β-strand | 801-804 | 4 | 4 |
| α-helix | 809-812 | 4 | |
| β-strand | 816-820 | 5 | 3 |
| β-strand | 823-827 | 5 | 3 |
| β-strand | 833-834 | 2 | 2 |
| α-helix | 840-851 | 12 | |
| α-helix | 852-857 | 6 | |
| β-strand | 866-867 | 2 | 5 |
| β-strand | 878-882 | 5 | 4 |
| β-strand | 891-892 | 2 | 4 |
| β-strand | 930-934 | 5 | 6 |
| α-helix | 942-944 | 3 | |
| β-strand | 945-947 | 3 | 6 |
| β-strand | 951 | 1 | 7 |
| α-helix | 969-975 | 7 | |
| α-helix | 985-986 | 2 | |
| β-strand | 987 | 1 | 7 |
| β-strand | 988-989 | 2 | 8 |
| β-strand | 990-992 | 3 | 6 |
| α-helix | 993-994 | 2 | |
| α-helix | 1018-1027 | 10 | |
| β-strand | 1032-1033 | 2 | 8 |
| β-strand | 1038-1044 | 7 | 6 |
| α-helix | 1045-1052 | 8 | |
| α-helix | 1054-1060 | 7 | |
| α-helix | 1061-1065 | 5 | |
| α-helix | 1069-1073 | 5 | |
| α-helix | 1294-1297 | 4 | |
| β-strand | 1302 | 1 | 9 |
| α-helix | 1303 | 1 | |
| α-helix | 1316-1334 | 19 | |
| α-helix | 1340-1350 | 11 | |
| α-helix | 1353-1363 | 11 | |
| α-helix | 1365-1368 | 4 | |
| β-strand | 1370 | 1 | 9 |
| α-helix | 1376-1379 | 4 | |
| β-strand | 1385-1386 | 2 | 5 |
| β-strand | 1551-1554 | 4 | 1 |
| α-helix | 1556-1574 | 19 | |
| α-helix | 1578-1586 | 9 | |
| α-helix | 1666-1673 | 8 | |
| α-helix | 1681-1685 | 5 | |
| β-strand | 1688 | 1 | 10 |
| α-helix | 1702-1721 | 20 | |
| α-helix | 1729-1740 | 12 | |
| α-helix | 1742-1751 | 10 | |
| α-helix | 1755-1757 | 3 | |
| β-strand | 1759 | 1 | 10 |
| α-helix | 1765-1776 | 12 | |
| α-helix | 1803-1805 | 3 | |
| α-helix | 1806-1811 | 6 | |
| α-helix | 1814-1822 | 9 | |
| α-helix | 1827-1848 | 22 | |
| α-helix | 1853-1860 | 8 | |
| β-strand | 1867 | 1 | 11 |
| α-helix | 1868-1870 | 3 | |
| β-strand | 1871-1872 | 2 | 12 |
| β-strand | 1878-1879 | 2 | 12 |
| β-strand | 1881-1883 | 3 | 11 |
| β-strand | 1889-1891 | 3 | 11 |
| β-strand | 1894 | 1 | 12 |
| α-helix | 1897-1911 | 15 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Endoribonuclease Dicer | A | protein | 1922 | Homo sapiens | Q9UPY3 (AlphaFold model) |
| RNA (73-mer) | C | RNA | 73 | Homo sapiens |
>7XW2_1 Endoribonuclease Dicer (chains A) MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNT IVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLA ILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDL VVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISK QILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCE EHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDE DEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFIT GHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLP TEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETD IDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETV KYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTP LPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSL QMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDI EKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKF PSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKR KAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGV GVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRT SSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGN QLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAV MPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGD SFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNW LPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYED DFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFED FDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIA DCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVS CAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNK AYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALV NNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEE DIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEM EPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVP NS
>7XW2_2 RNA (73-MER) (chains C) UGAGGUAGUAGGUUGUAUCGCUUUAGGGUCACACCCACCACUGGGAGAUAGCCAUACAAU CUACUGUCUUUCU
| ID | Name | Formula | Copies |
|---|---|---|---|
| CA | Calcium ion | Ca | 2 |
Structure of the human DICER-pre-miRNA complex in a dicing state. Lee, Y.Y., Lee, H., Kim, H. et al. Nature (2023) 615:331-338. DOI 10.1038/s41586-023-05723-3 · PubMed
Other PDB entries of the same protein (UniProt Q9UPY3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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