7XWO: Neurokinin A

Neurokinin A bound to active human neurokinin 2 receptor in complex with G324. Determined by electron microscopy at 2.7 Å resolution. Released 31 May 2023.

Method: Electron microscopy
Resolution: 2.7 Å
Organisms: Homo sapiens, Rattus norvegicus, Bos taurus
Chains: 6
Atoms: 8,725
Mol. weight: 178.36 kDa
Released: 31 May 2023

Explore 7XWO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XWO contains 36 α-helices and 61 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 21 helices, 0 β-strands

Element	Residues	Length
α-helix	30-47	18
α-helix	52-56	5
α-helix	57-61	5
α-helix	70-85	16
α-helix	89-95	7
α-helix	105-108	4
α-helix	112-122	11
α-helix	124-130	7
α-helix	132-135	4
α-helix	149-162	14
α-helix	164-167	4
α-helix	193-196	4
α-helix	198-201	4
α-helix	202-206	5
α-helix	207-225	19
α-helix	242-260	19
α-helix	264-267	4
α-helix	268-270	3
α-helix	285-294	10
α-helix	296-298	3
α-helix	299-309	11

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	12-28	17
α-helix	35-38	4
α-helix	43-44	2
β-strand	52-56	5	1
β-strand	63-68	6	2
β-strand	74-79	6	2
β-strand	83-88	6	2
β-strand	93-99	7	2
β-strand	105-110	6	3
β-strand	116-121	6	3
β-strand	126-130	5	3
β-strand	139-144	6	3
β-strand	156-157	2	4
β-strand	161-165	5	4
β-strand	171-175	5	4
β-strand	180-185	6	4
β-strand	192-197	6	5
β-strand	203-208	6	5
β-strand	213-217	5	5
β-strand	223-227	5	5
β-strand	234-239	6	6
β-strand	245-250	6	6
β-strand	255-259	5	6
β-strand	264-269	6	6
β-strand	278-283	6	7
β-strand	289-294	6	7
β-strand	299-303	5	7
β-strand	308-313	6	7
β-strand	320-325	6	1
β-strand	332-336	5	1
β-strand	341-344	4	1

Chain E: 1 helix, 24 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	8
β-strand	10-11	2	9
β-strand	17-24	8	8
α-helix	28-30	3
β-strand	32-38	7	10
β-strand	44-50	7	10
β-strand	57	1	10
β-strand	67-72	6	8
β-strand	77-82	6	8
β-strand	91-98	8	10
β-strand	114-116	3	10
β-strand	117-118	2	9
β-strand	141-142	2	11
β-strand	146-148	3	12
β-strand	155-160	6	11
β-strand	166	1	13
β-strand	172	1	13
β-strand	174-179	6	12
β-strand	186-189	4	12
β-strand	195	1	12
β-strand	203-207	5	11
β-strand	211-216	6	11
β-strand	225-231	7	12
β-strand	239	1	12
β-strand	243-246	4	12

Chain F: 8 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	10-24	15
α-helix	28-31	4
β-strand	34-39	6	14
α-helix	46-51	6
β-strand	184-190	7	14
β-strand	195-201	7	14
β-strand	220-223	4	14
β-strand	224-226	3	15
α-helix	233-244	12
β-strand	253-254	2	14
β-strand	257-259	3	15
α-helix	261-269	9
α-helix	275-277	3
α-helix	299-318	20
β-strand	326	1	14
β-strand	329-330	2	15
α-helix	338-357	20

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-20	11
α-helix	29-42	14
α-helix	52-54	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Soluble cytochrome b562,Substance-K receptor	B	protein	505	Homo sapiens	P0ABE7 (AlphaFold model), P21452 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	344	Rattus norvegicus	P54311 (AlphaFold model)
scFv	E	protein	304	Rattus norvegicus
G324	F	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
His-lys-thr-asp-ser-phe-val-gly-leu-met-NH2	D	protein	11	Homo sapiens	P20366

Sequence of entity 1 (B), FASTA

>7XWO_1 Soluble cytochrome b562,Substance-K receptor (chains B)
MKTIIALSYIFCLVFADYKDDDDAKLQTMHHHHHHHHHHENLYFQGGTADLEDNWETLND
NLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQI
DDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLMGTCDIVTEANISSGPESNTTGITAF
SMPSWQLALWATAYLALVLVAVTGNAIVIWIILAHRRMRTVTNYFIVNLALADLCMAAFN
AAFNFVYASHNIWYFGRAFCYFQNLFPITAMFVSIYSMTAIAADRYMAIVHPFQPRLSAP
STKAVIAGIWLVALALASPQCFYSTVTMDQGATKCVVAWPEDSGGKTLLLYHLVVIALIY
FLPLAVMFVAYSVIGLTLWRRAVPGHQAHGANLRHLQAMKKFVKTMVLVVLTFAICWLPY
HLYFILGSFQEDIYCHKFIQQVYLALFWLAMSSTMYNPIIYCCLNHRFRSGFRLAFRCCP
WVTPTKEDKLELTPTTSLSKLVEKY

Sequence of entity 2 (C), FASTA

>7XWO_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
GSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (E), FASTA

>7XWO_3 scFv (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFAVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSADIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELVDENLYFQGASHHHH
HHHH

Sequence of entity 4 (F), FASTA

>7XWO_4 G324 (chains F)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
DSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V

Sequence of entity 5 (G), FASTA

>7XWO_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (D), FASTA

>7XWO_6 HIS-LYS-THR-ASP-SER-PHE-VAL-GLY-LEU-MET-NH2 (chains D)
HKTDSFVGLMA

Primary citation

Structural insights into the activation of neurokinin 2 receptor by neurokinin A. Sun, W., Yuan, Q., Zhang, H. et al. Cell Discov (2022) 8:72-72. DOI 10.1038/s41421-022-00437-8 · PubMed

Other PDB entries of the same protein (UniProt P0ABE7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6DYF 1.1 Å, Cu(II)-bound structure of the engineered cyt cb562 variant, CH3Y
5YO6 1.2 Å, Crystal Structure of B562RIL with engineered disulfide bond T9C-A36C
4JEA 1.22 Å, Crystal structure of an engineered Zn-RIDC1 construct with four interfacial disulfide…
7LSJ 1.26 Å, Cu-bound crystal structure of the engineered cyt cb562 variant, DiCyt2 - H63A,…
7MK4 1.27 Å, Co-bound crystal structure of the engineered cyt cb562 variant, DiCyt2
6DYC 1.33 Å, Co(II)-bound structure of the engineered cyt cb562 variant, CH3
5YO4 1.37 Å, Crystal Structure of B562RIL with engineered disulfide bond K27C-A79C
256B 1.4 Å, Improvement of the 2.5 Å resolution model of cytochrome B562 by redetermining the…
6OT4 1.4 Å, Bimetallic dodecameric cage design 2 (BMC2) from cytochrome cb562
7LRV 1.4 Å, Ni-bound crystal structure of the engineered cyt cb562 variant, DiCyt2, crystallized in…
9PQ4 1.48 Å, Bi-bound structure of the H77C variant of TriCyt2
6DYG 1.49 Å, Fe(II)-bound structure of the engineered cyt cb562 variant, CH3Y

Browse structure collections

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