7XXI: Purinergic receptor P2Y12R

Cryo-EM structure of the purinergic receptor P2Y12R in complex with 2MeSADP and Gi. Determined by electron microscopy at 3.0 Å resolution. Released 7 Jun 2023.

Method: Electron microscopy
Resolution: 3.0 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,002
Mol. weight: 130.75 kDa
Ligands: 6AD
Released: 7 Jun 2023

Explore 7XXI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XXI contains 28 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	23-26	4
α-helix	27-52	26
α-helix	58-85	28
α-helix	89-93	5
α-helix	94-95	2
α-helix	96-100	5
α-helix	101-127	27
α-helix	135-161	27
α-helix	181-223	43
α-helix	231-247	17
α-helix	250-264	15
α-helix	272-288	17
α-helix	290-301	12
α-helix	303-312	10

Chain B: 7 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	1
α-helix	46-50	5
β-strand	188	1	1
β-strand	191	1	2
β-strand	196	1	2
β-strand	197-201	5	1
α-helix	213-216	4
β-strand	221-227	7	1
α-helix	243-255	13
β-strand	264-270	7	1
α-helix	272-279	8
α-helix	297-309	13
β-strand	320-323	4	1
α-helix	332-350	19

Chain C: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	135-139	5	5
β-strand	148-153	6	6
β-strand	156-160	5	6
β-strand	166-170	5	6
β-strand	176-180	5	6
β-strand	187-191	5	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	249-254	6	8
β-strand	259-263	5	8
β-strand	273-277	5	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-307	4	9
β-strand	317-320	4	3
β-strand	327-330	4	3
β-strand	336-339	4	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-21	15
α-helix	22-24	3
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
P2Y purinoceptor 12	A	protein	382	Homo sapiens	Q9H244 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-2	B	protein	355	Homo sapiens	P04899 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	346	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7XXI_1 P2Y purinoceptor 12 (chains A)
GAPQAVDNLTSAPGNTSLCTRDYKITQVLFPLLYTVLFFVGLITNGLAMRIFFQIRSKSN
FIIFLKNTVISDLLMILTFPFKILSDAKLGTGPLRTFVCQVTSVIFYFTMYISISFLGLI
TIDRYQKTTRPFKTSNPKNLLGAKILSVVIWAFMFLLSLPNMILTNRQPRDKNVKKCSFL
KSEFGLVWHEIVNYICQVIFWINFLIVIVCYTLITKELYRSYVRTRGVGKVPRKKVNVKV
FIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTLFYVKESTLWLTSLNACLDPFIY
FFLCKSFRNSLISMLKCPNSATSLSQDNRKKEQDGGDPNEETPMEFLEVLFQGPGSWSHP
QFEKGSGAGASAGSWSHPQFEK

Sequence of entity 2 (B), FASTA

>7XXI_2 Guanine nucleotide-binding protein G(i) subunit alpha-2 (chains B)
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVL
PDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRV
KTTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEE
MNRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEA
ASYIQSKFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (C), FASTA

>7XXI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGH
LAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVAC
GGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETG
QQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICF
FPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNC
NVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>7XXI_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
6AD	2-(methylsulfanyl)adenosine 5'-(trihydrogen diphosphate)	C11 H17 N5 O10 P2 S	1

Primary citation

Structural insights into signal transduction of the purinergic receptors P2Y1R and P2Y12R. Li, B., Han, S., Wang, M. et al. Protein Cell (2023) 14:382-386. DOI 10.1093/procel/pwac025 · PubMed

Other PDB entries of the same protein (UniProt Q9H244 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4PXZ 2.5 Å, Crystal structure of P2Y12 receptor in complex with 2MeSADP
4NTJ 2.62 Å, Structure of the human P2Y12 receptor in complex with an antithrombotic drug
7PP1 2.78 Å, Crystal structure of the P2Y12 receptor in complex with the inverse agonist selatogrel.
4PY0 3.1 Å, Crystal structure of P2Y12 receptor in complex with 2MeSATP

Browse structure collections

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