Cryo-EM structure of Nb29-alpha1AAR-miniGsq complex bound to oxymetazoline. Determined by electron microscopy at 2.92 Å resolution. Released 5 Jul 2023.
Explore 7YM8 in 3D Show helices and sheets RCSB PDB PDBe
7YM8 contains 21 α-helices and 20 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 25-52 | 28 | |
| α-helix | 60-74 | 15 | |
| α-helix | 75-79 | 5 | |
| α-helix | 80-89 | 10 | |
| α-helix | 96-128 | 33 | |
| α-helix | 142-158 | 17 | |
| α-helix | 177-179 | 3 | |
| α-helix | 185-189 | 5 | |
| α-helix | 190-194 | 5 | |
| α-helix | 195-210 | 16 | |
| α-helix | 264-285 | 22 | |
| α-helix | 288-295 | 8 | |
| α-helix | 305-325 | 21 | |
| α-helix | 327-329 | 3 | |
| α-helix | 331-340 | 10 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 15-20 | 6 | 1 |
| α-helix | 28-32 | 5 | |
| β-strand | 52-58 | 7 | 1 |
| β-strand | 61-67 | 7 | 1 |
| α-helix | 78-81 | 4 | |
| β-strand | 87-93 | 7 | 1 |
| α-helix | 100-112 | 13 | |
| β-strand | 120-126 | 7 | 1 |
| α-helix | 129-132 | 4 | |
| α-helix | 170-183 | 14 | |
| β-strand | 193-197 | 5 | 1 |
| α-helix | 205-224 | 20 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 6-7 | 2 | 2 |
| β-strand | 11-13 | 3 | 3 |
| β-strand | 17-22 | 6 | 2 |
| β-strand | 34-39 | 6 | 4 |
| β-strand | 41 | 1 | 5 |
| β-strand | 43 | 1 | 5 |
| β-strand | 45-51 | 7 | 4 |
| β-strand | 56-58 | 3 | 4 |
| β-strand | 66-71 | 6 | 2 |
| β-strand | 76-82 | 7 | 2 |
| β-strand | 90-95 | 6 | 4 |
| β-strand | 106 | 1 | 4 |
| β-strand | 110-112 | 3 | 4 |
| β-strand | 113-115 | 3 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| miniGsq | B | protein | 246 | Homo sapiens | |
| alpha1A adrenergic receptor | A | protein | 480 | Homo sapiens | D9IEF7, P35348 (AlphaFold model) |
| nanobody 29 | D | protein | 144 | synthetic construct |
>7YM8_1 miniGsq (chains B) MGHHHHHHHHLEVLFQGPIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGS GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCKDIILQMNL REYNLV
>7YM8_2 alpha1A adrenergic receptor (chains A) MKTIIALSYIFCLVFADYKDDDDAMVFLSGQASDSSQCTQPPAPVQISKAILLGVILGGL ILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEVLGYWAFGR VFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMALLCVWALSLV ISIGPLFGWRQPAPEDETICQINEEPGYVLFSALGSFYLPLAIILVMYCRVYVVAKRESR GLKSGLNIFEMLRIDEGGGSGGDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAA LINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTW DAYLKFSREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPDFKPSETVFKIVFWLGYLN SCINPIIYPCSSQEFKKAFQNVLRIQCLCRKQSSKHALGYTLHPPSQAVEGQHHHHHHHH
>7YM8_3 nanobody 29 (chains D) MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQAGGSLRLSCAASGNISAHWKMGWYR QAPGKEREFVAGIGYANTNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAYS YYRDHSYWGQGTQVTVSSHHHHHH
Structural basis of alpha 1A -adrenergic receptor activation and recognition by an extracellular nanobody. Toyoda, Y., Zhu, A., Kong, F. et al. Nat Commun (2023) 14:3655-3655. DOI 10.1038/s41467-023-39310-x · PubMed
Other PDB entries of the same protein (UniProt D9IEF7), best resolution first:
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