Cryo-EM structure of Nb29-alpha1AAR-miniGsq complex bound to noradrenaline. Determined by electron microscopy at 3.52 Å resolution. Released 5 Jul 2023.
Explore 7YMH in 3D Show helices and sheets RCSB PDB PDBe
7YMH contains 22 α-helices and 14 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 27-49 | 23 | |
| α-helix | 60-74 | 15 | |
| α-helix | 75-79 | 5 | |
| α-helix | 80-89 | 10 | |
| α-helix | 97-128 | 32 | |
| α-helix | 146-158 | 13 | |
| α-helix | 160-162 | 3 | |
| α-helix | 166-168 | 3 | |
| α-helix | 184-189 | 6 | |
| α-helix | 190-194 | 5 | |
| α-helix | 195-210 | 16 | |
| α-helix | 264-273 | 10 | |
| α-helix | 275-297 | 23 | |
| α-helix | 305-328 | 24 | |
| α-helix | 332-338 | 7 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 17-20 | 4 | 1 |
| α-helix | 28-32 | 5 | |
| β-strand | 53-58 | 6 | 1 |
| β-strand | 61-67 | 7 | 1 |
| α-helix | 77-80 | 4 | |
| β-strand | 85 | 1 | 1 |
| β-strand | 88-93 | 6 | 1 |
| α-helix | 101-111 | 11 | |
| β-strand | 121-126 | 6 | 1 |
| α-helix | 128-135 | 8 | |
| α-helix | 142-145 | 4 | |
| α-helix | 166-183 | 18 | |
| β-strand | 193-196 | 4 | 1 |
| α-helix | 206-224 | 19 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 33-39 | 7 | 2 |
| β-strand | 46-51 | 6 | 2 |
| β-strand | 56-58 | 3 | 2 |
| β-strand | 66-70 | 5 | 3 |
| β-strand | 77-81 | 5 | 3 |
| β-strand | 91-97 | 7 | 2 |
| β-strand | 104-105 | 2 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| alpha1A-adrenergic receptor | A | protein | 480 | Homo sapiens | D9IEF7, P35348 (AlphaFold model) |
| miniGsq | B | protein | 246 | Homo sapiens | |
| Nb29 | D | protein | 144 | synthetic construct |
>7YMH_1 alpha1A-adrenergic receptor (chains A) MKTIIALSYIFCLVFADYKDDDDAMVFLSGQASDSSQCTQPPAPVQISKAILLGVILGGL ILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEVLGYWAFGR VFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMALLCVWALSLV ISIGPLFGWRQPAPEDETICQINEEPGYVLFSALGSFYLPLAIILVMYCRVYVVAKRESR GLKSGLNIFEMLRIDEGGGSGGDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAA LINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTW DAYLKFSREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPDFKPSETVFKIVFWLGYLN SCINPIIYPCSSQEFKKAFQNVLRIQCLCRKQSSKHALGYTLHPPSQAVEGQHHHHHHHH
>7YMH_2 miniGsq (chains B) MGHHHHHHHHLEVLFQGPIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRILHGGS GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFNDCKDIILQMNL REYNLV
>7YMH_3 Nb29 (chains D) MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQAGGSLRLSCAASGNISAHWKMGWYR QAPGKEREFVAGIGYANTNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAYS YYRDHSYWGQGTQVTVSSHHHHHH
| ID | Name | Formula | Copies |
|---|---|---|---|
| E5E | Noradrenaline | C8 H12 N O3 | 1 |
Structural basis of alpha 1A -adrenergic receptor activation and recognition by an extracellular nanobody. Toyoda, Y., Zhu, A., Kong, F. et al. Nat Commun (2023) 14:3655-3655. DOI 10.1038/s41467-023-39310-x · PubMed
Other PDB entries of the same protein (UniProt D9IEF7), best resolution first:
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