7Z1K: SPOC domain of human SHARP

Name: PDB 7Z1K: Crystal structure of the SPOC domain of human SHARP (SPEN) in complex with RNA polymerase II CTD heptapeptide phosphorylated on Ser5
Published: 2022-12-07
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: transcription

Crystal structure of the SPOC domain of human SHARP (SPEN) in complex with RNA polymerase II CTD heptapeptide phosphorylated on Ser5. Determined by X-ray diffraction at 1.55 Å resolution. Released 7 Dec 2022.

Method: X-ray diffraction
Resolution: 1.55 Å
Organism: Homo sapiens
Chains: 2
Atoms: 1,472
Mol. weight: 20.45 kDa
Released: 7 Dec 2022

Explore 7Z1K in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7Z1K contains 9 α-helices and 9 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	3507-3515	9	1
β-strand	3518-3529	12	1
α-helix	3532-3537	6
β-strand	3547-3549	3	1
β-strand	3551-3554	4	1
α-helix	3557-3565	9
β-strand	3573-3580	8	1
α-helix	3585-3594	10
α-helix	3595-3600	6
α-helix	3601-3606	6
β-strand	3608-3615	8	1
α-helix	3616	1
β-strand	3622-3629	8	1
α-helix	3630	1
α-helix	3633-3642	10
α-helix	3644-3650	7
β-strand	3657-3663	7	1

Chain B: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	1-2	2	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Msx2-interacting protein	A	protein	172	Homo sapiens	Q96T58
Ser-tyr-ser-pro-thr-sep	B	protein	16	Homo sapiens	P24928 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7Z1K_1 Msx2-interacting protein (chains A)
GAMVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQR
MRLEATQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGII
NVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV

Sequence of entity 2 (B), FASTA

>7Z1K_2 SER-TYR-SER-PRO-THR-SEP (chains B)
PSYSPTSPSYSPTSPS

Primary citation

The SPOC domain is a phosphoserine binding module that bridges transcription machinery with co- and post-transcriptional regulators. Appel, L.M., Franke, V., Benedum, J. et al. Nat Commun (2023) 14:166-166. DOI 10.1038/s41467-023-35853-1 · PubMed

Other PDB entries of the same protein (UniProt Q96T58), best resolution first:

1OW1 1.8 Å, Crystal structure of the SPOC domain of the human transcriptional corepressor, SHARP.
4P6Q 2.0 Å, The crystal structure of the Split End protein SHARP adds a new layer of complexity to…
2RT5 Structural insights into the recruitment of SMRT by the co-repressor SHARP under…

Browse structure collections

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