8AHL: Crescentin filaments
Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and large box). Determined by electron microscopy at 4.1 Å resolution. Released 16 Aug 2023.
- Method
- Electron microscopy
- Resolution
- 4.1 Å
- Organisms
- Caulobacter vibrioides, Camelidae
- Chains
- 12
- Atoms
- 15,044
- Mol. weight
- 808.58 kDa
- Released
- 16 Aug 2023
Explore 8AHL in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8AHL contains 17 α-helices and 42 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 36-60 | 25 | |
| α-helix | 63-68 | 6 | |
| α-helix | 71-82 | 12 | |
| α-helix | 86-276 | 191 | |
Chain B: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-276 | 236 | |
Chains C and D: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 214-445 | 232 | |
Chain E: 2 helices, 12 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 791-793 | 3 | 1 |
| β-strand | 796 | 1 | 1 |
| β-strand | 807-810 | 4 | 2 |
| β-strand | 811-812 | 2 | 3 |
| α-helix | 817-818 | 2 | |
| β-strand | 820-824 | 5 | 2 |
| β-strand | 830-832 | 3 | 2 |
| β-strand | 841-845 | 5 | 1 |
| β-strand | 850-855 | 6 | 1 |
| α-helix | 860-862 | 3 | |
| β-strand | 864-866 | 3 | 3 |
| β-strand | 869-872 | 4 | 4 |
| β-strand | 875-878 | 4 | 4 |
| β-strand | 882-884 | 3 | 3 |
Chain F: 1 helix, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-7 | 4 | 5 |
| β-strand | 794-797 | 4 | 5 |
| β-strand | 807-812 | 6 | 6 |
| β-strand | 820-824 | 5 | 6 |
| β-strand | 831 | 1 | 6 |
| β-strand | 842-845 | 4 | 7 |
| β-strand | 850-853 | 4 | 7 |
| α-helix | 860-862 | 3 | |
| β-strand | 865-870 | 6 | 6 |
Chain G: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 40-192 | 153 | |
Chain H: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 32-192 | 161 | |
Chains I and J: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 328-445 | 118 | |
2 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Crescentin | A, B, C, D, G, H, I, J | protein | 460 | Caulobacter vibrioides | A0A0H3CD98 (AlphaFold model) |
| Crescentin-specific megabody MB13 | E, F, K, L | protein | 907 | Camelidae | P42592 (AlphaFold model) |
Sequence of entity 1 (A, B, C, D, G, H, I, J), FASTA
>8AHL_1 Crescentin (chains A, B, C, D, G, H, I, J)
MRLLSKNSRETKNGKPTVLGDEARAEAMQHQIESTQAIGQRYETIHGGLDSIGRVMEHLK
AIEPLIAEIRGPVSQEFEARRAEHAELIAVRANLDQAQRQIALIQAEEREVSARLAAAET
ALGESDARRQTQDAALEDNALEIDRLRNALLQSDLKVSSLDASLRDATARIEHLVQDVEG
LRVQAQDIDARRGDAEAALARANQDNALLGEEAATLKKRVDQAGLDLARLSRIETDLEAQ
LAAERARVQAVENALAAHQADSGRTIRGLESQVEANRAEISALQTRLETATGRADKLEEM
NGQISARLADSSAQQKAVERRAGDLNVALERALDRIRALEEEADGLRQRHAGVDTARATA
IERADQLAKSAVAQEKALKRAEERAQQLRARLDAMQEAQDQVRRDSATHEAKIAELQATI
ERLTSEAALAEGALEAARRDRSRLQMALLGASDGDVAASA
Sequence of entity 2 (E, F, K, L), FASTA
>8AHL_2 Crescentin-specific megabody MB13 (chains E, F, K, L)
EVQLQESGGGLVYKEETQSGLNNYARVVEKGQYDSLEIPAQVAASWESGRDDAAVFGFID
KEQLDKYVANGGKRSDWTVKFAENRSQDGTLLGYSLLQESVDQASYMYSDNHYLAEMATI
LGKPEEAKRYRQLAQQLADYINTCMFDPTTQFYYDVRIEDKPLANGCAGKPIVERGKGPE
GWSPLFNGAATQANADAVVKVMLDPKEFNTFVPLGTAALTNPAFGADIYWRGRVWVDQFW
FGLKGMERYGYRDDALKLADTFFRHAKGLTADGPIQENYNPLTGAQQGAPNFSWSAAHLY
MLYNDFFRKQASGGGSGGGGSGGGGSGNADNYKNVINRTGAPQYMKDYDYDDHQRFNPFF
DLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQDGKKVDFTLEAYSIP
GALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDGELLEKLEAKEGKPLSD
KTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEYQVHKSLPVQTEINGNRFT
SKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAFYLTASQQRWEEYLKKGLTNP
DATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVTGRWFSGNQTWPWDTWKQAFAMA
HFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAWNLSPERGGDGGNWNERNTKPS
LAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWLRNRDHNGNGVPEYGATRDKAHNTESG
EMLFTVKKDSLRLSCASSRSIDGINIMRWYRQAPGKQRGMVAVVTGWGSTNYVDSVKGRF
IISRDSAKDTVYLQMNNLKPEDTAVYSCNAIYRGSEYWGQGTQVTVSSGENLYFQGSHHH
HHHHHHH
Primary citation
Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin. Liu, Y., van den Ent, F., Lowe, J. Proc Natl Acad Sci U S A (2024) 121:e2309984121-e2309984121. DOI 10.1073/pnas.2309984121 · PubMed
Other PDB entries of the same protein (UniProt A0A0H3CD98 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8AFE 3.3 Å, Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and small box)
- 8AFH 3.9 Å, Cryo-EM structure of crescentin filaments (stutter mutant, C2, symmetry and small box)
- 8AJB 4.3 Å, Cryo-EM structure of crescentin filaments (stutter mutant, C2 symmetry and large box)
- 8AFL 4.4 Å, Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and small box)
- 8AFM 4.8 Å, Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and small box)
- 8AIA 5.1 Å, Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and large box)
- 8AIX 5.8 Å, Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and large box)
Browse structure collections
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