8DCS: Cyanopindolol-bound beta1-adrenergic receptor

Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with heterotrimeric Gs-protein. Determined by electron microscopy at 2.5 Å resolution. Released 27 Jul 2022.

Method: Electron microscopy
Resolution: 2.5 Å
Organisms: Bos taurus, Lama glama, Meleagris gallopavo
Chains: 5
Atoms: 7,900
Mol. weight: 163.35 kDa
Ligands: P32
Released: 27 Jul 2022

Explore 8DCS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8DCS contains 32 α-helices and 48 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	16-38	23
β-strand	40-46	7	11
β-strand	194-200	7	11
β-strand	203-209	7	11
α-helix	220-224	5
β-strand	229-235	7	11
α-helix	251-264	14
β-strand	273-278	6	11
α-helix	280-286	7
α-helix	299-302	4
α-helix	318-338	21
β-strand	345-349	5	11
α-helix	354-376	23

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-62	5	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	89-94	6	2
α-helix	95	1
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-153	8	4
β-strand	156-161	6	4
β-strand	166-170	5	4
β-strand	175-180	6	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	208-212	5	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	260-264	5	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	303-308	6	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	30-43	14
α-helix	56-58	3

Chain N: 3 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	8
β-strand	11-12	2	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
α-helix	62-64	3
β-strand	68-73	6	8
β-strand	78-83	6	8
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-124	3	10
β-strand	125-126	2	9

Chain R: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	45-67	23
α-helix	70-72	3
α-helix	76-89	14
α-helix	90-94	5
α-helix	95-104	10
α-helix	110-144	35
α-helix	149-152	4
α-helix	155-177	23
β-strand	183	1	12
α-helix	187-193	7
β-strand	203	1	12
α-helix	205-212	8
α-helix	213-217	5
α-helix	218-241	24
α-helix	248-315	33
α-helix	322-343	22
α-helix	347-356	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	339	Bos taurus	P62871 (AlphaFold model)
Nanobody 35	N	protein	140	Lama glama
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	384	Bos taurus	P04896 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Endolysin,Endolysin,Beta-1 adrenergic receptor chimera	R	protein	508	Meleagris gallopavo	P00720, P07700

Sequence of entity 1 (B), FASTA

>8DCS_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (N), FASTA

>8DCS_2 Nanobody 35 (chains N)
AAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSHHHHHHEPEA

Sequence of entity 3 (A), FASTA

>8DCS_3 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GPLAMGCLGNSKTEDQRNEEKGQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTI
VKQMRILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDY
ILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDY
VPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVAS
SSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIED
YFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTE
NIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 4 (G), FASTA

>8DCS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFSAIL

Sequence of entity 5 (R), FASTA

>8DCS_5 Endolysin,Endolysin,Beta-1 adrenergic receptor chimera (chains R)
MKTIIALSYIFCLVFADYKDDDDKLEVLFQGPNIFEMLRIDEGLRLKIYKDTEGYYTIGI
GHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYD
SLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRV
ITTFRTGTWDAYAAGAELLSQQWEAGMSLLMALVVLLIVAGNVLVIAAIGSTQRLQTLTN
LFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAI
DRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQDPG
CCDFVTNRAYAIASSIISFYIPLLIMIFVYLRVYREAKEQIRKIDRCEGRFREHKALKTL
GIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVFFNWLGYANSAFNPIIYCRSPDFRKA
FKRLLCFPRKADRRLEVLFQGPHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
P32	Cyanopindolol	C16 H21 N3 O2	1

Primary citation

Structures of beta 1 -adrenergic receptor in complex with Gs and ligands of different efficacies. Su, M., Paknejad, N., Zhu, L. et al. Nat Commun (2022) 13:4095-4095. DOI 10.1038/s41467-022-31823-1 · PubMed

Other PDB entries of the same protein (UniProt P62871 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5KDO 1.9 Å, Heterotrimeric complex of the 4 alanine insertion variant of the Gi alpha1 subunit and…
1GOT 2.0 Å, Heterotrimeric complex of a gt-alpha/gi-alpha chimera and the gt-beta-gamma subunits
3V5W 2.07 Å, Human G Protein-Coupled Receptor Kinase 2 in Complex with Soluble Gbetagamma Subunits…
1TBG 2.1 Å, Beta-gamma dimer of the heterotrimeric G-protein transducin
1GP2 2.3 Å, G protein heterotrimer GI_ALPHA_1 BETA_1 GAMMA_2 with GDP bound
5WG4 2.31 Å, Human GRK2 in complex with Gbetagamma subunits and CCG257284
6B20 2.34 Å, Crystal structure of a complex between G protein beta gamma dimer and an inhibitory…
1GG2 2.4 Å, G protein heterotrimer mutant GI_ALPHA_1(G203A) BETA_1 GAMMA_2 with GDP bound
2TRC 2.4 Å, Phosducin/transducin beta-gamma complex
4MK0 2.4 Å, Crystal structure of G protein-coupled receptor kinase 2 in complex with a a rationally…
6U7C 2.44 Å, Human GRK2 in complex with Gbetagamma subunits and CCG258747
3PVU 2.48 Å, Bovine GRK2 in complex with Gbetagamma subunits and a selective kinase inhibitor (CMPD101)

Browse structure collections

About this viewer

MolViewer shows 8DCS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.