8DP5: PEAK3/14-3-3 complex

Structure of the PEAK3/14-3-3 complex. Determined by electron microscopy at 3.1 Å resolution. Released 28 Jun 2023.

Method
Electron microscopy
Resolution
3.1 Å
Organism
Homo sapiens
Chains
6
Atoms
8,864
Mol. weight
266.91 kDa
Released
28 Jun 2023

Explore 8DP5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8DP5 contains 67 α-helices and 25 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 21 helices, 12 β-strands

ElementResiduesLengthSheet
α-helix131-15727
α-helix161-1655
β-strand170-17341
β-strand180-18121
β-strand185-194101
β-strand197-206101
α-helix2071
α-helix215-2217
α-helix225-2262
β-strand23012
β-strand233-23751
α-helix2381
β-strand251-25441
β-strand260-26122
α-helix262-2698
α-helix274-29825
β-strand300-30233
α-helix307-3093
β-strand310-31342
α-helix314-3152
α-helix323-3242
β-strand325-32842
α-helix331-3333
β-strand334-33633
α-helix345-3462
α-helix349-36012
α-helix364-3652
α-helix369-38416
α-helix388-39912
α-helix420-44122
α-helix443-4464
α-helix447-45610
α-helix461-47212
Chain B: 18 helices, 13 β-strands
ElementResiduesLengthSheet
α-helix131-15727
α-helix161-1655
β-strand170-17344
β-strand180-18124
β-strand185-194104
β-strand197-206104
α-helix2071
α-helix215-2239
α-helix225-2262
β-strand23015
β-strand233-23754
β-strand251-25444
β-strand260-26126
α-helix262-2698
α-helix277-29721
β-strand300-30237
β-strand311-31336
α-helix314-3152
α-helix323-3242
β-strand325-32736
β-strand32815
α-helix331-3333
β-strand334-33637
α-helix349-36214
α-helix364-3652
α-helix369-38416
α-helix388-39912
α-helix420-44021
α-helix443-4464
α-helix447-45711
α-helix461-47111
Chain C: 13 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix5-1713
α-helix21-3212
α-helix36-394
α-helix40-7031
α-helix75-10228
α-helix103-1075
α-helix108-1103
α-helix114-13219
α-helix138-16124
α-helix167-18216
α-helix187-20216
α-helix205-2073
α-helix210-23122
Chain D: 14 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix4-1613
α-helix20-3112
α-helix36-383
α-helix39-7032
α-helix76-10328
α-helix104-1085
α-helix109-1113
α-helix115-13521
α-helix139-16224
α-helix168-18013
α-helix181-1855
α-helix188-20316
α-helix206-2083
α-helix211-23222
Chain P: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix69-713

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Protein PEAK3A, Bprotein491Homo sapiensQ6ZS72 (AlphaFold model)
14-3-3 protein beta/alphaCprotein246Homo sapiensP31946 (AlphaFold model)
14-3-3 protein epsilonDprotein255Homo sapiensP62258 (AlphaFold model)
Protein PEAK3 fragmentE, Pprotein491Homo sapiensQ6ZS72 (AlphaFold model)
Sequence of entity 1 (A, B), FASTA
>8DP5_1 Protein PEAK3 (chains A, B)
MSSPEPPTEPPEPDNPTWSTQPTYSNLGQIRAHLLPSKACRLRTPGSLSTNPEPLPPPLP
KKILTRTQSLPTRRTLHPSSIQVQPPRRPFLGSHSVDKSQAAVGPACLPAELTFGPADAP
LGLSLRDLHSPEAVHTALAARQLQGLRTIYARLRARLMGGHPGPCHPGHSFRLLDSSPCA
ESGDALYYRVVRAHEDAWHILVAKVPKPGADVPHPWGLELQASLSPHFNLQGLCGLVPEG
TLPGAPWRGAVALAAEVPERTVAQWLAEACTQPPEEFVWAVALLLLQLSAALKFLEAWGA
ALVELRPENLLLVAPRGCATTGPPRLLLTDFGRVCLQPPGPPGSPGPHAPQLGSLLRALL
SLAAPSTTPLAAGLELLAAQLTRLRPSASRTRGALQALLWGPGPELRGRGAPLGPWLRAL
GPWLRVRRGLLVLRLAERAAGGEAPSLEDWLCCEYLAEATESSMGQALALLWDLEGGGGA
DYKDDDDKGPV
Sequence of entity 2 (C), FASTA
>8DP5_2 14-3-3 protein beta/alpha (chains C)
MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSS
WRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFY
LKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFY
YEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGD
AGEGEN
Sequence of entity 3 (D), FASTA
>8DP5_3 14-3-3 protein epsilon (chains D)
MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASW
RIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVF
YYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVF
YYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGE
EQNKEALQDVEDENQ
Sequence of entity 4 (E, P), FASTA
>8DP5_4 Protein PEAK3 fragment (chains E, P)
MSSPEPPTEPPEPDNPTWSTQPTYSNLGQIRAHLLPSKACRLRTPGSLSTNPEPLPPPLP
KKILTRTQSLPTRRTLHPSSIQVQPPRRPFLGSHSVDKSQAAVGPACLPAELTFGPADAP
LGLSLRDLHSPEAVHTALAARQLQGLRTIYARLRARLMGGHPGPCHPGHSFRLLDSSPCA
ESGDALYYRVVRAHEDAWHILVAKVPKPGADVPHPWGLELQASLSPHFNLQGLCGLVPEG
TLPGAPWRGAVALAAEVPERTVAQWLAEACTQPPEEFVWAVALLLLQLSAALKFLEAWGA
ALVELRPENLLLVAPRGCATTGPPRLLLTDFGRVCLQPPGPPGSPGPHAPQLGSLLRALL
SLAAPSTTPLAAGLELLAAQLTRLRPSASRTRGALQALLWGPGPELRGRGAPLGPWLRAL
GPWLRVRRGLLVLRLAERAAGGEAPSLEDWLCCEYLAEATESSMGQALALLWDLEGGGGA
DYKDDDDKGPV

Primary citation

Structural insights into regulation of the PEAK3 pseudokinase scaffold by 14-3-3. Torosyan, H., Paul, M.D., Forget, A. et al. Nat Commun (2023) 14:3543-3543. DOI 10.1038/s41467-023-38864-0 · PubMed

Other PDB entries of the same protein (UniProt Q6ZS72 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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