8F2A: Human Amylin3 Receptor

Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385 (Cluster 5 conformation). Determined by electron microscopy at 2.2 Å resolution. Released 2 Aug 2023.

Method: Electron microscopy
Resolution: 2.2 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 10,044
Mol. weight: 187.47 kDa
Ligands: NAG
Released: 2 Aug 2023

Explore 8F2A in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8F2A contains 55 α-helices and 58 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-39	28
β-strand	41-47	7	6
α-helix	53-58	6
β-strand	207-214	8	6
β-strand	217-224	8	6
α-helix	234-238	5
β-strand	243-249	7	6
α-helix	265-277	13
α-helix	280-282	3
β-strand	286-292	7	6
α-helix	294-303	10
α-helix	308-311	4
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-363	5	6
α-helix	371-390	20

Chain B: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	43	1	7
β-strand	47-51	5	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	88-94	7	9
α-helix	95	1
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	120-125	6	10
β-strand	135-140	6	10
β-strand	146-153	8	11
β-strand	156-161	6	11
β-strand	166-170	5	11
β-strand	175-180	6	11
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	208-212	5	12
β-strand	218-222	5	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	259-264	6	13
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	303-308	6	7
β-strand	315-320	6	8
β-strand	327-331	5	8
β-strand	336-339	4	8

Chain E: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	30-36	7
α-helix	38-47	10
α-helix	61-63	3
α-helix	65-75	11
α-helix	89-100	12
α-helix	113-115	3
α-helix	116-119	4
α-helix	121-140	20

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	11-21	11
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	14
β-strand	10-12	3	15
β-strand	18-25	8	14
α-helix	29-31	3
β-strand	34-39	6	15
β-strand	45-51	7	15
β-strand	58-60	3	15
α-helix	62-64	3
β-strand	68-73	6	14
β-strand	78-83	6	14
α-helix	88-90	3
β-strand	92-98	7	15
β-strand	118	1	15
β-strand	122-126	5	15

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	4	1	1
α-helix	5-7	3
α-helix	8-15	8
α-helix	28-30	3

Chain R: 23 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	43-61	19
β-strand	72	1	2
β-strand	75-76	2	3
β-strand	81-82	2	3
β-strand	85	1	2
β-strand	89-94	6	4
α-helix	95-96	2
β-strand	107-113	7	4
β-strand	117-118	2	4
α-helix	119	1
β-strand	120	1	5
α-helix	121	1
β-strand	127	1	5
β-strand	130	1	4
α-helix	132-135	4
α-helix	138-172	35
α-helix	179-200	22
α-helix	201-205	5
α-helix	209-214	6
α-helix	217-245	29
α-helix	246-250	5
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-329	31
α-helix	334-352	19
α-helix	355-358	4
β-strand	361	1	1
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-404	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 3	E	protein	149	Homo sapiens	O60896 (AlphaFold model)
Pramlintide analogue San385	P	protein	38	Homo sapiens	P10997 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>8F2A_1 Receptor activity-modifying protein 3 (chains E)
MKTIIALSYIFCLVFADYKDDDDKRAGGCNETGMLERLPLCGKAFADMMGKVDVWKWCNL
SEFIVYYESFTNCTEMEANVVGCYWPNPLAQGFITGIHRQFFSNCTVDRVHLEDPPDEVL
IPLIVIPVVLTVAMAGLVVWRSKRTDTLL

Sequence of entity 2 (P), FASTA

>8F2A_2 Pramlintide analogue San385 (chains P)
KCNTATCATQRLANFLVHKSNNFGPILPPTNVGSNTYX

Sequence of entity 3 (R), FASTA

>8F2A_3 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Sequence of entity 4 (A), FASTA

>8F2A_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>8F2A_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>8F2A_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>8F2A_7 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	4

Primary citation

Structural insight into selectivity of amylin and calcitonin receptor agonists. Cao, J., Belousoff, M.J., Gerrard, E. et al. Nat Chem Biol (2024) 20:162-169. DOI 10.1038/s41589-023-01393-4 · PubMed

Other PDB entries of the same protein (UniProt O60896 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
6UVA 2.3 Å, CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with…
6UUS 2.4 Å, CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with…
7TZF 2.4 Å, Human Amylin3 Receptor in complex with Gs and rat amylin peptide
9BTW 3.0 Å, Human Amylin3 Receptor in complex with Gs and cagrilintide

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