9BTW: Human Amylin3 Receptor

Human Amylin3 Receptor in complex with Gs and cagrilintide. Determined by electron microscopy at 3.0 Å resolution. Released 16 Apr 2025.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 9,713
Mol. weight: 182.33 kDa
Ligands: NAG
Released: 16 Apr 2025

Explore 9BTW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9BTW contains 50 α-helices and 56 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-38	27
β-strand	40-45	6	6
α-helix	53-58	6
β-strand	207-214	8	6
β-strand	217-224	8	6
α-helix	234-240	7
β-strand	243-248	6	6
α-helix	250-252	3
α-helix	265-277	13
β-strand	286-291	6	6
α-helix	294-302	9
α-helix	313-316	4
α-helix	325-327	3
α-helix	332-352	21
β-strand	359-363	5	6
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	7
β-strand	58-63	6	8
β-strand	69-74	6	8
β-strand	78-83	6	8
β-strand	89-94	6	8
β-strand	100-105	6	9
β-strand	111-116	6	9
β-strand	121-125	5	9
β-strand	134-139	6	9
β-strand	146-151	6	10
β-strand	156-161	6	10
β-strand	166-170	5	10
β-strand	175-180	6	10
β-strand	187-192	6	11
β-strand	198-203	6	11
β-strand	207-212	6	11
β-strand	217-223	7	11
β-strand	229-234	6	12
β-strand	240-245	6	12
β-strand	250-254	5	12
β-strand	259-264	6	12
β-strand	276-278	3	13
β-strand	284-288	5	13
β-strand	294-298	5	13
β-strand	304-306	3	13
β-strand	315-320	6	7
β-strand	327-331	5	7
β-strand	336-339	4	7

Chain E: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	31-51	21
α-helix	60-80	21
α-helix	85-86	2
α-helix	87-100	14
α-helix	113-115	3
α-helix	116-141	26

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	9-23	15
α-helix	30-43	14

Chain N: 3 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-8	6	14
β-strand	10-12	3	15
β-strand	17-25	9	14
α-helix	29-31	3
β-strand	34-39	6	15
β-strand	45-51	7	15
β-strand	58-60	3	15
α-helix	62-64	3
β-strand	68-73	6	14
β-strand	78-84	7	14
α-helix	88-90	3
β-strand	92-98	7	15
β-strand	118	1	15
β-strand	122-126	5	15

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-19	9
α-helix	28-31	4

Chain R: 23 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	42-61	20
β-strand	71-72	2	1
α-helix	73-74	2
β-strand	75-76	2	2
β-strand	81-82	2	2
β-strand	85-86	2	1
β-strand	90-94	5	3
α-helix	95-96	2
β-strand	107-111	5	3
β-strand	112	1	4
α-helix	113	1
β-strand	118	1	4
β-strand	120-121	2	5
β-strand	126-127	2	5
β-strand	130	1	3
α-helix	132-136	5
α-helix	138-172	35
α-helix	174-176	3
α-helix	179-204	26
α-helix	210-214	5
α-helix	217-245	29
α-helix	246-250	5
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	296-298	3
α-helix	299-330	32
α-helix	334-352	19
α-helix	354-358	5
α-helix	362-363	2
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-408	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 3	E	protein	149	Homo sapiens	O60896 (AlphaFold model)
Cagrilintide	P	protein	37	Homo sapiens	P10997 (AlphaFold model)
Calcitonin receptor	R	protein	462	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>9BTW_1 Receptor activity-modifying protein 3 (chains E)
MKTIIALSYIFCLVFADYKDDDDKRAGGCNETGMLERLPLCGKAFADMMGKVDVWKWCNL
SEFIVYYESFTNCTEMEANVVGCYWPNPLAQGFITGIHRQFFSNCTVDRVHLEDPPDEVL
IPLIVIPVVLTVAMAGLVVWRSKRTDTLL

Sequence of entity 2 (P), FASTA

>9BTW_2 Cagrilintide (chains P)
KCNTATCATQRLAEFLRHSSNNFGPILPPTNVGSNTP

Sequence of entity 3 (R), FASTA

>9BTW_3 Calcitonin receptor (chains R)
GPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQYKCYDRMQQLPAYQGEGPYCNRTWDGWLC
WDDTPAGVLSYQFCPDYFPDFDPSEKVTKYCDEKGVWFKHPENNRTWSNYTMCNAFTPEK
LKNAYVLYYLAIVGHSLSIFTLVISLGIFVFFRSLGCQRVTLHKNMFLTYILNSMIIIIH
LVEVVPNGELVRRDPVSCKILHFFHQYMMACNYFWMLCEGIYLHTLIVVAVFTEKQRLRW
YYLLGWGFPLVPTTIHAITRAVYFNDNCWLSVETHLLYIIHGPVMAALVVNFFFLLNIVR
VLVTKMRETHEAESHMYLKAVKATMILVPLLGIQFVVFPWRPSNKMLGKIYDYVMHSLIH
FQGFFVATIYCFCNNEVQTTVKRQWAQFKIQWNQRWGRRPSNRSARAAAAAAEAGDIPIY
ICHQELRNEPANNQGEESAEIIPLNIIEQESSAPAGLEVLFQ

Sequence of entity 4 (A), FASTA

>9BTW_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>9BTW_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>9BTW_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>9BTW_7 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2

Primary citation

Structural and dynamic features of cagrilintide binding to calcitonin and amylin receptors. Cao, J., Belousoff, M.J., Johnson, R.M. et al. Nat Commun (2025) 16:3389-3389. DOI 10.1038/s41467-025-58680-y · PubMed

Other PDB entries of the same protein (UniProt O60896 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2A 2.2 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385…
6UVA 2.3 Å, CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with…
6UUS 2.4 Å, CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with…
7TZF 2.4 Å, Human Amylin3 Receptor in complex with Gs and rat amylin peptide

Browse structure collections

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