8GHM: Hir1 WD40 domains and Asf1/H3/H4
Hir1 WD40 domains and Asf1/H3/H4. Determined by electron microscopy at 12.0 Å resolution. Released 10 Jul 2024.
- Method
- Electron microscopy
- Resolution
- 12.0 Å
- Organism
- Saccharomyces cerevisiae
- Chains
- 9
- Atoms
- 9,589
- Mol. weight
- 765.97 kDa
- Released
- 10 Jul 2024
Explore 8GHM in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8GHM contains 43 α-helices and 92 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 11 helices, 37 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-4 | 3 | 1 |
| β-strand | 10 | 1 | 2 |
| β-strand | 12-13 | 2 | 3 |
| β-strand | 16-17 | 2 | 3 |
| β-strand | 20-25 | 6 | 4 |
| β-strand | 31-36 | 6 | 4 |
| β-strand | 41-45 | 5 | 4 |
| α-helix | 46-58 | 13 | |
| α-helix | 63-65 | 3 | |
| α-helix | 66-68 | 3 | |
| β-strand | 73-75 | 3 | 4 |
| β-strand | 82-87 | 6 | 5 |
| β-strand | 94-98 | 5 | 5 |
| α-helix | 102 | 1 | |
| β-strand | 103-109 | 7 | 5 |
| α-helix | 115-117 | 3 | |
| β-strand | 126-133 | 8 | 5 |
| β-strand | 140-145 | 6 | 6 |
| β-strand | 151-156 | 6 | 6 |
| β-strand | 161-165 | 5 | 6 |
| β-strand | 171-175 | 5 | 6 |
| β-strand | 182-187 | 6 | 7 |
| β-strand | 193-198 | 6 | 7 |
| β-strand | 203-209 | 7 | 7 |
| β-strand | 215-222 | 8 | 7 |
| α-helix | 224-226 | 3 | |
| β-strand | 237 | 1 | 8 |
| β-strand | 239-240 | 2 | 9 |
| β-strand | 246-253 | 8 | 9 |
| β-strand | 258-264 | 7 | 9 |
| β-strand | 273-275 | 3 | 9 |
| β-strand | 281-291 | 11 | 8 |
| β-strand | 325-332 | 8 | 8 |
| β-strand | 337-341 | 5 | 8 |
| β-strand | 349-352 | 4 | 8 |
| β-strand | 359-364 | 6 | 1 |
| β-strand | 370-375 | 6 | 1 |
| β-strand | 379 | 1 | 2 |
| β-strand | 380-384 | 5 | 1 |
| α-helix | 385-386 | 2 | |
| α-helix | 392 | 1 | |
| β-strand | 393 | 1 | 8 |
| α-helix | 394-395 | 2 | |
| α-helix | 396-401 | 6 | |
| α-helix | 404-406 | 3 | |
| β-strand | 488-490 | 3 | 10 |
| β-strand | 493-496 | 4 | 10 |
| β-strand | 499 | 1 | 11 |
Chain F: 3 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 453-455 | 3 | 1 |
| α-helix | 457-460 | 4 | |
| α-helix | 464-466 | 3 | |
| β-strand | 473-476 | 4 | 8 |
| α-helix | 477-485 | 9 | |
| β-strand | 595-597 | 3 | 9 |
Chain G: 11 helices, 34 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-4 | 3 | 14 |
| β-strand | 10 | 1 | 15 |
| β-strand | 12-13 | 2 | 16 |
| β-strand | 16-17 | 2 | 16 |
| β-strand | 20-25 | 6 | 17 |
| β-strand | 31-36 | 6 | 17 |
| β-strand | 41-45 | 5 | 17 |
| α-helix | 46-58 | 13 | |
| α-helix | 63-65 | 3 | |
| α-helix | 66-68 | 3 | |
| β-strand | 73-75 | 3 | 17 |
| β-strand | 82-87 | 6 | 18 |
| β-strand | 94-98 | 5 | 18 |
| α-helix | 102 | 1 | |
| β-strand | 103-109 | 7 | 18 |
| α-helix | 115-117 | 3 | |
| β-strand | 126-133 | 8 | 18 |
| β-strand | 140-145 | 6 | 19 |
| β-strand | 151-156 | 6 | 19 |
| β-strand | 161-165 | 5 | 19 |
| β-strand | 171-175 | 5 | 19 |
| β-strand | 182-187 | 6 | 20 |
| β-strand | 193-198 | 6 | 20 |
| β-strand | 203-209 | 7 | 20 |
| β-strand | 215-222 | 8 | 20 |
| α-helix | 224-226 | 3 | |
| β-strand | 237 | 1 | 21 |
| β-strand | 239-240 | 2 | 22 |
| β-strand | 246-253 | 8 | 22 |
| β-strand | 258-264 | 7 | 22 |
| β-strand | 272-275 | 4 | 22 |
| β-strand | 281-291 | 11 | 21 |
| β-strand | 325-332 | 8 | 21 |
| β-strand | 337-341 | 5 | 21 |
| β-strand | 349-352 | 4 | 21 |
| β-strand | 359-364 | 6 | 14 |
| β-strand | 370-375 | 6 | 14 |
| β-strand | 379 | 1 | 15 |
| β-strand | 380-384 | 5 | 14 |
| α-helix | 385-386 | 2 | |
| α-helix | 392 | 1 | |
| β-strand | 393 | 1 | 21 |
| α-helix | 394-395 | 2 | |
| α-helix | 396-401 | 6 | |
| α-helix | 404-406 | 3 | |
Chain L: 4 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 453-455 | 3 | 14 |
| α-helix | 457-460 | 4 | |
| α-helix | 464-466 | 3 | |
| β-strand | 473-476 | 4 | 21 |
| α-helix | 477-485 | 9 | |
| β-strand | 576 | 1 | 12 |
| α-helix | 579-584 | 6 | |
| β-strand | 595-597 | 3 | 22 |
Chain M: 3 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 66-79 | 14 | |
| β-strand | 84-85 | 2 | 12 |
| α-helix | 87-114 | 28 | |
| β-strand | 119-120 | 2 | 13 |
| α-helix | 122-132 | 11 | |
Chain N: 5 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-29 | 3 | |
| α-helix | 32-41 | 10 | |
| β-strand | 46-47 | 2 | 13 |
| α-helix | 51-76 | 26 | |
| β-strand | 81-82 | 2 | 12 |
| α-helix | 84-93 | 10 | |
| α-helix | 97-98 | 2 | |
Chain O: 6 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 4-11 | 8 | 11 |
| β-strand | 16-17 | 2 | 10 |
| α-helix | 21 | 1 | |
| β-strand | 22-30 | 9 | 11 |
| α-helix | 37 | 1 | |
| β-strand | 38-45 | 8 | 10 |
| β-strand | 54-62 | 9 | 10 |
| β-strand | 68-76 | 9 | 11 |
| α-helix | 77-80 | 4 | |
| α-helix | 86-89 | 4 | |
| β-strand | 92-101 | 10 | 10 |
| β-strand | 104-117 | 14 | 10 |
| α-helix | 120-124 | 5 | |
| α-helix | 132-134 | 3 | |
| β-strand | 135-139 | 5 | 10 |
| β-strand | 145-148 | 4 | 10 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Protein HIR1 | A, G | protein | 840 | Saccharomyces cerevisiae | P32479 (AlphaFold model) |
| Histone H3 | M | protein | 136 | Saccharomyces cerevisiae | P61830 (AlphaFold model) |
| Histone H4 | N | protein | 103 | Saccharomyces cerevisiae | P02309 (AlphaFold model) |
| Histone chaperone | O | protein | 279 | Saccharomyces cerevisiae | P32447 (AlphaFold model) |
| Histone transcription regulator 3 | D, J | protein | 1648 | Saccharomyces cerevisiae | P47171 |
| Histone promoter control protein 2 | F, L | protein | 625 | Saccharomyces cerevisiae | Q01448 |
Sequence of entity 1 (A, G), FASTA
>8GHM_1 Protein HIR1 (chains A, G)
MKVVKFPWLAHREESRKYEIYTVDVSHDGKRLATGGLDGKIRIWSIDSILRCMELESLTP
EIPLPQDLQMPLCSMSRHTGSITCVKFSPDGKYLASGSDDRILLIWALDEEQSSQPAFGS
EHEREHWTVRKRLVAHDNDIQDICWAPDSSILVTVGLDRSVIVWNGSTFEKLKRFDVHQS
LVKGVVFDPANKYFATTSDDRTMKIFRYHKTGDISFTIEHIITEPFKESPLTTYFRRPSW
SPDGQHIAVPNATNGPVSSVAIVNRGTWDTNVSLIGHDAPTEVARFNPRLFERNAGVKQK
KDDDPENALVGQNDDKVHHFDKNIDSVVATAGQDKSLAVWSTSRPRPILVAFDIANKSIT
DMSWNPDGSLLFVASLDSSITLFKFENNELGKPIPLEKNMEQLYRYGVDKDSLDFPESIN
QLLLEDQTKSFKHTKISTSKLGENHPTLATNSASNQKDNNDASVSRSEHINILIPKRKKD
AILNKAVTLKSGKKRVAPTLISTSSSSPFSNGIKKPTLDSKRIENNVKSSTKTINSKNTL
LNVPEGVEKKISISSFPLPRLGIHSLIMGTKERSAWKISNSELENDDADNAGGKGSDGTS
NSIDDIAVLSEEENDFHRMTLNAKLTQEKIWSEEPTTRCLLQSDVIPDTDVVVLEGGSLD
DIAVLEIRNGVERSIQFDSEALLDNPTRILGYQGGKRTIETFIPEVIICAIGSKDCKCWC
LASANGSIYILSYNGQQRIPKICLGHKVIKMVTSSKYLLVLTERGLFFAWDLLDLKLVLR
NVPILPILNGQPIHGNKVRINKVIKCFRLDGSSCDLLLEVGDPKNVYKWTKDLGCWSLYK
Sequence of entity 2 (M), FASTA
>8GHM_2 Histone H3 (chains M)
MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTI
QKKDIKLARRLRGERS
Sequence of entity 3 (N), FASTA
>8GHM_3 Histone H4 (chains N)
MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISGLIYEEVRAVLK
SFLESVIRDSVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG
Sequence of entity 4 (O), FASTA
>8GHM_4 Histone chaperone (chains O)
MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI
LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE
ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE
DDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE
EEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN
Sequence of entity 5 (D, J), FASTA
>8GHM_5 Histone transcription regulator 3 (chains D, J)
MSMFNALNSNIEGEQYEAEEHSRELQIEQSFNILQDALIDLKNKDFEKSDSKFQELFQID
VVKPDRWGMYRNSSPTLDNLRYLCYRNRGMYYHLYLENNYERLNSQELVNCILKAVENLV
ESIQHSDADFAVTDLLARIFKSFNSVKLERLISEYEFTKQENLSLLLGRHRKFLLNDLTL
MMNNYVELTNKLLVPNLSDNTIFERYHLEKYKDIKPEPLAFGPILSRISEMKKQDEEIMK
KLDVFNVTLNEESWDEVAKALKNLLPSVKTSSLIGRNMDPYNEIEEPIEAVKFELSEAIN
NTPSLDRESERQEEEQDNESVRADDKSGNLAPSDIQTNEEARPNKRTDEHIDSTKPLQRS
SKRFKEREQENSKELVMDVHKRFFGEFNTLLSYIHILPFCDFDTFASKFIIGSSDKQPEK
FIPYTDLYECLKSWSSRYTDIFNQNDYLSSGSNENEELFQLNALLKSNAFDDKESFPRYL
NDLDSDHIRSFISEVNAGNLHFHQVRLKLLFKLLGTYDEGNGRRLIIDYLWESQLLKIVL
WFVFGIESNIFALINKNKRQCKYLALSIYELLVNHLGNIVEEITNKRIQGHKSADLKSQR
NKVEKRIRSWHTLLEQIADEKDKELYVHFQWTHYCFLQYTCDIVDSRLSETLTSLENTIK
DSDSSLDIAYPNYRHIPALNLNTVQSQKRKIRIIQNITVEDISEDTNSDTHSENHLETLE
KVLLHILHPSTNHSNIDEEMVSFIFNSPFLLKIRLWGVLFSSYVKKSSIQDVQRIYFHVL
DFMKGALTSPVYKESNPHGRHQMLLTVLTAIGYLSSQLTAILNSNRWESSDFVLEDYMFE
KLLQTFFFFYTVLFYESSAVNDVSNKSFFKRASKSSGKMKDIMIDLATLILYYYDLQAKL
RTPAEQGIETTELIWSLHTLFGHFHFCDASNGKFLDLAEKLLCQFINNDSFLQLKQILWC
RYHYAIASDNFSPDLHDTKAVEMEKIHSLPLGTYLIKLQYQNKNPYLSSSKTTLKQIMDN
IIEKIGDPSTLDNHIISRNSFLLNEYLSRPITADLLKHTFSGATSLYLTSPNDELQQGMT
AGLFYVSSLQSLGLYKMRKKSMQARPSELDSIIRMLKNDIIYNTNRFESWILLGKCYSYI
VEDDLIWTSDKITVPEKKDVIALTQRKAILCYLMAISIYYSKLDRTIDDKKIILEALDDL
GSMLISGYYNPMNKLCFSWKSSAENTMRLSETGEVVMEKTKKITTISDFNIEQSIFLCFN
RACSLSGDIKSQDDVFVLNWSSFYNLAKFFFKTDGGNNCKLVAKYITQGCQIAYESSPAK
DPIIEPHYLLVNACYKWVKRGVIGVNEALTLLSKDNQFFQEQEEFWVNDEGLAWDYQEKF
FFDKIIRLLRHLLSVDKKKWQHRPRYRIARILFDDLGDVNGALEEMDSLISAKSINKNLV
NIWKPDFERPGKHFIYTYQYLVLYLDLLFAIKDFNTTGLVIKKLRRFGSGTVNVNELLER
AINVYTQSAKIKLQLQDKSYVEQILPTLNYQEFLKISEQLNQVFDQGKYPEEISSGLKLA
FQLKKGHSGIAFDSVCLGIYFEYLYFPLARQDQSLTDVNDENNPALPSSGSVTSKSTPDP
TSKPSAIKKRVTKKEVFDRVRLLVDKIT
Sequence of entity 6 (F, L), FASTA
>8GHM_6 Histone promoter control protein 2 (chains F, L)
MDQKAIVLDNSKSGSKQTKSSGKMQTQTDTNAEVLNTDNSIKKETGSDSEDLFNKFSNKK
TNRKIPNIAEELAKNRNYVKGASPSPIIISGSSSTSPSGPSSSSTNPMGIPTNRFNKNTV
ELYQHSPSPVMTTNKTDTEEKRQNNRNMDNKNTPERGSSSFAAKQLKISSLLTISSNEDS
KTLHINDTNGNKNSNAASNNIPSAYAELHTEGNSIESLIKPPSSPRNKSLTPKVILPTQN
MDGTIAKDPHLGDNTPGILIAKTSSPVNLDVESTAQSLGKFNKSTNSLKAALTKAPAEKV
SLKRSISSVTNSDSNISSSKKPTSEKAKKSSSASAILPKPTTTKTSKKAASNSSDSTRKK
NASNKTTSAIKKESNAGSKLNTVKKENSSLSSIKATEKEKDKGGNSTEAKNSTSNVRKEP
TAKSPKRLVAAPTVSPPKILQTAETKAKEPSILIDVPLYQADTNDYLDENGQVIFNLSTL
IKEKYHPKSKELAQLKDSKRNLLMQLSDHSNGSLEKEKDEEGDVIELDDDEDMEEDEGEI
DTETNTVTTTISPKKKSHPMKGKNLIGKYDVEDPFIDDSELLWEEQRAATKDGFFVYFGP
LIEKGHYASLERANGTMKRGGVKNK
Primary citation
Structure of the Hir histone chaperone complex. Kim, H.J., Szurgot, M.R., van Eeuwen, T. et al. Mol Cell (2024) 84:2601-2617.e12. DOI 10.1016/j.molcel.2024.05.031 · PubMed
Other PDB entries of the same protein (UniProt P32479 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8GHL 2.96 Å, the Hir complex core
- 8GHN 2.96 Å, Composite model of the yeast Hir Complex with Asf1/H3/H4
Browse structure collections
About this viewer
MolViewer shows 8GHM directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.