8GOH: LacI C-terminal tetramerization helix

Homotetramic Antiparallel Coiled-Coil of 23-residues LacI C-terminal tetramerization helix. Determined by X-ray diffraction at 1.1 Å resolution. Released 30 Aug 2023.

Method: X-ray diffraction
Resolution: 1.1 Å
Organism: Escherichia coli
Chains: 8
Atoms: 1,489
Mol. weight: 20.7 kDa
Released: 30 Aug 2023

Explore 8GOH in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8GOH contains 8 α-helices and 6 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B and G: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	4-18	15
β-strand	22	1	1

Chains C, D and E: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	4-19	16
β-strand	22	1	1

Chains F and H: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	4-18	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
LacI C-terminal tetramerization helix	A, B, C, D, E, F, G, H	protein	23	Escherichia coli	P03023 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H), FASTA

>8GOH_1 LacI C-terminal tetramerization helix (chains A, B, C, D, E, F, G, H)
TASPRALADSLMQLAYQVSRLSS

Primary citation

Design of interchain Glu-Arg salt-bridges stabilizing heterotetrameric coiled-coil derived from Lac repressor. Otsuka, Y., Osaki, A., Nakamura, Y. et al. To be published.

Other PDB entries of the same protein (UniProt P03023 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1JYE 1.7 Å, Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution
2P9H 2.0 Å, High resolution structure of the Lactose Repressor bound to IPTG
3EDC 2.1 Å, Crystal Structure of a 1.6-hexanediol Bound Tetrameric Form of Escherichia coli…
1EFA 2.6 Å, Crystal structure of the lac repressor dimer bound to operator and the anti-inducer onpf
1TLF 2.6 Å, Unprecedented quaternary structure of E. Coli lac repressor core tetramer: implications…
1LBI 2.7 Å, Lac repressor
4RZS 2.71 Å, Lac repressor engineered to bind sucralose, unliganded tetramer
1JYF 3.0 Å, Structure of the Dimeric Lac Repressor with an 11-residue C-terminal Deletion.
4RZT 3.1 Å, Lac repressor engineered to bind sucralose, sucralose-bound tetramer
1LBH 3.2 Å, Intact lactose operon repressor with gratuitous inducer iptg
2PAF 3.5 Å, Crystal Structure of the Lactose Repressor bound to anti-inducer ONPF in induced state
2PE5 3.5 Å, Crystal Structure of the Lac Repressor bound to ONPG in repressed state

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