8GSW: Human cardiac alpha actin A108G mutant

Crystal structure of human cardiac alpha actin A108G mutant (AMPPNP state) in complex with fragmin F1 domain. Determined by X-ray diffraction at 1.4 Å resolution. Released 8 Mar 2023.

Method: X-ray diffraction
Resolution: 1.4 Å
Organisms: Homo sapiens, Physarum polycephalum
Chains: 2
Atoms: 4,995
Mol. weight: 62.81 kDa
Ligands: ANP, CA, PO4, MG
Released: 8 Mar 2023

Explore 8GSW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8GSW contains 34 α-helices and 30 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	24	1	2
β-strand	29-32	4	1
β-strand	35-38	4	3
β-strand	53-54	2	3
α-helix	55-60	6
α-helix	62-64	3
β-strand	65-68	4	3
β-strand	71-72	2	4
β-strand	75-76	2	4
α-helix	79-87	9
α-helix	88-94	7
α-helix	98-100	3
β-strand	103-107	5	1
β-strand	112	1	5
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	6
β-strand	160-166	7	6
β-strand	169-170	2	6
α-helix	172-174	3
β-strand	176-178	3	6
α-helix	182-196	15
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	7
β-strand	247-250	4	7
α-helix	253-259	7
α-helix	264-267	4
α-helix	272-273	2
α-helix	274-283	10
α-helix	287-294	8
β-strand	297-300	4	6
α-helix	302-305	4
α-helix	309-320	12
β-strand	329-330	2	6
α-helix	335-337	3
α-helix	338-346	9
α-helix	350-353	4
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	369-373	5

Chain B: 10 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	12	1	5
α-helix	20-30	11
α-helix	34-36	3
β-strand	44-51	8	8
β-strand	54-57	4	8
α-helix	58-59	2
α-helix	60-62	3
β-strand	65-67	3	9
β-strand	71-78	8	8
β-strand	88-95	8	8
α-helix	101-117	17
α-helix	122	1
β-strand	123-128	6	8
α-helix	134-137	4
β-strand	146-148	3	9
α-helix	152-154	3
α-helix	156	1
β-strand	157	1	2
α-helix	158-159	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha cardiac muscle 1	A	protein	391	Homo sapiens	P68032 (AlphaFold model)
Actin-binding protein fragmin P	B	protein	160	Physarum polycephalum	Q94707 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8GSW_1 Actin, alpha cardiac muscle 1 (chains A)
MGWSHPQFEKGGIEGRDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVM
VGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTL
LTEGPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVP
IYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFE
NEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMK
CDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGS
ILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Sequence of entity 2 (B), FASTA

>8GSW_2 Actin-binding protein fragmin P (chains B)
MQKQKEYNIADSNIANLGTELEKKVKLEASQHEDAWKGAGKQVGVEIWRIQQFKVVPVPK
KHHGSFYTGDSYIVLSTYHPKTNPDKLAYDVHFWLGAFTTQDEAGTAAYKTVELDDYLGG
LPVQYREVQGYESERFLSLFPKGGLRILDGGVETGFHHVE

Ligands and cofactors

ID	Name	Formula	Copies
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	1
CA	Calcium ion	Ca	2
PO4	Phosphate ion	O4 P	1
MG	Magnesium ion	Mg	1

Water and common crystallization additives (EDO) are not listed.

Primary citation

Mutagenic analysis of actin reveals the mechanism of His161 flipping that triggers ATP hydrolysis. Iwasa, M., Takeda, S., Narita, A. et al. Front Cell Dev Biol (2023) 11:1105460-1105460. DOI 10.3389/fcell.2023.1105460 · PubMed

Other PDB entries of the same protein (UniProt P68032 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8GT1 1.35 Å, Crystal structure of human cardiac alpha actin A108G mutant (ADP-Pi state) in complex…
8GT5 1.4 Å, Crystal structure of human cardiac alpha actin Q137A mutant (ADP-Pi state) in complex…
8GSU 1.5 Å, Crystal structure of human cardiac alpha actin (WT_ADP-Pi) in complex with fragmin F1…
8GT2 1.5 Å, Crystal structure of human cardiac alpha actin P109A mutant (AMPPNP state) in complex…
8GT3 1.5 Å, Crystal structure of human cardiac alpha actin P109A mutant (ADP-Pi state) in complex…
8GT4 1.55 Å, Crystal structure of human cardiac alpha actin Q137A mutant (AMPPNP state) in complex…
9ZBL 2.79 Å, Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex
8ZI9 3.08 Å, Human left ventricle actin and myosin complex
9BPH 3.12 Å, cryo-EM structure of cardiac muscle alpha-actin M305L hcm mutant
9ZBP 3.12 Å, Helical Reconstruction of the Complex of Pseudo-Acetylated Human Cardiac Actin…
8ZB7 3.19 Å, Human left ventricle ATM complex
9B3R 3.5 Å, The structure of human cardiac F-actin

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