8HAO: Human parathyroid hormone receptor-1 dimer

Human parathyroid hormone receptor-1 dimer. Determined by electron microscopy at 3.76 Å resolution. Released 21 Dec 2022.

Method: Electron microscopy
Resolution: 3.76 Å
Organisms: Bos taurus, Rattus norvegicus, Lama glama
Chains: 12
Atoms: 18,479
Mol. weight: 334.84 kDa
Released: 21 Dec 2022

Explore 8HAO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HAO contains 63 α-helices and 100 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-32	27
β-strand	34-40	7	1
α-helix	46-53	8
β-strand	184-191	8	1
β-strand	194-201	8	1
α-helix	213-215	3
β-strand	220-226	7	1
α-helix	233-245	13
β-strand	253-259	7	1
α-helix	261-270	10
α-helix	275-278	4
α-helix	280-282	3
α-helix	299-319	21
β-strand	326-330	5	1
α-helix	338-357	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	45-52	8	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
α-helix	145	1
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	259-265	7	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-341	7	2

Chain C: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-32	27
β-strand	34-39	6	9
α-helix	46-53	8
β-strand	184-191	8	9
β-strand	194-201	8	9
α-helix	213-215	3
β-strand	220-226	7	9
α-helix	233-245	13
β-strand	253-259	7	9
α-helix	261-270	10
α-helix	275-278	4
α-helix	280-284	5
α-helix	299-319	21
β-strand	326-330	5	9
α-helix	338-357	20

Chain D: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	45-52	8	10
β-strand	58-63	6	11
β-strand	69-74	6	11
β-strand	78-83	6	11
β-strand	89-94	6	11
β-strand	100-105	6	12
β-strand	111-116	6	12
β-strand	121-125	5	12
β-strand	135-139	5	12
β-strand	146-151	6	13
β-strand	156-161	6	13
β-strand	166-170	5	13
β-strand	175-180	6	13
β-strand	187-192	6	14
β-strand	198-203	6	14
β-strand	208-212	5	14
β-strand	218-222	5	14
β-strand	229-234	6	15
β-strand	240-245	6	15
β-strand	249-254	6	15
β-strand	259-265	7	15
β-strand	273-278	6	16
β-strand	284-289	6	16
β-strand	294-298	5	16
β-strand	304-308	5	16
β-strand	315-320	6	10
β-strand	327-331	5	10
β-strand	335-341	7	10

Chains E and G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chains F and N: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	17
β-strand	10-12	3	18
β-strand	17-25	9	17
β-strand	34-39	6	18
β-strand	45-51	7	18
β-strand	58-60	3	18
β-strand	68-73	6	17
β-strand	78-84	7	17
α-helix	88-90	3
β-strand	92-98	7	18
β-strand	118	1	18
β-strand	122-126	5	18

Chains H and P: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-32	30

Chain I: 14 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	34-50	17
β-strand	126	1	19
α-helix	129	1
β-strand	130	1	20
α-helix	131-132	2
β-strand	143	1	20
β-strand	147	1	19
β-strand	149	1	21
β-strand	153	1	21
α-helix	178-211	34
α-helix	218-245	28
α-helix	279-311	33
α-helix	317-346	30
α-helix	358-360	3
α-helix	361-391	31
α-helix	400-417	18
α-helix	419-424	6
α-helix	435-456	22
α-helix	457-461	5
α-helix	464-480	17

1 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha	A, C	protein	361	Bos taurus
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B, D	protein	400	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E, G	protein	71	Bos taurus	P63212 (AlphaFold model)
Nanobody 35	F, N	protein	140	Lama glama
Parathyroid hormone	H, P	protein	35	synthetic construct	P01270 (AlphaFold model)
Parathyroid hormone/parathyroid hormone-related peptide receptor	I, R	protein	476	Homo sapiens	Q03431 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>8HAO_1 Guanine nucleotide-binding protein G(s) subunit alpha (chains A, C)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B, D), FASTA

>8HAO_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B, D)
MHHHHHHSSGLVPRGSHMASHHHHHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACA
DATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYT
TNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYL
SCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDA
SAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSH
DNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMA
VATGSWDSFLKIWNGSSGGGGSGGGGSSGVSGWRLFKKIS

Sequence of entity 3 (E, G), FASTA

>8HAO_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E, G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (F, N), FASTA

>8HAO_4 Nanobody 35 (chains F, N)
MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSHHHHHHEPEA

Sequence of entity 5 (H, P), FASTA

>8HAO_5 Parathyroid hormone (chains H, P)
SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFX

Sequence of entity 6 (I, R), FASTA

>8HAO_6 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains I, R)
DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGK
LYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYR
RCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLAMIYTVGYSVSLASLTVAV
LILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQ
APPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTV
FGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLA
TKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEML
FNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFRRKARSGSSSYSYGPMVSH

Primary citation

Molecular recognition of two endogenous hormones by the human parathyroid hormone receptor-1. Zhao, L.H., Yuan, Q.N., Dai, A.T. et al. Acta Pharmacol Sin (2023) 44:1227-1237. DOI 10.1038/s41401-022-01032-z · PubMed

Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF

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