8HG0: Angiotensin-converting enzyme

Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with white-tailed deer ACE2. Determined by electron microscopy at 3.51 Å resolution. Released 30 Aug 2023.

Method
Electron microscopy
Resolution
3.51 Å
Organisms
Odocoileus virginianus texanus, Severe acute respiratory syndrome coronavirus 2
Chains
2
Atoms
6,470
Mol. weight
94.02 kDa
Ligands
ZN, NAG
Released
30 Aug 2023

Explore 8HG0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HG0 contains 44 α-helices and 17 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 7 β-strands

ElementResiduesLengthSheet
α-helix22-5332
α-helix57-8125
α-helix92-10211
α-helix106-1083
α-helix111-12919
β-strand132-13321
β-strand142-14321
α-helix144-1485
α-helix149-1546
α-helix158-16811
α-helix169-1735
α-helix174-19320
α-helix199-2035
α-helix205-2073
α-helix219-2224
α-helix226-25126
α-helix2611
β-strand262-26322
α-helix264-2663
α-helix276-2783
α-helix289-2913
α-helix294-2974
α-helix299-3013
α-helix304-31714
α-helix325-3284
β-strand33213
β-strand347-35263
β-strand355-35953
α-helix366-38419
α-helix390-3923
α-helix400-41314
α-helix415-4206
α-helix428-4303
α-helix432-44615
α-helix449-46517
α-helix470-4723
α-helix473-4808
α-helix481-4855
β-strand487-48822
α-helix500-5023
α-helix504-5074
α-helix514-53118
α-helix548-55811
α-helix566-5749
α-helix582-59918
Chain B: 6 helices, 10 β-strands
ElementResiduesLengthSheet
α-helix338-3425
β-strand354-35854
α-helix366-3705
β-strand376-38054
β-strand394-403104
α-helix417-4215
α-helix424-4263
β-strand431-43774
α-helix439-4424
β-strand452-45435
α-helix460-4623
β-strand473-47426
β-strand48516
β-strand488-48926
β-strand492-49435
β-strand507-516104

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Angiotensin-converting enzymeAprotein613Odocoileus virginianus texanusQ58DD0 (AlphaFold model)
Spike protein S1Bprotein195Severe acute respiratory syndrome coronavirus 2P0DTC2 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8HG0_1 Angiotensin-converting enzyme (chains A)
MTGSFWLLLSLVAVTAAQSTTEEQAKTFLEKFNHEAEDLSYQSSLASWNYNTNITDENVQ
KMNEARAKWSAFYEEQSRMAKTYSLEEIQNLTLKRQLKALQQSGTSVLSAEKSKRLNTIL
NTMSTIYSTGKVLDPNTQECLALEPGLDDIMENSRDYNRRLWAWEGWRAEVGKQLRPLYE
EYVVLENEMARANNYEDYGDYWRGDYEVTEAGDYDYSRDQLMKDVENTFAEIKPLYEQLH
AYVRAKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFKHKPSIDVTEKMKNQS
WDAERIFKEAEKFFVSISLPHMTQGFWDNSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMC
TKVTMDDFLTAHHEMGHIQYDMAYAAQPYLLRDGANEGFHEAVGEIMSLSAATPHYLKAL
GLLEPDFYEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKEQWMEKWWEMK
REIVGVVEPLPHDETYCDPACLFHVAEDYSFIRYYTRTIYQFQFHEALCKTANHEGALFK
CDISNSTEAGQRLLQMLSLGKSEPWTLALESIVGIKTMDVKPLLNYFEPLFTWLKEQNRN
SFVGWSTEWTPYS
Sequence of entity 2 (B), FASTA
>8HG0_2 Spike protein S1 (chains B)
TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCF
TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYL
YRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVV
LSFELLHAPATVCGP

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn1
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O63

Primary citation

Structural basis of white-tailed deer, Odocoileus virginianus , ACE2 recognizing all the SARS-CoV-2 variants of concern with high affinity. Han, P., Meng, Y., Zhang, D. et al. J Virol (2023) 97:e0050523-e0050523. DOI 10.1128/jvi.00505-23 · PubMed

Other PDB entries of the same protein (UniProt Q58DD0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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