8HQC: GPCR-G protein

Structure of a GPCR-G protein in complex with a natural peptide agonist. Determined by electron microscopy at 3.89 Å resolution. Released 18 Oct 2023.

Method: Electron microscopy
Resolution: 3.89 Å
Organisms: Mus musculus, Homo sapiens
Chains: 6
Atoms: 8,538
Mol. weight: 154.01 kDa
Released: 18 Oct 2023

Explore 8HQC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8HQC contains 46 α-helices and 60 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	36-40	5
α-helix	41-61	21
α-helix	64-66	3
α-helix	70-72	3
α-helix	73-95	23
α-helix	107-110	4
α-helix	112-139	28
α-helix	143-146	4
α-helix	150-174	25
α-helix	203-208	6
α-helix	209-213	5
α-helix	214-233	20
α-helix	240-268	29
α-helix	276-288	13
α-helix	290-302	13
α-helix	309-314	6

Chain B: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	10-30	21
β-strand	34-39	6	1
α-helix	46-52	7
β-strand	186-192	7	1
β-strand	195-201	7	1
α-helix	209-212	4
α-helix	213-216	4
β-strand	221-227	7	1
α-helix	247-255	9
α-helix	259-261	3
β-strand	264-270	7	1
α-helix	272-281	10
α-helix	284-286	3
α-helix	297-310	14
β-strand	319-323	5	1
α-helix	331-350	20

Chain C: 5 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	43	1	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
α-helix	272	1
β-strand	273-278	6	2
β-strand	284-289	6	2
β-strand	294-298	5	2
β-strand	306-307	2	2
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain D: 5 helices, 0 β-strands

Element	Residues	Length
α-helix	2-10	9
α-helix	16-25	10
α-helix	34-37	4
α-helix	38-40	3
α-helix	45-62	18

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-44	15
α-helix	45-47	3
α-helix	54-55	2

Chain H: 6 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-12	3	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	33-39	7	10
β-strand	45-51	7	10
α-helix	53-55	3
β-strand	58-60	3	10
β-strand	65	1	9
β-strand	68-73	6	9
α-helix	74-76	3
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-99	8	10
β-strand	111	1	10
β-strand	115-119	5	10
β-strand	140-142	3	11
β-strand	146-148	3	12
β-strand	155-161	7	11
β-strand	166	1	13
β-strand	172	1	13
β-strand	174-179	6	12
β-strand	186-190	5	12
β-strand	194-195	2	12
α-helix	196	1
β-strand	203-208	6	11
β-strand	211-216	6	11
α-helix	221-223	3
β-strand	226-231	6	12
β-strand	239	1	12
β-strand	243-246	4	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
C5a anaphylatoxin chemotactic receptor 1	A	protein	407	Mus musculus	P30993 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	B	protein	240	Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	350	Homo sapiens	P62873 (AlphaFold model)
C5a anaphylatoxin	D	protein	74	Homo sapiens	P01031 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
Antibody fragment	H	protein	256	Mus musculus

Sequence of entity 1 (A), FASTA

>8HQC_1 C5a anaphylatoxin chemotactic receptor 1 (chains A)
MGKTIIALSYIFCLVFADYKDDDDAANFTPVNGSSGNQSVRLVTSSSLEVLFQGPGSDPI
DNSSFEINYDHYGTMDPNIPADGIHLPKRQPGDVAALIIYSVVFLVGVPGNALVVWVTAF
EARRAVNAIWFLNLAVADLLSCLALPVLFTTVLNHNYWYFDATACIVLPSLILLNMYASI
LLLATISADRFLLVFKPIWCQKVRGTGLAWMACGVAWVLALLLTIPSFVYREAYKDFYSE
HTVCGINYGGGSFPKEKAVAILRLMVGFVLPLLTLNICYTFLLLRTWSRKATRSTKTLKV
VMAVVICFFIFWLPYQVTGVMIAWLPPSSPTLKRVEKLNSLCVSLAYINCCVNPIIYVMA
GQGFHGRLLRSLPSIIRNALSEDSVGRDSKTFTPSTTDTSTRKSQAV

Sequence of entity 2 (B), FASTA

>8HQC_2 Guanine nucleotide-binding protein G(o) subunit alpha (chains B)
MGHHHHHHENLYFQGTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGADNSGKST
IVKQMKIIHGGSGGSGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIF
CVDLSDYNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGP
NTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIIIANNLRGCGLY

Sequence of entity 3 (C), FASTA

>8HQC_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (D), FASTA

>8HQC_4 C5a anaphylatoxin (chains D)
TLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQ
LRANISHKDMQLGR

Sequence of entity 5 (G), FASTA

>8HQC_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (H), FASTA

>8HQC_6 Antibody fragment (chains H)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQ

Primary citation

Molecular basis of anaphylatoxin binding, activation, and signaling bias at complement receptors. Yadav, M.K., Maharana, J., Yadav, R. et al. Cell (2023) 186:4956-4973.e21. DOI 10.1016/j.cell.2023.09.020 · PubMed

Other PDB entries of the same protein (UniProt P30993 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9KYU 2.72 Å, Structure of beta-arrestin1 in complex with mouse C5aR1pp
9KY2 2.78 Å, Structure of beta-arrestin2 in complex with mouse C5aR1pp
9KX6 3.11 Å, Structure of human C5a-desArg bound mouse C5aR1 in complex with Go
9KWX 3.13 Å, Structure of mouse C5a-desArg bound mouse C5aR1 in complex with Go
9KWG 3.15 Å, Structure of mouse C5a bound mouse C5aR1 in complex with Go
9KXS 3.31 Å, Structure of EP67 bound mouse C5aR1 in complex with Go
8HPT 3.39 Å, Structure of C5a-pep bound mouse C5aR1 in complex with Go

Browse structure collections

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