8I9Z: Brix domain-containing protein

Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Spb4. Determined by electron microscopy at 2.7 Å resolution. Released 17 May 2023.

Method: Electron microscopy
Resolution: 2.7 Å
Organism: Chaetomium thermophilum
Chains: 60
Atoms: 167,255
Mol. weight: 3468.45 kDa
Ligands: GTP, ZN, ADP, MG
Released: 17 May 2023

Explore 8I9Z in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8I9Z contains 617 α-helices and 524 β-strands across 58 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain CA: 8 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	26	1	5
β-strand	36-41	6	6
α-helix	46-58	13
β-strand	62-68	7	6
α-helix	69	1
α-helix	76-83	8
β-strand	88-95	8	6
β-strand	99-106	8	6
β-strand	112-121	10	6
β-strand	133	1	7
β-strand	136	1	8
α-helix	137-138	2
β-strand	139-142	4	6
α-helix	145-148	4
α-helix	150-162	13
β-strand	169	1	5
β-strand	173	1	8
β-strand	178-184	7	6
β-strand	189-199	11	6
α-helix	200-202	3
β-strand	228-233	6	6
β-strand	237-246	10	6
β-strand	254-257	4	6
α-helix	264-294	31

Chain CB: 12 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	20-39	20
α-helix	41-42	2
β-strand	47	1	14
α-helix	53-58	6
β-strand	61-68	8	15
β-strand	77-83	7	16
β-strand	97-100	4	17
α-helix	105-113	9
α-helix	118-123	6
β-strand	124-128	5	17
α-helix	130-136	7
α-helix	140-149	10
β-strand	152-156	5	17
α-helix	163-172	10
α-helix	178-179	2
β-strand	180-182	3	17
α-helix	217-229	13
β-strand	231-235	5	16
β-strand	240-247	8	15
α-helix	252-269	18
α-helix	274-276	3
β-strand	278-284	7	15
β-strand	290-293	4	15

Chain CC: 30 helices, 39 β-strands

Element	Residues	Length	Sheet
α-helix	123-125	3
β-strand	137-141	5	21
β-strand	147-151	5	21
α-helix	152-154	3
α-helix	177-180	4
β-strand	185-188	4	22
α-helix	193	1
β-strand	194-196	3	22
α-helix	197	1
α-helix	203-211	9
β-strand	217-221	5	23
β-strand	228-230	3	23
α-helix	233-244	12
α-helix	256-259	4
α-helix	274-277	4
α-helix	278-280	3
α-helix	285-300	16
α-helix	309-315	7
α-helix	334-336	3
α-helix	341-348	8
α-helix	350-352	3
β-strand	353	1	24
α-helix	358-360	3
α-helix	361-363	3
α-helix	364-372	9
α-helix	374	1
α-helix	394-396	3
α-helix	399-411	13
α-helix	414	1
β-strand	415-421	7	25
α-helix	425-428	4
α-helix	430-431	2
α-helix	435-438	4
β-strand	445-449	5	26
β-strand	456-461	6	27
β-strand	467-472	6	27
β-strand	476-481	6	27
β-strand	487-492	6	27
α-helix	498	1
β-strand	499-504	6	28
β-strand	513-517	5	28
β-strand	521-524	4	28
α-helix	533-543	11
β-strand	568-570	3	28
α-helix	574-579	6
β-strand	581-586	6	28
β-strand	593-596	4	29
β-strand	602-606	5	29
α-helix	611-613	3
β-strand	615-619	5	29
β-strand	624-626	3	29
β-strand	638-641	4	30
β-strand	648-651	4	30
β-strand	656-660	5	30
β-strand	665-670	6	30
β-strand	677-682	6	31
β-strand	688-693	6	31
β-strand	697-702	6	31
β-strand	711-714	4	31
β-strand	720-725	6	32
β-strand	732-737	6	32
β-strand	742-748	7	32
α-helix	756-757	2
β-strand	758-765	8	32
β-strand	771	1	33
β-strand	774	1	33
β-strand	776-781	6	26
β-strand	788-792	5	26
β-strand	797-800	4	26

Chain CD: 10 helices, 36 β-strands

Element	Residues	Length	Sheet
β-strand	14-20	7	38
α-helix	30-32	3
β-strand	34-38	5	38
α-helix	43-50	8
β-strand	63-67	5	38
β-strand	70-71	2	38
α-helix	76-82	7
α-helix	89-90	2
β-strand	91-97	7	38
α-helix	98-102	5
β-strand	103-109	7	39
β-strand	114-120	7	40
α-helix	125-131	7
β-strand	145-150	6	40
β-strand	155-159	5	40
β-strand	164-167	4	40
α-helix	171-173	3
β-strand	182-185	4	41
β-strand	190-194	5	41
β-strand	200-209	10	41
β-strand	212-221	10	41
β-strand	228-234	7	42
β-strand	239-244	6	42
β-strand	248-253	6	42
α-helix	264-266	3
β-strand	285-287	3	41
β-strand	291-293	3	42
β-strand	300-305	6	43
β-strand	312-317	6	43
β-strand	321-326	6	43
β-strand	331-337	7	43
β-strand	342-348	7	44
β-strand	357-362	6	44
β-strand	366-370	5	44
β-strand	382-385	4	44
β-strand	391-396	6	45
β-strand	403-408	6	45
β-strand	413-417	5	45
β-strand	422-423	2	44
β-strand	433-435	3	44
β-strand	439	1	45
α-helix	444-449	6
α-helix	454-455	2
β-strand	463-468	6	39
β-strand	473-477	5	39
β-strand	481-486	6	39

Chain CE: 23 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	112	1	51
α-helix	120-129	10
β-strand	133	1	51
α-helix	136-139	4
α-helix	142-147	6
β-strand	151-154	4	52
α-helix	162-175	14
β-strand	185-189	5	53
α-helix	193-206	14
β-strand	215-217	3	53
α-helix	223-232	10
β-strand	236-239	4	53
α-helix	241-249	9
β-strand	260-263	4	53
α-helix	266-272	7
α-helix	275-284	10
β-strand	291-294	4	53
β-strand	295	1	52
α-helix	301-310	10
β-strand	316-319	4	52
β-strand	334-338	5	54
α-helix	341-343	3
α-helix	344-354	11
β-strand	359-364	6	54
α-helix	367-379	13
β-strand	385-386	2	54
α-helix	393-405	13
β-strand	409-414	6	54
α-helix	415-418	4
α-helix	422-423	2
β-strand	428-431	4	54
α-helix	438-450	13
β-strand	457-462	6	54
α-helix	464-466	3
α-helix	468-475	8
β-strand	480-482	3	54
β-strand	483	1	14
α-helix	494-503	10
α-helix	505-523	19
α-helix	538-545	8
α-helix	571-572	2

Chain CF: 14 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	6-10	5
α-helix	20-33	14
β-strand	39-44	6	56
α-helix	53-59	7
β-strand	64-66	3	56
α-helix	70-77	8
α-helix	89-92	4
α-helix	93-95	3
β-strand	100-105	6	56
α-helix	109-118	10
β-strand	121-123	3	57
α-helix	129	1
β-strand	130	1	58
α-helix	131	1
β-strand	135-137	3	59
β-strand	141	1	60
β-strand	143	1	61
α-helix	151-153	3
β-strand	156	1	61
α-helix	157-158	2
α-helix	159-161	3
α-helix	162-168	7
β-strand	173-175	3	60
β-strand	178-180	3	60
β-strand	192-195	4	59
β-strand	200	1	58
α-helix	203-211	9
β-strand	217-219	3	57
β-strand	222-228	7	56
β-strand	234-236	3	56

Chain CG: 10 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	2-3	2	69
β-strand	5	1	70
α-helix	6-19	14
β-strand	20	1	71
α-helix	23-26	4
β-strand	36-40	5	69
β-strand	44-49	6	69
α-helix	50-55	6
β-strand	64-67	4	72
β-strand	69-74	6	69
β-strand	80-82	3	69
α-helix	84-86	3
α-helix	87-92	6
β-strand	94	1	71
β-strand	96-100	5	73
α-helix	102-110	9
α-helix	112-113	2
β-strand	114-115	2	73
α-helix	116-118	3
β-strand	119-121	3	73
β-strand	131-136	6	73
β-strand	141-147	7	73
α-helix	151-154	4
β-strand	162-169	8	73
α-helix	172-175	4

Chain CH: 26 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-9	3
α-helix	13-27	15
α-helix	38-64	27
α-helix	68-69	2
α-helix	75-85	11
α-helix	87-116	30
α-helix	121-140	20
α-helix	143-157	15
β-strand	168-173	6	76
α-helix	179-186	8
β-strand	204-210	7	76
β-strand	213-219	7	76
α-helix	220	1
α-helix	233-244	12
β-strand	248-254	7	76
α-helix	257-259	3
α-helix	263-272	10
α-helix	274-277	4
β-strand	282-287	6	76
α-helix	299-310	12
β-strand	314-318	5	76
α-helix	327-348	22
α-helix	359-368	10
β-strand	371	1	77
α-helix	377-380	4
α-helix	387-389	3
α-helix	390-393	4
α-helix	405-410	6
α-helix	430-432	3
β-strand	440-441	2	78
β-strand	444-445	2	78
α-helix	446-449	4
α-helix	454-470	17
α-helix	484-509	26
β-strand	525-526	2	79
α-helix	527-537	11

50 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
RNA (3341-mer)	C1	RNA	3341	Chaetomium thermophilum
RNA (319-mer)	C2	RNA	319	Chaetomium thermophilum
Brix domain-containing protein	CA	protein	316	Chaetomium thermophilum	G0RXZ3 (AlphaFold model)
Ribosome biogenesis protein C8F11.04	CB	protein	391	Chaetomium thermophilum	G0S7X0 (AlphaFold model)
Ribosome biogenesis protein ERB1	CC	protein	801	Chaetomium thermophilum	G0SCK6 (AlphaFold model)
Ribosome biogenesis protein YTM1	CD	protein	495	Chaetomium thermophilum	G0SFB5 (AlphaFold model)
RNA helicase	CE	protein	598	Chaetomium thermophilum	G0RYU9
Ribosome assembly factor mrt4	CF	protein	270	Chaetomium thermophilum	G0S616
60S ribosome subunit biogenesis protein NIP7	CG	protein	184	Chaetomium thermophilum	G0S435
Nucleolar GTP-binding protein 1	CH	protein	661	Chaetomium thermophilum	G0S8F1
Putative RNA-binding protein	CI	protein	414	Chaetomium thermophilum	G0S1H0
Pescadillo homolog	CJ	protein	679	Chaetomium thermophilum	G0SHX6

47 more molecules are not listed.

Sequence of entity 1 (C1), FASTA

>8I9Z_1 RNA (3341-MER) (chains C1)
GGUUGACCUCGGAUCAGGUAGGAGGACCCGCUGAACUUAAGCAUAUCAAUAAGCGGAGGA
AAAGAAACCAACAGGGAUUGCCCUAGUAACGGCGAGUGAAGCGGCAACAGCUCAAAUUUG
AAAGCUGGCUUCGGCCCGCGUUGUAAUUUGGAGAGGAUGCUUUGGGCGAGGCUCCUUCUG
AGUUCCCUGGAACGGGACGCCACAGAGGGUGAGAGCCCCGUAUAGUUGGAAGCCAAGCCU
GUGUAAAGCUCCUUCGACGAGUCGAGUAGUUUGGGAAUGCUGCUCAAAAUGGGAGGUAAA
UUUCUUCUAAAGCUAAAUACCGGCCAGAGACCGAUAGCGCACAAGUAGAGUGAUCGAAAG
AUGAAAAGCACUUUGAAAAGAGGGUUAAAUAGCACGUGAAAUUGUUGAAAGGGAAGCGCU
UGUGACCAGACUUGCGCCCGGCGGAUCAUCCGGUGUUCUCACCGGUGCACUCCGCCGGGC
UCAGGCCAGCAUCGGUUCUGGCGGGGGGAUAAAGGCCCAGGGAAUGUGGCUCCUCCGGGA
GUGUUAUAGCCCUGGGUGUAAUACCCUCGCCGGGACCGAGGACCGCGCUCUGCAAGGAUG
CUGGCGUAAUGGUCACCAGCGACCCGUCUUGAAACACGGACCAAGGAGUCAAGGUUUUGC
GCGAGUGUUUGGGUGUAAAACCCGCACGCGUAAUGAAAGUGAACGUAGGUGAGAGCUUCG
GCGCAUCAUCGACCGAUCCUGAUGUAUUCGGAUGGAUUUGAGUAGGAGCGUUAAGCCUUG
GACCCGAAAGAUGGUGAACUAUGCUUGGAUAGGGUGAAGCCAGAGGAAACUCUGGUGGAG
GCUCGCAGCGGUUCUGACGUGCAAAUCGAUCGUCAAAUCUGAGCAUGGGGGCGAAAGACU
AAUCGAACCAUCUAGUAGCUGGUUACCGCCGAAGUUUCCCUCAGGAUAGCAGUGUCGACC
UUCAGUUUUAUGAGGUAAAGCGAAUGAUUAGGGACUCGGGGGCGAUUUUUAGCCUUCAUC
CAUUCUCAAACUUUAAAUAUGUAAGAAGCCCUUGUUACUUAACUGAACGUGGGCAUUCGA
AUGUAUCGACACUAGUGGGCCAUUUUUGGUAAGCAGAACUGGCGAUGCGGGAUGAACCGA
ACGCGGGGUUAAGGUGCCGGAGUGGACGCUCAUCAGACACCACAAAAGGCGUUAGUACAU
CUUGACAGCAGGACGGUGGCCAUGGAAGUCGGAAUCCGCUAAGGACUGUGUAACAACUCA
CCUGCCGAAUGUACUAGCCCUGAAAAUGGAUGGCGCUCAAGCGUCCCACCCAUACCCCGC
CCUCAGGGUAGAAACGAUGCCCUGAGGAGUAGGCGGCCGUGGAGGUCAGUGACGAAGCCU
AGGGCGUGAGCCCGGGUCGAACGGCCUCUAGUGCAGAUCUUGGUGGUAGUAGCAAAUACU
UCAAUGAGAACUUGAAGGACCGAAGUGGGGAAAGGUUCCAUGUGAACAGCGGUUGGACAU
GGGUUAGUCGAUCCUAAGCCAUAGGGAAGUUCCGUUUCAAAGGGGCACUCGUGCCCCGUG
UGGCGAAAGGGAAGCCGGUUAAUAUUCCGGCACCUGGAUGUGGGUUUUGCGCGGCAACGC
AACUGAACGCGGAGACGACGGCGGGGGCCCCGGGCAGAGUUCUCUUUUCUUCUUAACGGU
CUAUCACCCUGGAAACAGUUUGUCUGGAGAUAGGGUUUAAUGGCCGGAAGAGCCCGACAC
UUCUGUCGGGUCCGGUGCGCUCUCGACGUCCCUUGAAAAUCCGCGGGAGGGAAUAAUUCU
CACGCCAGGUCGUACUCAUAACCGCAGCAGGUCCCCAAGGUGAACAGCCUCUGGUUGAUA
GAACAAUGUAGAUAAGGGAAGUCGGCAAAAUAGAUCCGUAACUUCGGGAAAAGGAUUGGC
UCUAAGGGUUGGGCACGUUGGGCUUUGGGCGGACGCCCUGGGAGCAGAGGGCCUCUAGCC
GGGCAACCGGCCGGCGGCCCUCAGCACCCGGGGUUGAAGCCCUUAGCAGGCUUCGGCCGU
CCGGCGUGCGGUUAACAACCAACUUAGAACUGGUACGGACAGGGGGAAUCUGACUGUCUA
AUUAAAACAUAGCAUUGCGAUGGCCAGAAAGUGGUGUUGACGCAAUGUGAUUUCUGCCCA
GUGCUCUGAAUGUCAAAGUGAAGAAAUUCAACCAAGCGCGGGUAAACGGCGGGAGUAACU
AUGACUCUCUUAAGGUAGCCAAAUGCCUCGUCAUCUAAUUAGUGACGCGCAUGAAUGGAU
UAACGAGAUUCCCACUGUCCCUAUCUACUAUCUAGCGAAACCACAGCCAAGGGAACGGGC
UUGGCAAAAUCAGCGGGGAAAGAAGACCCUGUUGAGCUUGACUCUAGUUUGACAUUGUGA
AAAGACAUAGGAGGUGUAGAAUAGGUGGGAGCUUCGGCGCCAGUGAAAUACCACUACUCC
UAUUGUUUUUUUACUUAUUCAAUGAAGCGGGGCUGGACUUGCGUCCAACUUCUGGAGUUA
AGGUCCUUCGCGGGCCGACCCGGGUUGAAGACAUUGUCAGGUGGGGAGUUUGGCUGGGGC
GGCACAUCUGUUAAACCAUAACGCAGGUGUCCUAAGGGGGGCUCAUGGAGAACAGAAAUC
UCCAGUAGAACAAAAGGGUAAAAGUCCCCUUGAUUUUGAUUUUCAGUGUGAAUACAAACC
AUGAAAGUGUGGCCUAUCGAUCCUUUAGUCCCUCGAAAUUUGAGGCUAGAGGUGCCAGAA
AAGUUACCACAGGGAUAACUGGCUUGUGGCGGCCAAGCGUUCAUAGCGACGUCGCUUUUU
GAUCCUUCGAUGUCGGCUCUUCCUAUCAUACCGAAGCAGAAUUCGGUAAGCGUUGGAUUG
UUCACCCACUAAUAGGGAACGUGAGCUGGGUUUAGACCGUCGUGAGACAGGUUAGUUUUA
CCCUACUGAUGAACUCGUCGCAAUGGUAAUUCAGCUUAGUACGAGAGGAACCGCUGAUUC
AGAUAAUUGGUUUUUGCGGUUGUCCGACCGGGCAGUGCCGCGAAGCUACCAUCUGCUGGA
UAAUGGCUGAACGCCUCUAAGUCAGAAUCCAUGCCAGAACGCGACGAUACUACCCGCACG
UUGUAGACGUAUAAGAAUAGGCUCCGGCCUCGUAUCCUAGCAGGCGAUUCCUCCGCCGGC
CUCGAAGUGGCCGUCGGUAAUUCGCGUAUUGCAAUUUAGACACGCGCGGGAUCAAAUCCU
UUGCAGACGACUUAGAUGUGCGAAAGGGUCCUGUAAGCAGUAGAGUAGCCUUGUUGUUAC
GAUCUGCUGAGGGUAAGCCCUCCUUCGCCUAGAUUUCCCAG

Sequence of entity 2 (C2), FASTA

>8I9Z_2 RNA (319-MER) (chains C2)
AAACUUUCAACAACGGAUCUCUUGGUUCUGGCAUCGAUGAAGAACGCAGCGAAAUGCGAU
AAGUAAUGUGAAUUGCAGAAUUCCGUGAAUCAUCGAAUCUUUGAACGCACAUUGCGCCCG
CCGGUAUUCCGGCGGGCAUGCCUGUUCGAGCGUCAUUUCAACCAUCAAGCCCUGGGCUUG
UGUUGGGGACCCGCGGCUGCUCGCGGGCCCUGAAAAGCAGUGGCGGGCUCGCUAGUCACA
CCGAGCGUAGUAGAACUUCUAUUCGAUCUCGCUCAGGGCGUGCGGCGGGUGCCAGCCGUA
AAACCCCAGCUUCUUCUAA

Sequence of entity 3 (CA), FASTA

>8I9Z_3 Brix domain-containing protein (chains CA)
MAAVYKTIAKVTGSKEDKDKEDRSNIIAERKNKQRVLVLSSRGVTYRHRHLLNDLASMLP
HGRKDAKFDTKSRLYELCELAELYNCNNVLFFEARKGKDLYMWFSKVPNGPTVKFYAQNL
HTMEELHFQGNCLKGSRPILSFDAAFEQEPYLKVIKELFLHTFGVPQGHKKSKPFIDHVL
SFSVADGKIWVRNYEIREVEKVKGEADKREEGETEKPKAAKPGSKDTDINLIEIGPRFVL
TPIIIQEGSFGGPILYENKRFISPNKIRAELRKAKAARHHARMEQQRDLLARKRQLGLDE
STPKKKDDLDTRELFA

Sequence of entity 4 (CB), FASTA

>8I9Z_4 Ribosome biogenesis protein C8F11.04 (chains CB)
MAPSTAVAKKETDAVIPVDPDQTLKACKALLAHIKKAAAAPRPDGKQNLLADEESTVAET
PIWLTLTTKKHIHDSHRLQPGKIILPHPLNTSEEISVCLITADPQRFYKNAVADEFPEDL
RAKIGRVIDISHLKAKFKAYEAQRKLFSEHDVFLADTRIINRLPKALGKTFYKTTTKRPI
PVVLMAQREKVNGKRVPAPKGKKEKRDPLENANARPIPEIVAEIRKAIGAALVHLSPSTN
TAIKVGYANWEPEKLAANIETVIRELVERFVPQKWQNVRNFYVKGPETAALPIYQTDELW
LDESKVVPDGQEPARALPGKREKANIGKKRKPLEDASQPALEETGKDERPKKKAKKTLPE
SNDDKLDKAIAERKEQLKKQKAAAKKVAADI

Sequence of entity 5 (CC), FASTA

>8I9Z_5 Ribosome biogenesis protein ERB1 (chains CC)
MGSKIVEKKRKSRDSDSESDNELGDGLFDGVLSQSEDEEDYIPSSEVDEDDDDDADESAS
EDSDDSNDSEDDEVEEDDEALLSDEIPSEGESEKDQDLAESKESKQDQDKEPSEPEILEP
FVDPPRKEDEELEDRNYRIEKDANGGIRYVYDEIDPVYDSDDTDYNVPVNTIGNIPLSFY
DSYPHIGYDINGKKIMRPATGDALQNLLDSIEVPEGWTGLTDPNTGKPLNLSRDELELIR
KVQQGLIPDDVEDPYPDTVEWFTSVEEKMPLSAAPEPKRRFIPSKNEAKQIMKLVRAIRE
GRILPYKPPEEREREELEKEEEFYDLWQNEEPQPPNPMHIPAPKLPPPGYDLSYNPPPEY
LPTKEEREEWEKMDPEDREKDYLPTKYDSLRKVPAWGNFVKERFERCMDLYLAPRVRKNR
LNIDPNSLLPKLPSPDELKPFPTVQQTIFRGHEGRVRSVAIDPTGVALATGGDDGTVRVW
ELLTGRQVWSVKLNGDEAVNTVRWRPTKDTFILAAAAGEDIFLMIPTHPSVTPALDQASR
DILNAGFGHATNGKQQANLPPGKEPPGKWARPGTRLEDEGVLLRITVRSTIKAISWHRRG
DHFATVSPSGQRSSVAIHTLSKHLTQIPFRKLNGLAQTASFHPLRPLFFVATQRSIRCYD
LQKLELVKIVQPGAKWISSFDVHPGGDNLVVGSYDKRLLWHDLDLSNRPYKTMRFHTEAI
RAVRFHKGGLPLFADASDDGSLQIFHGKVPNDQLENPTIVPVKMLKGHKVVNKLGVLDID
WHPREPWCVSAGADGTARLWM

Sequence of entity 6 (CD), FASTA

>8I9Z_6 Ribosome biogenesis protein YTM1 (chains CD)
MDAPMEDAPAPVAQVKVIFTTTEPDLELPESKRQLLVPADIRRYGLSRILNSESMLDTGS
IPFDFLINGSFLRSSLEDYLTSNGLSLDTTLTLQYVRSLIPPVYEASFEHDDWVSAVDVL
SATSPAGRWSSAANSSAAVQPGQERVLSASYDGLLRIWNASGSVIATSPSGSHGGHTASI
KAAKFLTSDRLASAGMDRTVRVWKYTESDHFTGELKPTLELYGHTGSVDWLDVDGHSKHI
LTASADGAIGFWSASKASAPEPDASLLPGAHVSKRRKATSSVSTAQRGPLGLWSIHTAPA
TAAIFDPRDRTVAYSASQDHTVRTLDLTTGQVVSTLTLTHPLLSLSALTRAGTTSPLLAA
GTSARHITMVDPRASSATTSVMTLRGHANKVVSLSPSPENEYSLVSGSHDGTCRVWDLRS
VRPATKEEGSLGGVSEPVYVIERESWASKGKKKRPVAGDGCKVFSVVWDKLGIFSGGEDK
KVQVNRGRNIVTEQK

Sequence of entity 7 (CE), FASTA

>8I9Z_7 RNA helicase (chains CE)
MASESSKKRKFKDSKGPKPDEAVAKPSKFKKLKRDPTPEERETSDSESGNSDGQHEPDQV
DSDGEDAVSDDGSFDEKVAHQDEDGSRPENGTDLEDVPKNDNLLPPPTNAQEFSELNLSE
KTTKAIAEMGFTKMTEIQRRAIPPALAGKDVLGAAKTGSGKTLAFLIPAVEMLSSLRFKP
RNGTGAIVVTPTRELALQIFGVARELMKYHSQTYGVVIGGANRRAEAEKLGKGVNLLIAT
PGRLLDHLQNTPFVFKNLKSLIIDEADRILEIGFEDEMRQIVKILPKEDRQTMLFSATQT
TKVEDLARISLRPGPLYINVDEEKKYSTVEGLEQGYVVVEADKRFLLLFSFLKKMAKKKI
IVFFSSCNSVKYYSELLQYIDLPVLDLHGKQKQQKRTNTFFEFCNAKSGTLICTDVAARG
LDIPQVDWIVQFDPPDDPRDYIHRVGRTARGNNGKGRSLLFLQPCELGFLAHLKAAKVPV
VEYDFPKNKILNVQSQLEKLISTNYYLNQSAKEGYRSYIHAYASHSLRSVFDVHKLDLVK
VAKSFGFSTPPRVDITLSASLSRDKKPQGRRAYGSQPRQGGRYKKVGDIPDPQPNTFG

Sequence of entity 8 (CF), FASTA

>8I9Z_8 Ribosome assembly factor mrt4 (chains CF)
MPKSKRARVYHLIQVNKKGREAKERLFSNIRETIPKYQHCFVFSVDNMRNNYLKDVRHEL
NDCRIFFGKTKLMARALGTTPEEEQADGLHRLTRYLTGTVGLLFTNRDPADIESYFSNLS
QVDFARAGTVAPRTVTVPTGIVYSTGGEVPPEHDVPVSHTLEPELRRLGMPVRMIKGKVC
LGDEKGEASEGYTICKEGEVLDSRQTRLLKLFSICLSEFKVSLLGYWNSASGEVTELEAG
KTRPKREGNRRQAMNGDEIDEDQSSDEDSD

Sequence of entity 9 (CG), FASTA

>8I9Z_9 60S ribosome subunit biogenesis protein NIP7 (chains CG)
MRPLTDQEMKIVLDKLANYMTDLKSLIAPLEDGDRYVFRMQKDRVYYVKLSIANIATCVA
RDKLLSLGTCLGKMTKSGKFRLHITALPILAQNARYKIWVKDNGAQPFLYGSNIVKAHVG
RWTEDCPEHSGCVVYNMADIPLGFGVTARSTAEARRLDPTGIVCFRQADCGEYLRDEDTL
FTGA

Sequence of entity 10 (CH), FASTA

>8I9Z_10 Nucleolar GTP-binding protein 1 (chains CH)
MTGWKDIPPVPTAQEFIDIVLSRTQRRLPTQIRPGFKISRIRAFYTRKVKFTQETCSEKF
GAIISSFPVLSDQHPFHRDLMNILYDADHFKVALGQISTAKNLIETISRDYVRLLKYAQS
LYQCKQLKRAALGRMATLIKRLKDPLIYLDQVRQHLARLPDINPTTRTLLVAGFPNVGKS
SFVRSVTRADTPVEPYAFTTKSLFVGHLDYKYLRYQVIDTPGILDHPLEEMNTIEMQSVT
ALAHLRAAVLYFMDISEQCGFSLKAQINLFKSIKPLFANKMVFIVLNKMDIKKFEELDPE
MQQEINDLTKSGEVEILRASCATQEGVQEVKNHVCERLLVERVSQKLKAGTHSNGNIGTR
LQEVMARIHVATPMDGTTRETFIPEAVKNLKKYDKNDPNRRVLARDIEEANGGAGVFNVD
LRKDWILENPEWKYDKIPEIFDGKNVYDYIDPDIDAKLQALEEEEERLEKEGFYDEDDDL
PDEEEEEILQKAEYIREQHALIRNEAKMRKSLKNRAIIPRKAVKKPLSQLEDHLDQLGVD
TEAIGLRARAQTAQSSRGRSLVRSRGTTADPDAMDIDDGAASAKERLARSRSRARSVAAT
NRLQDGVQGTTLRSKAERQAKLAQRKMNRMARQGEADRHIHASMPKHLFSGKRTIGKTDR
R

Sequence of entity 11 (CI), FASTA

>8I9Z_11 Putative RNA-binding protein (chains CI)
MAAELRKKKSKSAVADADAAISKKEKATKVTKATPKAEKRKAPEEASPVAIKKQKADKDA
VVKKAAVAAEDKIAEKPVKSVLKKETSAKAKEAAKPKKKQEKVEEEEEEALENADAAFGS
DEESELDEQTKALMDTLDDGEESDEDESKQVSTFKKGQDVGKIPTPKKAPQKQTNGDSKP
AGDSQPGVMYIARLPHGFYEHELRGYFSQFGEITRLRVVRNKKTGASRHRAFIEFADAEV
ADIAARTMDKYLLFGHILTCKIVPPAQVHPDLFKGANRRFKVVPWNKMAGRQLERPLSES
QWQVKVAKEEQRRAARAEKLKEMGYEFEAPALKVPKAKPVLENGEEEKKAIETAPAPAVE
EKESKEVAKRQAEEEVEEKAPAPKTKKVKEDKPAKEAATISAPKKGKKSKKTKS

Sequence of entity 12 (CJ), FASTA

>8I9Z_12 Pescadillo homolog (chains CJ)
MGKAKKKGKSGAARNYMTRTQAVKKLQLSLPDFRKLCIWKGIYPREPRDRRKVNKSATAS
TTFYYTKDIQYLLHEPLLQKFREQKALEKKISRALGRGDVSNAARLERNANLPEKTGKPR
YTLNHIIRERYPTFQDALRDLDDCLSMLFLFANLPSTTAVPAKMIARCERLCHEFQHYLI
VTHSLRKSFLSIKGIYYQANIQGEDILWLVPYKFNQRIVGDVDFRIMGTFVEFYMTLLGF
VNYRLYTSIGLKYPPKFDQVKDDQGAELAAFSLEGLNLASQNGEQKAITNGEEHGPDPKV
QAEVDKLVAKLREEEQQANGDKTDEKGEENEGDDKPSDAIDKFEPVAPGGDVLPQPSYSS
SDPSQLFANFTFFLSRETPRQPLEFILRAFGCKRIGWDAVLGEGAFTTDESDPRITHQII
DRPVIRAAVSEDGDGEDNQTSQKLAPNGRYPGRIYVQPQWVWDSINDEELKPPELYAPGA
QLPPHLSPFVKPTQGQYDPTKPLEEQQTEAEALEAELEDAQAQQEGSDEESGSEVENDMS
VASDEEDEQEDDFGGFSDEDEEQSDEGSEEGDEEEDDDEEEEATLERQRELEAELAGKAV
SKGKPLDPKVKAKLEAKKALERKKKQEAEELERAKGMLSKKKRKLFEQMQYSNAKKNAED
AKLRAKRRRIEKEMAAKKA

Ligands and cofactors

ID	Name	Formula	Copies
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
ZN	Zinc ion	Zn	2
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1
MG	Magnesium ion	Mg	1

Primary citation

Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis. Lau, B., Huang, Z., Kellner, N. et al. EMBO Rep (2023) 24:e56910-e56910. DOI 10.15252/embr.202356910 · PubMed

Other PDB entries of the same protein (UniProt G0RXZ3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8IA0 2.7 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Puf6
8I9X 2.8 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-1
8I9P 3.0 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16
8I9R 3.1 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State 5S RNP
8I9V 3.1 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-2
8I9W 3.1 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Dbp10-3
8I9Y 3.1 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-2
8I9T 3.6 Å, Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-1

Browse structure collections

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