8IA7: Gastrin/cholecystokinin type B receptor

Structural insights into human brain gut peptide cholecystokinin receptors. Determined by electron microscopy at 3.1 Å resolution. Released 6 Dec 2023.

Method: Electron microscopy
Resolution: 3.1 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 8,586
Mol. weight: 165.31 kDa
Released: 6 Dec 2023

Explore 8IA7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8IA7 contains 30 α-helices and 61 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	15
α-helix	47-55	9
β-strand	183-190	8	15
β-strand	193-200	8	15
α-helix	211-213	3
β-strand	219-225	7	15
α-helix	241-253	13
β-strand	262-268	7	15
α-helix	270-276	7
α-helix	298-308	11
α-helix	312	1
β-strand	318	1	15
β-strand	321	1	15
α-helix	330-349	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
α-helix	95	1
β-strand	100-105	6	3
β-strand	111-116	6	3
β-strand	121-125	5	3
β-strand	134-139	6	3
β-strand	146-151	6	4
β-strand	156-161	6	4
β-strand	166-170	5	4
β-strand	176-180	5	4
β-strand	187-192	6	5
β-strand	198-203	6	5
β-strand	207-212	6	5
β-strand	218-222	5	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	284-289	6	7
β-strand	294-298	5	7
β-strand	304-308	5	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	13-23	11
α-helix	30-43	14
α-helix	53-55	3

Chain E: 2 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	8
β-strand	10-12	3	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	33-39	7	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	68-73	6	8
β-strand	78-83	6	8
β-strand	93	1	11
β-strand	95-99	5	10
β-strand	110-111	2	10
β-strand	113	1	8
β-strand	116	1	11
β-strand	117-119	3	9
β-strand	129	1	12
β-strand	134-136	3	13
β-strand	143-148	6	12
β-strand	154	1	14
β-strand	160	1	14
β-strand	162-167	6	13
β-strand	174-178	5	13
β-strand	182-183	2	13
α-helix	184	1
β-strand	191-196	6	12
β-strand	199-204	6	12
β-strand	214-219	6	13
β-strand	231-234	4	13

Chain R: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	55-81	27
α-helix	88-107	20
α-helix	109-116	8
α-helix	123-157	35
α-helix	159-163	5
α-helix	168-185	18
α-helix	188-191	4
α-helix	215-223	9
α-helix	224-228	5
α-helix	229-247	19
α-helix	325-359	35
α-helix	361-366	6
α-helix	371-390	20
α-helix	397-402	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Gastrin/cholecystokinin type B receptor	R	protein	447	Homo sapiens	P32239 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	70	Homo sapiens	P59768 (AlphaFold model)
scfv16	E	protein	269	Mus musculus
Guanine nucleotide-binding protein G(q) subunit alpha	A	protein	353	Homo sapiens	P50148 (AlphaFold model)
CCK-8	L	protein	9	Homo sapiens	P06307

Sequence of entity 1 (R), FASTA

>8IA7_1 Gastrin/cholecystokinin type B receptor (chains R)
MELLKLNRSVQGTGPGPGASLCRPGAPLLNSSSVGNLSCEPPRIRGAGTRELELAIRITL
YAVIFLMSVGGNMLIIVVLGLSRRLRTVTNAFLLSLAVSDLLLAVACMPFTLLPNLMGTF
IFGTVICKAVSYLMGVSVSVSTLSLVAIALERYSAICRPLQARVWQTRSHAARVIVATWL
LSGLLMVPYPVYTVVQPVGPRVLQCVHRWPSARVRQTWSVLLLLLLFFIPGVVMAVAYGL
ISRELYLGLRFDGDSDSDSQSRVRNQGGLPGAVHQNGRCRPETGAVGEDSDGCYVQLPRS
RPALELTALTAPGPGSGSRPTQAKLLAKKRVVRMLLVIVVLFFLCWLPVYSANTWRAFDG
PGAHRALSGAPISFIHLLSYASACVNPLVYCFMHRRFRQACLETCARCCPRPPRARPRAL
PDEDPPTPSIASLSRLSYTTISTLGPG

Sequence of entity 2 (B), FASTA

>8IA7_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>8IA7_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL

Sequence of entity 4 (E), FASTA

>8IA7_4 scfv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAAHHHHHHHH

Sequence of entity 5 (A), FASTA

>8IA7_5 Guanine nucleotide-binding protein G(q) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 6 (L), FASTA

>8IA7_6 CCK-8 (chains L)
DYMGWMDFX

Primary citation

Structural insights into human brain-gut peptide cholecystokinin receptors. Ding, Y., Zhang, H., Liao, Y.Y. et al. Cell Discov (2022) 8:55-55. DOI 10.1038/s41421-022-00420-3 · PubMed

Other PDB entries of the same protein (UniProt P32239 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7F8W 3.1 Å, Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gq
7XOW 3.1 Å, Structural insights into human brain gut peptide cholecystokinin receptors
7F8V 3.3 Å, Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gi
1L4T Solution NMR structure of the CCK2E3

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