8JHB: FZD6 Gs complex

FZD6 Gs complex. Determined by electron microscopy at 3.3 Å resolution. Released 17 Jan 2024.

Method: Electron microscopy
Resolution: 3.3 Å
Organism: Homo sapiens
Chains: 5
Atoms: 8,648
Mol. weight: 153.71 kDa
Released: 17 Jan 2024

Explore 8JHB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JHB contains 31 α-helices and 55 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	3-32	30
β-strand	36-41	6	1
β-strand	207-214	8	1
β-strand	217-224	8	1
α-helix	236-238	3
β-strand	243-249	7	1
α-helix	265-277	13
β-strand	286-292	7	1
α-helix	294-303	10
α-helix	308-311	4
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-349	18
β-strand	359-363	5	1
α-helix	371-390	20

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-25	21
α-helix	38-40	3
β-strand	47-51	5	2
β-strand	58-62	5	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	135-140	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-181	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	293-298	6	8
β-strand	304-309	6	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	32-47	16
α-helix	54-67	14

Chain N: 1 helix, 12 β-strands

Element	Residues	Length	Sheet
β-strand	26-30	5	9
β-strand	34	1	10
β-strand	41-48	8	9
β-strand	57-62	6	11
β-strand	68-74	7	11
β-strand	81-83	3	11
α-helix	85-87	3
β-strand	92-96	5	9
β-strand	101-106	6	9
β-strand	115-121	7	11
β-strand	141	1	11
β-strand	145-147	3	11
β-strand	148	1	10

Chain R: 17 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	174-175	2	12
β-strand	178-179	2	12
β-strand	184	1	13
α-helix	194-213	20
α-helix	214-216	3
α-helix	232-254	23
β-strand	261	1	13
β-strand	272	1	13
β-strand	275	1	14
α-helix	282-286	5
α-helix	287-291	5
α-helix	292-311	20
α-helix	320-323	4
α-helix	324-331	8
α-helix	334-338	5
α-helix	341-344	4
β-strand	349	1	14
β-strand	351	1	15
β-strand	356	1	15
α-helix	372-399	28
α-helix	409-422	14
α-helix	425-457	33
α-helix	460-462	3
α-helix	473-482	10
α-helix	483-485	3
α-helix	489-493	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	A	protein	270	Homo sapiens	Q5JWF2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	74	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	135	Homo sapiens
Frizzled-6	R	protein	537	Homo sapiens	O60353 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8JHB_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas (chains A)
HHHHHHENLYFQGSKTEDKRAQKRAEKKRSKLIDKQLQDEKMGYMCTHRLLLLGADNSGK
STIVKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAI
IFVVDSSDYGSGGSGAGSANRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAG
KSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFT
CAVDTENARRIFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>8JHB_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>8JHB_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MHHHHHHNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPAS
ENPFREKKFFCAIL

Sequence of entity 4 (N), FASTA

>8JHB_4 Nb35 (chains N)
MQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASIS
YTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRG
QGTQVTVSSHHHHHH

Sequence of entity 5 (R), FASTA

>8JHB_5 Frizzled-6 (chains R)
MKTIIALSYIFCLVFADYKDDDDKHHHHHHHSLFTCEPITVPRCMKMAYNMTFFPNLMGH
YDQSIAAVEMEHFLPLANLECSPNIETFLCKAFVPTCIEQIHVVPPCRKLCEKVYSDCKK
LIDTFGIRWPEELECDRLQYCDETVPVTFDPHTEFLGPQKKTEQVQRDIGFWCPRHLKTS
GGQGYKFLGIDQCAPPCPNMYFKSDELEFAKSFIGTVSIFCLCATLFTFLTFLIDVRRFR
YPERPIIYYSVCYSIVSLMYFIGFLLGDSTACNKADEKLELGDTVVLGSQNKACTVLFML
LYFFTMAGTVWWVILTITWFLAAGRKWSCEAIEQKAVWFHAVAWGTPGFLTVMLLAMNKV
EGDNISGVCFVGLYDLDASRYFVLLPLCLCVFVGLSLLLAGIISLNHVRQVIQHDGRNQE
KLKKFMIRIGVFSGLYLVPLVTLLGCYVYEQVNRITWEITWVSDHCRQYHIPCPYQAKAK
ARPELALFMIKYLMTLIVGISAVFWVGSKKTCTEWAGFFKRNRKRDPISESRRVLQE

Primary citation

A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways. Zhang, Z., Lin, X., Wei, L. et al. Cell Discov (2024) 10:3-3. DOI 10.1038/s41421-023-00627-y · PubMed

Other PDB entries of the same protein (UniProt Q5JWF2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9BKK 2.51 Å, Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
8WW2 2.79 Å, GPR3/Gs complex
8I2G 2.8 Å, FSHR-Follicle stimulating hormone-compound 716340-Gs complex
8JLQ 2.84 Å, Fenoldopam-bound hTAAR1-Gs protein complex
9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
9YGZ 3.04 Å, Cryo-EM structure of active mutant human green cone opsin (E129Q) in complex with…
9WNF 3.13 Å, 5HT2AR-miniGq heterotrimer in complex with a selective agonist IHCH-2330
8ZH8 3.19 Å, Human GPR103 -Gq complex bound to QRFP26
8JHI 3.2 Å, FZD3-Gs complex
9WND 3.2 Å, 5HT2AR-miniGq heterotrimer in complex with a psychedelic psilocin
8JLP 3.23 Å, Ralmitaront(RO-6889450)-bound hTAAR1-Gs protein complex
8GY7 3.3 Å, Cryo-EM structure of ACTH-bound melanocortin-2 receptor in complex with MRAP1 and Gs…

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