8JPF: NTS

Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes. Determined by electron microscopy at 3.02 Å resolution. Released 9 Aug 2023.

Method: Electron microscopy
Resolution: 3.02 Å
Organisms: synthetic construct, Homo sapiens
Chains: 2
Atoms: 2,269
Mol. weight: 47.58 kDa
Ligands: SRW
Released: 9 Aug 2023

Explore 8JPF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8JPF contains 14 α-helices and 2 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain R: 14 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	52-54	3
α-helix	60-87	28
α-helix	101-122	22
α-helix	123-127	5
α-helix	137-171	35
α-helix	173-179	7
α-helix	182-199	18
α-helix	202-206	5
β-strand	209-211	3	1
β-strand	222-224	3	1
α-helix	230-241	12
α-helix	242-246	5
α-helix	247-270	24
α-helix	294-328	35
α-helix	336-367	32
α-helix	369-377	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
NTS	L	protein	6	synthetic construct
Neurotensin receptor type 1	R	protein	418	Homo sapiens	P30989 (AlphaFold model)

Sequence of entity 1 (L), FASTA

>8JPF_1 NTS (chains L)
RRPYIL

Sequence of entity 2 (R), FASTA

>8JPF_2 Neurotensin receptor type 1 (chains R)
MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDI
YSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAM
PVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLM
SRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVN
TFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRH
GVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTIN
PILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY

Ligands and cofactors

ID	Name	Formula	Copies
SRW	2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-y…	C26 H31 F N4 O2	1

Primary citation

GPCR activation and GRK2 assembly by a biased intracellular agonist. Duan, J., Liu, H., Zhao, F. et al. Nature (2023) 620:676-681. DOI 10.1038/s41586-023-06395-9 · PubMed

Other PDB entries of the same protein (UniProt P30989 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
9YCX 2.1 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the…
9YCY 2.2 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Canonical…
9VAW 2.24 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in…
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…
20ZH 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…
7UL2 2.4 Å, CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
9P82 2.4 Å, NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Canonical, AHD Closed State (C-Closed-GTP)
20ZC 2.6 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gi1 (delipidated) complex in…
9P81 2.6 Å, NTSR1-Gi-NTS(8-13), GTP-bound Complex in the Canonical, AHD Open State (C-Open-GTP)
8ZYT 2.65 Å, Cryo-EM structure of neurotensin receptor 1 in complex with beta-arrestin1 and SBI-553…

Browse structure collections

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