8OR2: CAND1-CUL1-RBX1-DCNL1

CAND1-CUL1-RBX1-DCNL1. Determined by electron microscopy at 3.2 Å resolution. Released 28 Jun 2023.

Method
Electron microscopy
Resolution
3.2 Å
Organism
Homo sapiens
Chains
4
Atoms
16,558
Mol. weight
273.88 kDa
Ligands
ZN
Released
28 Jun 2023

Explore 8OR2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8OR2 contains 114 α-helices and 20 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 33 helices, 9 β-strands

ElementResiduesLengthSheet
α-helix25-328
α-helix39-5315
α-helix87-10620
α-helix116-13621
α-helix138-1414
α-helix159-17214
α-helix175-19016
α-helix198-21013
α-helix226-2283
α-helix229-2335
α-helix234-25421
α-helix257-27822
α-helix283-29513
α-helix297-2993
α-helix300-31213
α-helix316-32712
α-helix334-35421
α-helix365-38420
α-helix389-40416
α-helix407-4126
α-helix417-43014
β-strand43111
α-helix443-45311
α-helix460-47516
β-strand47911
α-helix482-49514
α-helix499-52426
β-strand536-54162
α-helix548-5525
α-helix560-57112
β-strand577-592162
β-strand599-60462
α-helix605-6117
α-helix622-6298
α-helix633-64513
β-strand681-68332
α-helix691-72131
β-strand724-72633
α-helix727-73711
α-helix746-75813
β-strand762-76433
β-strand771-77443
Chain B: 4 helices, 5 β-strands
ElementResiduesLengthSheet
β-strand23-35132
α-helix36-405
α-helix52-532
α-helix54-585
β-strand70-7124
β-strand79-8024
α-helix81-9010
β-strand93-9425
β-strand99-10025
Chain C: 69 helices, 4 β-strands
ElementResiduesLengthSheet
α-helix5-1410
α-helix20-223
α-helix25-3511
α-helix43-5614
α-helix62-7716
α-helix81-9515
α-helix100-11617
α-helix128-14114
α-helix149-16416
α-helix170-1723
α-helix173-1808
α-helix181-1855
α-helix189-20517
α-helix212-22514
α-helix233-24412
α-helix250-2523
α-helix257-2637
α-helix271-28414
α-helix291-30313
α-helix347-36216
α-helix368-3736
α-helix376-3827
α-helix388-40518
α-helix424-44219
α-helix448-46417
α-helix473-48513
α-helix491-50616
α-helix510-5123
α-helix514-5163
α-helix517-52812
α-helix533-55018
α-helix562-5643
α-helix565-57612
α-helix583-59917
α-helix607-62014
α-helix625-63612
α-helix645-6473
α-helix652-6565
α-helix657-6593
α-helix664-68017
α-helix687-6948
β-strand70216
β-strand70516
α-helix707-72216
α-helix734-7429
α-helix750-76516
α-helix772-7798
α-helix793-80917
α-helix814-82512
α-helix832-84514
α-helix857-8659
α-helix871-88616
α-helix888-90114
α-helix906-91914
α-helix922-9254
α-helix930-94011
α-helix945-95814
α-helix963-97614
α-helix979-99012
α-helix1001-101313
α-helix1020-103617
α-helix1045-105612
β-strand1063-106647
β-strand1073-107647
α-helix1079-109113
α-helix1103-111210
α-helix1117-113014
α-helix1135-11395
α-helix1142-115312
α-helix1157-11582
α-helix1163-118422
α-helix1202-12043
α-helix1206-12094
Chain F: 8 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix66-727
α-helix100-1089
α-helix134-14916
α-helix153-16513
β-strand17318
α-helix175-18511
α-helix193-1986
β-strand20818
α-helix210-22213
α-helix240-25011

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Cullin-1Aprotein813Homo sapiensQ13616 (AlphaFold model)
E3 ubiquitin-protein ligase RBX1Bprotein108Homo sapiensP62877 (AlphaFold model)
Cullin-associated NEDD8-dissociated protein 1Cprotein1238Homo sapiensQ86VP6 (AlphaFold model)
DCN1-like protein 1Fprotein261Homo sapiensQ96GG9 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8OR2_1 Cullin-1 (chains A)
MWSHPQFEKGSAGSAAGSGAGWSHPQFEKLEVLFQGPMSSTRSQNPHGLKQIGLDQIWDD
LRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQF
VGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRH
WVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISG
VVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKK
AEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMY
NLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMS
AFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELE
DTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSK
LQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSY
QRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQ
QLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVN
INVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFK
PRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA
Sequence of entity 2 (B), FASTA
>8OR2_2 E3 ubiquitin-protein ligase RBX1 (chains B)
MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
ASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
Sequence of entity 3 (C), FASTA
>8OR2_3 Cullin-associated NEDD8-dissociated protein 1 (chains C)
GSPEFPGRMASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKM
ILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKT
VIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNF
HPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTR
TYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYP
HVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRA
AAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWL
CDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQH
IPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPF
YKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISC
MGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGV
PILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMA
ISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGY
MDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTD
SIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYL
PFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVA
ECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFL
KTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFK
HTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLST
LCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKS
PLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS
Sequence of entity 4 (F), FASTA
>8OR2_4 DCN1-like protein 1 (chains F)
GSMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESVKG
SLDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFS
KQEFMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMA
IAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDMSNYDEEGAWPV
LIDDFVEFARPQIAGTKSTTV

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn3

Primary citation

Structural and mechanistic insights into the CAND1-mediated SCF substrate receptor exchange. Shaaban, M., Clapperton, J.A., Ding, S. et al. Mol Cell (2023) 83:2332. DOI 10.1016/j.molcel.2023.05.034 · PubMed

Other PDB entries of the same protein (UniProt Q13616 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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