8OXO: Cellular tumor antigen p53

ATM(Q2971A) dimeric C-terminal region activated by oxidative stress in complex with Mg AMP-PNP and p53 peptide. Determined by electron microscopy at 3.0 Å resolution. Released 27 Sept 2023.

Method
Electron microscopy
Resolution
3.0 Å
Organism
Homo sapiens
Chains
4
Atoms
23,986
Mol. weight
733.93 kDa
Ligands
ANP, MG, ZN
Released
27 Sept 2023

Explore 8OXO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8OXO contains 183 α-helices and 30 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 91 helices, 15 β-strands

ElementResiduesLengthSheet
α-helix1464-147714
α-helix1491-150818
α-helix1518-15236
α-helix1525-15284
α-helix1532-154716
α-helix1553-15608
α-helix1569-15713
α-helix1572-158211
α-helix1590-160314
α-helix1608-16103
α-helix1611-162313
α-helix1625-16273
α-helix1628-16347
α-helix1639-16413
α-helix1643-165715
α-helix1658-16603
α-helix1664-167613
β-strand168711
α-helix1694-17029
α-helix1706-172116
α-helix1727-174115
α-helix1744-175310
α-helix1760-17645
α-helix1765-17673
α-helix1786-17883
α-helix1792-17954
α-helix1802-181514
α-helix1828-18314
α-helix1835-185117
α-helix1857-187317
α-helix1903-191715
α-helix1920-19212
α-helix1927-19304
α-helix1938-194710
α-helix1951-197323
α-helix1986-199611
α-helix2001-201111
α-helix2029-203810
α-helix2042-205110
α-helix2057-207014
α-helix2075-208713
α-helix2093-210513
α-helix2124-213613
α-helix2141-216020
β-strand216511
α-helix2169-218820
α-helix2195-221117
α-helix2217-223620
α-helix2242-226322
α-helix2269-228113
α-helix2290-230112
α-helix2305-232117
α-helix2328-234821
α-helix2353-23564
α-helix2357-23615
α-helix2362-23709
α-helix2377-240630
α-helix2408-242922
α-helix2436-247540
α-helix2481-24833
α-helix2484-249310
α-helix2498-25069
α-helix2508-25103
α-helix2513-25153
α-helix2520-25245
α-helix2535-255117
α-helix2553-25553
α-helix2557-25648
α-helix2568-25725
α-helix2593-261220
α-helix2614-263219
α-helix2636-26383
β-strand2645-264622
α-helix2647-26482
α-helix2652-26554
β-strand266313
β-strand268213
β-strand2683-268644
β-strand2689-269022
β-strand2691-269224
β-strand2700-270674
β-strand2711-271884
α-helix2723-274119
α-helix2743-27486
β-strand2757-275934
β-strand2764-276854
α-helix2769-27702
β-strand2773-277535
α-helix2776-27805
α-helix2787-27915
α-helix2796-27972
α-helix2798-280811
α-helix2813-282513
α-helix2831-28333
α-helix2834-28385
α-helix2842-286625
α-helix2873-28753
β-strand2876-287945
β-strand2885-288735
α-helix2912-29176
α-helix2926-293914
α-helix2942-29498
α-helix3004-301815
α-helix3028-304013
α-helix3042-30465
α-helix3050-30523
Chain B: 92 helices, 15 β-strands
ElementResiduesLengthSheet
α-helix1464-147714
α-helix1491-150818
α-helix1518-15247
α-helix1525-15284
α-helix1532-154716
α-helix1553-15608
α-helix1569-15713
α-helix1572-158211
α-helix1590-160314
α-helix1607-16104
α-helix1611-162313
α-helix1625-16273
α-helix1628-16347
α-helix1639-16413
α-helix1643-165715
α-helix1658-16603
α-helix1664-167613
β-strand168716
α-helix1694-17029
α-helix1706-172015
α-helix1727-174115
α-helix1744-175310
α-helix1760-17645
α-helix1765-17673
α-helix1786-17883
α-helix1792-17954
α-helix1802-181514
α-helix1828-18314
α-helix1835-185117
α-helix1857-187418
α-helix1903-191715
α-helix1920-19212
α-helix1927-19304
α-helix1938-194710
α-helix1951-197323
α-helix1986-199611
α-helix2001-201111
α-helix2029-203810
α-helix2042-205110
α-helix2057-207014
α-helix2075-208713
α-helix2093-210513
α-helix2124-213613
α-helix2140-216021
β-strand216516
α-helix2169-218820
α-helix2195-221117
α-helix2217-223620
α-helix2242-226322
α-helix2269-228113
α-helix2291-230111
α-helix2305-232117
α-helix2328-234821
α-helix2353-23564
α-helix2357-23615
α-helix2362-23709
α-helix2377-240529
α-helix2408-242922
α-helix2436-247540
α-helix2481-24833
α-helix2484-249310
α-helix2498-25069
α-helix2508-25103
α-helix2513-25153
α-helix2520-25245
α-helix2535-255117
α-helix2553-25553
α-helix2557-25648
α-helix2568-25725
α-helix2593-261220
α-helix2614-263219
α-helix2636-26383
β-strand2645-264627
α-helix2647-26482
α-helix2652-26554
β-strand266318
β-strand268218
β-strand2683-268649
β-strand2689-269027
β-strand2691-269229
β-strand2700-270679
β-strand2711-271889
α-helix2723-274119
α-helix2743-27486
β-strand2757-275939
β-strand2764-276859
α-helix2769-27702
β-strand2773-2775310
α-helix2776-27805
α-helix2787-27915
α-helix2796-27972
α-helix2798-280811
α-helix2813-282513
α-helix2831-28333
α-helix2834-28385
α-helix2842-286625
α-helix2873-28753
β-strand2876-2879410
β-strand2885-2887310
α-helix2912-29165
α-helix2922-29243
α-helix2926-293914
α-helix2942-29498
α-helix3004-301815
α-helix3028-304013
α-helix3042-30465
α-helix3050-30523

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Cellular tumor antigen p53E, Fprotein12Homo sapiensP04637 (AlphaFold model)
Serine-protein kinase ATMA, Bprotein3184Homo sapiensQ13315
Sequence of entity 1 (E, F), FASTA
>8OXO_1 Cellular tumor antigen p53 (chains E, F)
EPPLSQETFSDL
Sequence of entity 2 (A, B), FASTA
>8OXO_2 Serine-protein kinase ATM (chains A, B)
MDYKDDDDKHMGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFM
LGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLEGG
SAGSGSASMSLVLNDLLICCRQLEHDRATERKKEVEKFKRLIRDPETIKHLDRHSDSKQG
KYLNWDAVFRFLQKYIQKETECLRIAKPNVSASTQASRQKKMQEISSLVKYFIKCANRRA
PRLKCQELLNYIMDTVKDSSNGAIYGADCSNILLKDILSVRKYWCEISQQQWLELFSVYF
RLYLKPSQDVHRVLVARIIHAVTKGCCSQTDGLNSKFLDFFSKAIQCARQEKSSSGLNHI
LAALTIFLKTLAVNFRIRVCELGDEILPTLLYIWTQHRLNDSLKEVIIELFQLQIYIHHP
KGAKTQEKGAYESTKWRSILYNLYDLLVNEISHIGSRGKYSSGFRNIAVKENLIELMADI
CHQVFNEDTRSLEISQSYTTTQRESSDYSVPCKRKKIELGWEVIKDHLQKSQNDFDLVPW
LQIATQLISKYPASLPNCELSPLLMILSQLLPQQRHGERTPYVLRCLTEVALCQDKRSNL
ESSQKSDLLKLWNKIWCITFRGISSEQIQAENFGLLGAIIQGSLVEVDREFWKLFTGSAC
RPSCPAVCCLTLALTTSIVPGTVKMGIEQNMCEVNRSFSLKESIMKWLLFYQLEGDLENS
TEVPPILHSNFPHLVLEKILVSLTMKNCKAAMNFFQSVPECEHHQKDKEELSFSEVEELF
LQTTFDKMDFLTIVRECGIEKHQSSIGFSVHQNLKESLDRCLLGLSEQLLNNYSSEITNS
ETLVRCSRLLVGVLGCYCYMGVIAEEEAYKSELFQKAKSLMQCAGESITLFKNKTNEEFR
IGSLRNMMQLCTRCLSNCTKKSPNKIASGFFLRLLTSKLMNDIADICKSLASFIKKPFDR
GEVESMEDDTNGNLMEVEDQSSMNLFNDYPDSSVSDANEPGESQSTIGAINPLAEEYLSK
QDLLFLDMLKFLCLCVTTAQTNTVSFRAADIRRKLLMLIDSSTLEPTKSLHLHMYLMLLK
ELPGEEYPLPMEDVLELLKPLSNVCSLYRRDQDVCKTILNHVLHVVKNLGQSNMDSENTR
DAQGQFLTVIGAFWHLTKERKYIFSVRMALVNCLKTLLEADPYSKWAILNVMGKDFPVNE
VFTQFLADNHHQVRMLAAESINRLFQDTKGDSSRLLKALPLKLQQTAFENAYLKAQEGMR
EMSHSAENPETLDEIYNRKSVLLTLIAVVLSCSPICEKQALFALCKSVKENGLEPHLVKK
VLEKVSETFGYRRLEDFMASHLDYLVLEWLNLQDTEYNLSSFPFILLNYTNIEDFYRSCY
KVLIPHLVIRSHFDEVKSIANQIQEDWKSLLTDCFPKILVNILPYFAYEGTRDSGMAQQR
ETATKVYDMLKSENLLGKQIDHLFISNLPEIVVELLMTLHEPANSSASQSTDLCDFSGDL
DPAPNPPHFPSHVIKATFAYISNCHKTKLKSILEILSKSPDSYQKILLAICEQAAETNNV
YKKHRILKIYHLFVSLLLKDIKSGLGGAWAFVLRDVIYTLIHYINQRPSCIMDVSLRSFS
LCCDLLSQVCQTAVTYCKDALENHLHVIVGTLIPLVYEQVEVQKQVLDLLKYLVIDNKDN
ENLYITIKLLDPFPDHVVFKDLRITQQKIKYSRGPFSLLEEINHFLSVSVYDALPLTRLE
GLKDLRRQLELHKDQMVDIMRASQDNPQDGIMVKLVVNLLQLSKMAINHTGEKEVLEAVG
SCLGEVGPIDFSTIAIQHSKDASYTKALKLFEDKELQWTFIMLTYLNNTLVEDCVKVRSA
AVTCLKNILATKTGHSFWEIYKMTTDPMLAYLQPFRTSRKKFLEVPRFDKENPFEGLDDI
NLWIPLSENHDIWIKTLTCAFLDSGGTKCEILQLLKPMCEVKTDFCQTVLPYLIHDILLQ
DTNESWRNLLSTHVQGFFTSCLRHFSQTSRSTTPANLDSESEHFFRCCLDKKSQRTMLAV
VDYMRRQKRPSSGTIFNDAFWLDLNYLEVAKVAQSCAAHFTALLYAEIYADKKSMDDQEK
RSLAFEEGSQSTTISSLSEKSKEETGISLQDLLLEIYRSIGEPDSLYGCGGGKMLQPITR
LRTYEHEAMWGKALVTYDLETAIPSSTRQAGIIQALQNLGLCHILSVYLKGLDYENKDWC
PELEELHYQAAWRNMQWDHCTSVSKEVEGTSYHESLYNALQSLRDREFSTFYESLKYARV
KEVEEMCKRSLESVYSLYPTLSRLQAIGELESIGELFSRSVTHRQLSEVYIKWQKHSQLL
KDSDFSFQEPIMALRTVILEILMEKEMDNSQRECIKDILTKHLVELSILARTFKNTQLPE
RAIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKKLDASCAANNPSLKL
TYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSDELRNGKMKAFLSLAR
FSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELAL
RALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIP
TYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFL
TKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILAN
LDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRL
AGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQVFQMCNTLLQRNTETRKRKLTI
CTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKRYRPNDFSAFQCQKKMMEVQKKS
FEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKRLAYTRSVATSSIVGYILGLGDRH
VQNILINEQSAELVHIDLGVAFEQGKILPTPETVPFRLTRDIVDGMGITGVEGVFRRCCE
KTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKALYLAQRPEDETELHPTLNADDQECK
RNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVLSVGGQVNLLIQQAIDPKNLSRLFPGW
KAWV

Ligands and cofactors

IDNameFormulaCopies
ANPPhosphoaminophosphonic acid-adenylate esterC10 H17 N6 O12 P32
MGMagnesium ionMg2
ZNZinc ionZn2

Primary citation

Structural insights into the activation of ataxia-telangiectasia mutated by oxidative stress. Howes, A.C., Perisic, O., Williams, R.L. Sci Adv (2023) 9:eadi8291-eadi8291. DOI 10.1126/sciadv.adi8291 · PubMed

Other PDB entries of the same protein (UniProt P04637 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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