8OZ0: PDB entry 8OZ0
Structure of a human 48S translation initiation complex with eIF4F and eIF4A. Determined by electron microscopy at 3.5 Å resolution. Released 7 Feb 2024.
- Method
- Electron microscopy
- Resolution
- 3.5 Å
- Organism
- Homo sapiens
- Chains
- 57
- Atoms
- 121,610
- Mol. weight
- 2476.48 kDa
- Ligands
- ZN, MG
- Released
- 7 Feb 2024
Explore 8OZ0 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8OZ0 contains 507 α-helices and 471 β-strands across 54 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 1: 17 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 35-37 | 3 | |
| α-helix | 41-49 | 9 | |
| α-helix | 55-56 | 2 | |
| α-helix | 57-67 | 11 | |
| β-strand | 72 | 1 | 24 |
| α-helix | 82-93 | 12 | |
| β-strand | 106 | 1 | 25 |
| α-helix | 110-123 | 14 | |
| α-helix | 141-149 | 9 | |
| β-strand | 157 | 1 | 25 |
| α-helix | 160-167 | 8 | |
| β-strand | 178 | 1 | 26 |
| β-strand | 181 | 1 | 25 |
| α-helix | 184-187 | 4 | |
| α-helix | 191-201 | 11 | |
| β-strand | 208 | 1 | 26 |
| α-helix | 218-223 | 6 | |
| β-strand | 232 | 1 | 24 |
| α-helix | 239-242 | 4 | |
| β-strand | 247-249 | 3 | 27 |
| α-helix | 256-269 | 14 | |
| α-helix | 282-294 | 13 | |
| α-helix | 308-318 | 11 | |
| α-helix | 353-360 | 8 | |
| β-strand | 371-373 | 3 | 27 |
| α-helix | 379-390 | 12 | |
Chain 2: 11 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 755-769 | 15 | |
| α-helix | 776-785 | 10 | |
| α-helix | 791-807 | 17 | |
| α-helix | 814-825 | 12 | |
| α-helix | 839-851 | 13 | |
| α-helix | 854-868 | 15 | |
| α-helix | 874-906 | 33 | |
| α-helix | 913-925 | 13 | |
| α-helix | 929-942 | 14 | |
| α-helix | 952-968 | 17 | |
| α-helix | 973-986 | 14 | |
Chain 3: 19 helices, 13 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 41-50 | 10 | |
| α-helix | 57-68 | 12 | |
| β-strand | 72-75 | 4 | 8 |
| α-helix | 76-77 | 2 | |
| α-helix | 82-93 | 12 | |
| β-strand | 103-106 | 4 | 9 |
| α-helix | 110-123 | 14 | |
| β-strand | 131-134 | 4 | 9 |
| α-helix | 141-149 | 9 | |
| β-strand | 154-157 | 4 | 9 |
| α-helix | 159-168 | 10 | |
| α-helix | 173-175 | 3 | |
| β-strand | 179 | 1 | 9 |
| β-strand | 181 | 1 | 8 |
| α-helix | 190-201 | 12 | |
| β-strand | 210-213 | 4 | 8 |
| α-helix | 218-224 | 7 | |
| α-helix | 225-227 | 3 | |
| α-helix | 231 | 1 | |
| β-strand | 232-235 | 4 | 8 |
| β-strand | 246-247 | 2 | 10 |
| α-helix | 258-269 | 12 | |
| β-strand | 274-277 | 4 | 11 |
| α-helix | 282-294 | 13 | |
| α-helix | 308-318 | 11 | |
| β-strand | 325-327 | 3 | 11 |
| α-helix | 330-333 | 4 | |
| β-strand | 341-345 | 5 | 11 |
| α-helix | 353-362 | 10 | |
| β-strand | 370-371 | 2 | 10 |
| α-helix | 382-391 | 10 | |
| α-helix | 401-404 | 4 | |
Chain 5: 25 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-10 | 4 | |
| α-helix | 16-29 | 14 | |
| α-helix | 34-47 | 14 | |
| α-helix | 52-61 | 10 | |
| α-helix | 64-67 | 4 | |
| α-helix | 68-96 | 29 | |
| α-helix | 98-106 | 9 | |
| α-helix | 110-121 | 12 | |
| α-helix | 125-141 | 17 | |
| α-helix | 144-157 | 14 | |
| α-helix | 164-178 | 15 | |
| α-helix | 183-197 | 15 | |
| α-helix | 204-224 | 21 | |
| α-helix | 228-233 | 6 | |
| α-helix | 234-238 | 5 | |
| α-helix | 240-249 | 10 | |
| α-helix | 252-262 | 11 | |
| α-helix | 267-283 | 17 | |
| α-helix | 291-297 | 7 | |
| α-helix | 298-302 | 5 | |
| α-helix | 305-320 | 16 | |
| α-helix | 330-347 | 18 | |
| β-strand | 352 | 1 | 1 |
| α-helix | 354-359 | 6 | |
| α-helix | 364-377 | 14 | |
| β-strand | 383-385 | 3 | 1 |
| β-strand | 390-392 | 3 | 1 |
| α-helix | 400-433 | 34 | |
Chain 6: 10 helices, 9 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 94 | 1 | 2 |
| α-helix | 98-102 | 5 | |
| α-helix | 104-107 | 4 | |
| β-strand | 116-124 | 9 | 2 |
| β-strand | 129-138 | 10 | 2 |
| β-strand | 140-141 | 2 | 3 |
| β-strand | 146-147 | 2 | 3 |
| α-helix | 150-163 | 14 | |
| β-strand | 175 | 1 | 4 |
| α-helix | 181-193 | 13 | |
| β-strand | 199-203 | 5 | 4 |
| α-helix | 206-208 | 3 | |
| β-strand | 212-218 | 7 | 4 |
| α-helix | 220-222 | 3 | |
| β-strand | 232-235 | 4 | 4 |
| α-helix | 243-255 | 13 | |
| α-helix | 268-294 | 27 | |
| α-helix | 302-313 | 12 | |
| α-helix | 320-356 | 37 | |
Chain 7: 5 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 240-244 | 5 | 5 |
| α-helix | 252-259 | 8 | |
| α-helix | 260-262 | 3 | |
| β-strand | 267-272 | 6 | 5 |
| α-helix | 273 | 1 | |
| β-strand | 279-286 | 8 | 5 |
| α-helix | 290-300 | 11 | |
| β-strand | 302 | 1 | 6 |
| α-helix | 309 | 1 | |
| β-strand | 310 | 1 | 6 |
| β-strand | 311-313 | 3 | 5 |
Chain 8: 12 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 43-48 | 6 | |
| β-strand | 65-72 | 8 | 12 |
| β-strand | 75-83 | 9 | 12 |
| α-helix | 96-108 | 13 | |
| β-strand | 116 | 1 | 12 |
| β-strand | 120 | 1 | 12 |
| β-strand | 122 | 1 | 13 |
| α-helix | 131-143 | 13 | |
| β-strand | 149-151 | 3 | 14 |
| β-strand | 152 | 1 | 13 |
| β-strand | 165-167 | 3 | 14 |
| β-strand | 169 | 1 | 15 |
| α-helix | 171-179 | 9 | |
| α-helix | 184-189 | 6 | |
| β-strand | 198 | 1 | 15 |
| α-helix | 209-220 | 12 | |
| α-helix | 228-231 | 4 | |
| α-helix | 235-269 | 35 | |
| α-helix | 275-291 | 17 | |
| α-helix | 301-305 | 5 | |
| α-helix | 306-310 | 5 | |
| α-helix | 314-350 | 37 | |
Chain 9: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-21 | 19 | |
46 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Eukaryotic translation initiation factor 3 subunit E | 5 | protein | 445 | Homo sapiens | P60228 (AlphaFold model) |
| Eukaryotic translation initiation factor 3 subunit F | 6 | protein | 357 | Homo sapiens | O00303 (AlphaFold model) |
| Eukaryotic translation initiation factor 3 subunit G | 7 | protein | 320 | Homo sapiens | O75821 (AlphaFold model) |
| Eukaryotic translation initiation factor 3 subunit H | 8 | protein | 352 | Homo sapiens | O15372 (AlphaFold model) |
| 60S ribosomal protein L41 | 9 | protein | 25 | Homo sapiens | P62945 |
| Eukaryotic translation initiation factor 3 subunit A | A | protein | 1382 | Homo sapiens | Q14152 |
| Eukaryotic translation initiation factor 3 subunit K | B | protein | 218 | Homo sapiens | Q9UBQ5 |
| Eukaryotic translation initiation factor 3 subunit M | C | protein | 374 | Homo sapiens | Q7L2H7 |
| Eukaryotic translation initiation factor 2 subunit 1 | D | protein | 315 | Homo sapiens | P05198 |
| Eukaryotic translation initiation factor 2 subunit 3 | E | protein | 472 | Homo sapiens | P41091 |
| Eukaryotic translation initiation factor 3 subunit I | F | protein | 325 | Homo sapiens | Q13347 |
| Eukaryotic translation initiation factor 1A, X-chromosomal | G | protein | 144 | Homo sapiens | P47813 |
44 more molecules are not listed.
Sequence of entity 1 (5), FASTA
>8OZ0_1 Eukaryotic translation initiation factor 3 subunit E (chains 5)
MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKN
LYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADK
HGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQ
NWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQP
QYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLY
VNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKL
NMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAM
NIEKKLNQNSRSEAPNWATQDSGFY
Sequence of entity 2 (6), FASTA
>8OZ0_2 Eukaryotic translation initiation factor 3 subunit F (chains 6)
MATPAVPVSAPPATPTPVPAAAPASVPAPTPAPAAAPVPAAAPASSSDPAAAAAATAAPG
QTPASAQAPAQTPAPALPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTL
LGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDIT
EHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYA
YYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSA
DNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL
Sequence of entity 3 (7), FASTA
>8OZ0_3 Eukaryotic translation initiation factor 3 subunit G (chains 7)
MPTGDFDSKPSWADQVEEEGEDDKCVTSELLKGIPLATGDTSPEPELLPGAPLPPPKEVI
NGNIKTVTEYKIDEDGKKFKIVRTFRIETRKASKAVARRKNWKKFGNSEFDPPGPNVATT
TVSDDVSMTFITSKEDLNCQEEEDPMNKLKGQKIVSCRICKGDHWTTRCPYKDTLGPMQK
ELAEQLGLSTGEKEKLPGELEPVQATQNKTGKYVPPSLRDGASRRGESMQPNRRADDNAT
IRVTNLSEDTRETDLQELFRPFGSISRIYLAKDKTTGQSKGFAFISFHRREDAARAIAGV
SGFGYDHLILNVEWAKPSTN
Sequence of entity 4 (8), FASTA
>8OZ0_4 Eukaryotic translation initiation factor 3 subunit H (chains 8)
MASRKEGTGSTATSSSSTAGAAGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGT
EVVQGVLLGLVVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWY
QSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEK
DFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLG
KNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEED
LSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNN
Sequence of entity 5 (9), FASTA
>8OZ0_5 60S ribosomal protein L41 (chains 9)
MRAKWRKKRMRRLKRKRRKMRQRSK
Sequence of entity 6 (A), FASTA
>8OZ0_6 Eukaryotic translation initiation factor 3 subunit A (chains A)
MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVD
LRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDN
IQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQ
AFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSA
ISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLY
HLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLG
LQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEK
EPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDL
QVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPA
HILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAE
LQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIED
LEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIK
DMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFE
ERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEE
LREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWR
KGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRR
GMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRR
GLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPR
RGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPW
RNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEE
REWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWR
DSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDR
VEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTV
RR
Sequence of entity 7 (B), FASTA
>8OZ0_7 Eukaryotic translation initiation factor 3 subunit K (chains B)
MAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAF
FQTTVTAQILLKALTNLPHTDFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQA
LDENMDLLEGITGFEDSVRKFICHVVGITYQHIDRWLLAEMLGDLSDSQLKVWMSKYGWS
ADESGQIFICSQEESIKPKNIVEKIDFDSVSSIMASSQ
Sequence of entity 8 (C), FASTA
>8OZ0_8 Eukaryotic translation initiation factor 3 subunit M (chains C)
MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDV
ESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVR
YTVYCSLIKVAASCGAIQYIPTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDA
ASKVMVELLGSYTEDNASQARVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDL
LTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQE
LQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLYDTLNAWKQN
LNKVKNSLLSLSDT
Sequence of entity 9 (D), FASTA
>8OZ0_9 Eukaryotic translation initiation factor 2 subunit 1 (chains D)
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSIN
KLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLE
YTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNI
NRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTT
TLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEV
DGDDDAEEMEAKAED
Sequence of entity 10 (E), FASTA
>8OZ0_10 Eukaryotic translation initiation factor 2 subunit 3 (chains E)
MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINIGTIGHVAHGKSTVVKA
ISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECYRSCGSSTPDEFPTDIPGT
KGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIM
KLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIV
KKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAGGSILKGVLKVGQEIEVRPGI
VSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRADRMVGQVLGA
VGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAV
KADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD
Sequence of entity 11 (F), FASTA
>8OZ0_11 Eukaryotic translation initiation factor 3 subunit I (chains F)
MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDA
DWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQC
FVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEV
LVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPN
YDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSY
SSGGEDGYVRIHYFDPQYFEFEFEA
Sequence of entity 12 (G), FASTA
>8OZ0_12 Eukaryotic translation initiation factor 1A, X-chromosomal (chains G)
MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH
IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT
FGPGDDDEIQFDDIGDDDEDIDDI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 2 |
| MG | Magnesium ion | Mg | 89 |
Primary citation
The structure of a human translation initiation complex reveals two independent roles for the helicase eIF4A. Brito Querido, J., Sokabe, M., Diaz-Lopez, I. et al. Nat Struct Mol Biol (2024) 31:455-464. DOI 10.1038/s41594-023-01196-0 · PubMed
Other PDB entries of the same protein (UniProt P60228 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 8PPL 2.65 Å, MERS-CoV Nsp1 bound to the human 43S pre-initiation complex
- 28ZX 2.8 Å, human 48S PIC with mRNA (Kozak_1)
- 6ZP4 2.9 Å, SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2
- 8PJ5 2.9 Å, Structure of human 48S translation initiation complex after eIF2 release prior 60S…
- 8PJ6 2.9 Å, Structure of human 48S translation initiation complex with initiator tRNA, eIF1A and…
- 28ZY 3.0 Å, human 48S PIC with mRNA (Kozak_2)
- 6ZON 3.0 Å, SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 1
- 9KZU 3.0 Å, Cryo-EM structure of the HCV IRES-dependently initiated CMV-stalled 80S ribosome…
- 8PJ4 3.2 Å, Structure of human 48S translation initiation complex after eIF5 release (48S-4)
- 9KN5 3.2 Å, Structure of the human 40S ribosome complexed with HCV IRES, eIF1A and eIF3
- 9KRP 3.2 Å, Structure of the HCV IRES-dependent 48S translation initiation complex with eIF5B and eIF3
- 6YBD 3.3 Å, Structure of a human 48S translational initiation complex - eIF3
Browse structure collections
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