8QYQ: Myosin-7

Beta-cardiac myosin S1 fragment in the pre-powerstroke state complexed to Mavacamten. Determined by X-ray diffraction at 2.61 Å resolution. Released 13 Dec 2023.

Method: X-ray diffraction
Resolution: 2.61 Å
Organism: Bos taurus
Chains: 4
Atoms: 14,993
Mol. weight: 230.51 kDa
Ligands: XB2, MG, BEF, ADP
Released: 13 Dec 2023

Explore 8QYQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8QYQ contains 91 α-helices and 60 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 38 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	4-15	12
α-helix	20-26	7
β-strand	36-41	6	1
β-strand	45-54	10	1
β-strand	59-63	5	1
β-strand	68-71	4	1
β-strand	77-78	2	1
α-helix	79-81	3
α-helix	82-84	3
β-strand	89	1	2
α-helix	90-92	3
α-helix	98-109	12
β-strand	115-118	4	2
β-strand	121-125	5	2
α-helix	136-142	7
α-helix	154-168	15
β-strand	172-177	6	2
α-helix	184-201	18
α-helix	216-231	16
β-strand	232-233	2	3
β-strand	241-242	2	3
β-strand	245-252	8	2
β-strand	258-266	9	2
α-helix	270-273	4
β-strand	283	1	3
α-helix	284-290	7
α-helix	295-301	7
α-helix	307-309	3
α-helix	325-338	14
α-helix	343-359	17
β-strand	364-366	3	4
β-strand	373-375	3	4
α-helix	379-388	10
α-helix	392-400	9
β-strand	403-405	3	5
β-strand	410-412	3	5
α-helix	413-414	2
α-helix	417-447	31
β-strand	455-461	7	2
α-helix	462-465	4
β-strand	471	1	6
α-helix	473-490	18
α-helix	491-496	6
α-helix	497-503	7
α-helix	518-525	8
α-helix	530-537	8
α-helix	545-556	12
β-strand	563-564	2	6
β-strand	577-581	5	6
β-strand	584-588	5	6
α-helix	593-598	6
α-helix	603-610	8
α-helix	615-621	7
α-helix	647-663	17
β-strand	666-673	8	2
α-helix	686-696	11
α-helix	698-707	10
β-strand	711-714	4	7
α-helix	715-722	8
α-helix	723-725	3
α-helix	727-729	3
α-helix	738-748	11
β-strand	756-758	3	7
β-strand	762-765	4	7
α-helix	769-806	38

Chain B: 39 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	7-15	9
α-helix	20-26	7
β-strand	36-40	5	8
β-strand	46-54	9	8
β-strand	58-63	6	8
β-strand	68-72	5	8
α-helix	73-75	3
β-strand	77-78	2	8
α-helix	79-81	3
α-helix	82-84	3
β-strand	89	1	9
α-helix	90-92	3
α-helix	98-109	12
β-strand	115-118	4	9
β-strand	121-125	5	9
α-helix	136-142	7
α-helix	154-168	15
β-strand	172-177	6	9
α-helix	184-201	18
α-helix	216-231	16
β-strand	232-233	2	10
β-strand	241-242	2	10
β-strand	245-252	8	9
β-strand	258-266	9	9
α-helix	270-273	4
β-strand	283	1	10
α-helix	284-290	7
α-helix	295-301	7
α-helix	307-309	3
α-helix	325-338	14
α-helix	343-359	17
β-strand	364-366	3	11
β-strand	373-375	3	11
α-helix	379-388	10
α-helix	392-400	9
β-strand	403-406	4	12
β-strand	409-412	4	12
α-helix	413-414	2
α-helix	417-447	31
β-strand	455-461	7	9
α-helix	462-465	4
β-strand	471	1	13
α-helix	473-490	18
α-helix	491-496	6
α-helix	497-503	7
α-helix	518-525	8
α-helix	530-537	8
α-helix	545-556	12
β-strand	563-564	2	13
β-strand	577-581	5	13
β-strand	584-588	5	13
α-helix	593-598	6
α-helix	603-610	8
α-helix	615-621	7
α-helix	647-663	17
β-strand	666-673	8	9
α-helix	686-696	11
α-helix	698-707	10
β-strand	711-714	4	14
α-helix	715-722	8
α-helix	723-725	3
α-helix	727-729	3
α-helix	738-748	11
β-strand	756-758	3	14
β-strand	762-765	4	14
α-helix	769-797	29

Chain C: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	52-64	13
β-strand	74-76	3	15
α-helix	80-86	7
α-helix	93-99	7
α-helix	105-108	4
β-strand	112-114	3	15
α-helix	115-125	11
α-helix	134-142	9
β-strand	151-153	3	16
α-helix	154-163	10
α-helix	170-176	7
β-strand	186-188	3	16
α-helix	189-197	9

Chain D: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	81-86	6
α-helix	93-99	7
α-helix	134-142	9
β-strand	151-153	3	17
α-helix	154-163	10
β-strand	186-188	3	17
α-helix	189-197	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Myosin-7	A, B	protein	807	Bos taurus	Q9BE39 (AlphaFold model)
Myosin light chain 3	C	protein	199	Bos taurus	P85100 (AlphaFold model)
Myosin light chain 3	D	protein	199	Bos taurus	P85100 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>8QYQ_1 Myosin-7 (chains A, B)
MVDAEMAAFGEAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKATILSREGGKVT
AETEHGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGL
FCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGES
GAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDN
SSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDM
LLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTTEEKNSMYKLTGAIMHFG
NMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQV
VYAKGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINF
TNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMF
PKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNIIGWLQKNKDP
LNETVVDLYKKSSLKMLSSLFANYAGFDTPIEKGKGKAKKGSSFQTVSALHRENLNKLMT
NLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQ
RYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDER
LSRIITRIQAQSRGVLSRMEFKKLLER

Sequence of entity 2 (C), FASTA

>8QYQ_2 Myosin light chain 3 (chains C)
MAPKKPDPKKDEAKAGAKAAAAPAPAPAPPPAPEPSKEPEFDPSKIKIEFTPEQIEEFKE
AFTLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPKQEELNSKMMDFDTFLP
MLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKLTEDEVEKLMAGQ
EDSNGCINYEAFVKHIMAG

Sequence of entity 3 (D), FASTA

>8QYQ_3 Myosin light chain 3 (chains D)
MAPKKPDPKKDEAKAGAKAAAAPAPAPAPPPAPEPSKEPEFDPSKIKIEFTPEQIEEFKE
AFTLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPKQEELNSKMMDFDTFLP
MLQHISKNKDTGTYEDFVEGLRVFDKEGNGVVMGAELRHVLATLGEKLTEDEVEKLMAGQ
EDSNGCINYEAFVKHIMAG

Ligands and cofactors

ID	Name	Formula	Copies
XB2	Mavacamten	C15 H19 N3 O2	2
MG	Magnesium ion	Mg	2
BEF	Beryllium trifluoride ion	Be F3	2
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	2

Water and common crystallization additives (GOL, FMT) are not listed.

Primary citation

Omecamtiv mecarbil and Mavacamten target the same myosin pocket despite antagonistic effects in heart contraction. Auguin, D., Robert-Paganin, J., Rety, S. et al. bioRxiv (2023). DOI 10.1101/2023.11.15.567213 · PubMed

Other PDB entries of the same protein (UniProt Q9BE39 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QYR 1.8 Å, Beta-cardiac myosin motor domain in the pre-powerstroke state complexed to Mavacamten
8QYU 1.96 Å, Beta-cardiac myosin S1 fragment in the pre-powerstroke state complexed to Omecamtiv…
6FSA 2.33 Å, Beta-Cardiac myosin post-rigor
9F6C 2.33 Å, Cardiac myosin motor domain in the pre-powerstroke state co-crystallized with the…
5N69 2.45 Å, Cardiac muscle myosin S1 fragment in the pre-powerstroke state co-crystallized with the…
8QYP 2.76 Å, Beta-cardiac myosin motor domain in the pre-powerstroke state
5N6A 3.1 Å, Cardiac muscle myosin motor domain in the pre-powerstroke state
6X5Z 4.24 Å, Bovine Cardiac Myosin in Complex with Chicken Skeletal Actin and Human Cardiac…

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