8R7L: Human TREX complex

Cryo-EM structure of the human TREX complex. Determined by electron microscopy at 4.12 Å resolution. Released 11 Jun 2025.

Method: Electron microscopy
Resolution: 4.12 Å
Organism: Homo sapiens
Chains: 5
Atoms: 14,961
Mol. weight: 365.95 kDa
Released: 11 Jun 2025

Explore 8R7L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8R7L contains 92 α-helices and 46 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	45-64	20
α-helix	72-83	12
α-helix	92-103	12
α-helix	107-119	13
α-helix	134-146	13
α-helix	153-166	14
α-helix	232-246	15
α-helix	248-250	3
α-helix	255-274	20
α-helix	310-313	4
α-helix	315-333	19
α-helix	347-365	19
α-helix	371-388	18
α-helix	436-442	7
α-helix	490-502	13
α-helix	518-527	10

Chain B: 57 helices, 0 β-strands

Element	Residues	Length
α-helix	143-155	13
α-helix	169-179	11
α-helix	189-203	15
α-helix	207-220	14
α-helix	226-236	11
α-helix	243-253	11
α-helix	264-275	12
α-helix	281-286	6
α-helix	293-307	15
α-helix	344-352	9
α-helix	359-365	7
α-helix	373-375	3
α-helix	377-398	22
α-helix	401	1
α-helix	426	1
α-helix	427-431	5
α-helix	432-436	5
α-helix	442-444	3
α-helix	446-463	18
α-helix	474-486	13
α-helix	487-491	5
α-helix	492-495	4
α-helix	504-512	9
α-helix	517-529	13
α-helix	536-553	18
α-helix	565-574	10
α-helix	576-590	15
α-helix	594-600	7
α-helix	606-621	16
α-helix	637-652	16
α-helix	659-670	12
α-helix	676-685	10
α-helix	697-701	5
α-helix	708-712	5
α-helix	723-736	14
α-helix	739-755	17
α-helix	765-787	23
α-helix	790-796	7
α-helix	800-806	7
α-helix	811-822	12
α-helix	861-868	8
α-helix	878-886	9
α-helix	900-907	8
α-helix	929-960	32
α-helix	973-977	5
α-helix	978-982	5
α-helix	983-988	6
α-helix	990-998	9
α-helix	999-1003	5
α-helix	1004-1005	2
α-helix	1013-1022	10
α-helix	1024-1029	6
α-helix	1035-1054	20
α-helix	1091-1112	22
α-helix	1118-1129	12
α-helix	1138-1153	16
α-helix	1161-1175	15

Chain C: 1 helix, 30 β-strands

Element	Residues	Length	Sheet
α-helix	33-44	12
β-strand	47	1	4
β-strand	50-54	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-84	7	6
β-strand	89-96	8	6
β-strand	104-107	4	7
β-strand	114-118	5	7
β-strand	123-128	6	7
β-strand	133-139	7	7
β-strand	144-149	6	8
β-strand	155-160	6	8
β-strand	164-169	6	8
β-strand	175-180	6	8
β-strand	185-190	6	9
β-strand	196-201	6	9
β-strand	205-210	6	9
β-strand	215-221	7	9
β-strand	227-232	6	10
β-strand	238-243	6	10
β-strand	248-252	5	10
β-strand	258-262	5	10
β-strand	269-274	6	11
β-strand	280-285	6	11
β-strand	289-294	6	11
β-strand	303-305	3	11
β-strand	313-315	3	5
β-strand	322-324	3	5
β-strand	340-345	6	5
β-strand	346	1	4

Chain D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	4-6	3
α-helix	9-20	12

Chain H: 16 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	54-63	10
α-helix	70-79	10
β-strand	85-88	4	1
α-helix	96-106	11
β-strand	116-119	4	1
α-helix	123-136	14
β-strand	145-148	4	1
α-helix	154-162	9
β-strand	168-171	4	1
α-helix	173-180	8
β-strand	192-196	5	1
α-helix	198-203	6
α-helix	205-217	13
β-strand	223-228	6	1
α-helix	236-239	4
α-helix	240-242	3
β-strand	247-249	3	1
β-strand	258	1	2
β-strand	262-268	7	3
α-helix	274-284	11
β-strand	289-293	5	3
α-helix	297-309	13
β-strand	314-317	4	3
α-helix	323-334	12
β-strand	340-343	4	3
β-strand	356-361	6	3
α-helix	368-375	8
β-strand	377	1	2
β-strand	385-391	7	3
α-helix	394-407	14
β-strand	411-413	3	3
α-helix	414	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Spliceosome RNA helicase DDX39B	H	protein	428	Homo sapiens	Q13838 (AlphaFold model)
THO complex subunit 1	A	protein	657	Homo sapiens	Q96FV9 (AlphaFold model)
THO complex subunit 2	B	protein	1593	Homo sapiens	Q8NI27 (AlphaFold model)
THO complex subunit 3	C	protein	351	Homo sapiens	Q96J01 (AlphaFold model)
THO complex subunit 4	D	protein	183	Homo sapiens	Q86V81

Sequence of entity 1 (H), FASTA

>8R7L_1 Spliceosome RNA helicase DDX39B (chains H)
MAENDVDNELLDYEDDEVETAAGGDGAEAPAKKDVKGSYVSIHSSGFRDFLLKPELLRAI
VDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTAVFVLATLQQLEPVTGQVSVLVMC
HTRELAFQISKEYERFSKYMPNVKVAVFFGGLSIKKDEEVLKKNCPHIVVGTPGRILALA
RNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRMTPHEKQVMMFSATLSKEIRPVCR
KFMQDPMEIFVDDETKLTLHGLQQYYVKLKDNEKNRKLFDLLDVLEFNQVVIFVKSVQRC
IALAQLLVEQNFPAIAIHRGMPQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIAF
NYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNISELPDEIDI
SSYIEQTR

Sequence of entity 2 (A), FASTA

>8R7L_2 THO complex subunit 1 (chains A)
MSPTPPLFSLPEARTRFTKSTREALNNKNIKPLLSTFSQVPGSENEKKCTLDQAFRGILE
EEIINHSSCENVLAIISLAIGGVTEGICTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNV
ATWKSNTFYSAGKNYLLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQ
FNLENVTVFNTNEQESTLGQKHTEDREEGMDVEEGEMGDEEAPTTCSIPIDYNLYRKFWS
LQDYFRNPVQCYEKISWKTFLKYSEEVLAVFKSYKLDDTQASRKKMEELKTGGEHVYFAK
FLTSEKLMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQSLWIEDTTKSV
YQLLSENPPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERTSDTKPTRIIRKRTAPE
DFLGKGPTKKILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMV
ENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEI
KTGEDEDEEDNDALLKENESPDVRRDKPVTGEQIEVFANKLGEQWKILAPYLEMKDSEIR
QIECDSEDMKMRAKQLLVAWQDQEGVHATPENLINALNKSGLSDLAESLTNDNETNS

Sequence of entity 3 (B), FASTA

>8R7L_3 THO complex subunit 2 (chains B)
MAAAAVVVPAEWIKNWEKSGRGEFLHLCRILSENKSHDSSTYRDFQQALYELSYHVIKGN
LKHEQASNVLSDISEFREDMPSILADVFCILDIETNCLEEKSKRDYFTQLVLACLYLVSD
TVLKERLDPETLESLGLIKQSQQFNQKSVKIKTKLFYKQQKFNLLREENEGYAKLIAELG
QDLSGSITSDLILENIKSLIGCFNLDPNRVLDVILEVFECRPEHDDFFISLLESYMSMCE
PQTLCHILGFKFKFYQEPNGETPSSLYRVAAVLLQFNLIDLDDLYVHLLPADNCIMDEHK
REIAEAKQIVRKLTMVVLSSEKMDEREKEKEKEEEKVEKPPDNQKLGLLEALLKIGDWQH
AQNIMDQMPPYYAASHKLIALAICKLIHITIEPLYRRVGVPKGAKGSPVNALQNKRAPKQ
AESFEDLRRDVFNMFCYLGPHLSHDPILFAKVVRIGKSFMKEFQSDGSKQEDKEKTEVIL
SCLLSITDQVLLPSLSLMDCNACMSEELWGMFKTFPYQHRYRLYGQWKNETYNSHPLLVK
VKAQTIDRAKYIMKRLTKENVKPSGRQIGKLSHSNPTILFDYILSQIQKYDNLITPVVDS
LKYLTSLNYDVLAYCIIEALANPEKERMKHDDTTISSWLQSLASFCGAVFRKYPIDLAGL
LQYVANQLKAGKSFDLLILKEVVQKMAGIEITEEMTMEQLEAMTGGEQLKAEGGYFGQIR
NTKKSSQRLKDALLDHDLALPLCLLMAQQRNGVIFQEGGEKHLKLVGKLYDQCHDTLVQF
GGFLASNLSTEDYIKRVPSIDVLCNEFHTPHDAAFFLSRPMYAHHISSKYDELKKSEKGS
KQQHKVHKYITSCEMVMAPVHEAVVSLHVSKVWDDISPQFYATFWSLTMYDLAVPHTSYE
REVNKLKVQMKAIDDNQEMPPNKKKKEKERCTALQDKLLEEEKKQMEHVQRVLQRLKLEK
DNWLLAKSTKNETITKFLQLCIFPRCIFSAIDAVYCARFVELVHQQKTPNFSTLLCYDRV
FSDIIYTVASCTENEASRYGRFLCCMLETVTRWHSDRATYEKECGNYPGFLTILRATGFD
GGNKADQLDYENFRHVVHKWHYKLTKASVHCLETGEYTHIRNILIVLTKILPWYPKVLNL
GQALERRVHKICQEEKEKRPDLYALAMGYSGQLKSRKSYMIPENEFHHKDPPPRNAVASV
QNGPGGGPSSSSIGSASKSDESSTEETDKSRERSQCGVKAVNKASSTTPKGNSSNGNSGS
NSNKAVKENDKEKGKEKEKEKKEKTPATTPEARVLGKDGKEKPKEERPNKDEKARETKER
TPKSDKEKEKFKKEEKAKDEKFKTTVPNAESKSTQEREREKEPSRERDIAKEMKSKENVK
GGEKTPVSGSLKSPVPRSDIPEPEREQKRRKIDTHPSPSHSSTVKDSLIELKESSAKLYI
NHTPPPLSKSKEREMDKKDLDKSRERSREREKKDEKDRKERKRDHSNNDREVPPDLTKRR
KEENGTMGVSKHKSESPCESPYPNEKDKEKNKSKSSGKEKGSDSFKSEKMDKISSGGKKE
SRHDKEKIEKKEKRDSSGGKEEKKHHKSSDKHR

Sequence of entity 4 (C), FASTA

>8R7L_4 THO complex subunit 3 (chains C)
MAVPAAAMGPSALGQSGPGSMAPWCSVSSGPSRYVLGMQELFRGHSKTREFLAHSAKVHS
VAWSCDGRRLASGSFDKTASVFLLEKDRLVKENNYRGHGDSVDQLCWHPSNPDLFVTASG
DKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRSKAEEQ
FKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPSNCICIKFDPMGKYFA
TGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASASEDHFIDIAEVETGDK
LWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLFGLPNDS

Sequence of entity 5 (D), FASTA

>8R7L_5 THO complex subunit 4 (chains D)
MADKMDMSLDDIIKLNRSQRGGRGGGRGRGRAGSQGGRGGGAQAAARVNRGGGPIRNRPA
IARGAAGGGGRNRPAPYSRPKQLPDKWQHDLFDSGFGGGAGVETGGKLLVSNLDFGVSDA
DIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQL
VTS

Primary citation

An ATP-gated molecular switch orchestrates human messenger RNA export. Hohmann, U., Graf, M., Tirian, L. et al. Nature (2025). DOI 10.1038/s41586-025-09832-z · PubMed

Other PDB entries of the same protein (UniProt Q13838 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1T5I 1.9 Å, Crystal structure of the C-terminal domain of UAP56
1T6N 1.94 Å, Crystal structure of the N-terminal domain of human UAP56
1XTI 1.95 Å, Structure of Wildtype human UAP56
1XTK 2.4 Å, structure of DECD to DEAD mutation of human UAP56
8ENK 2.5 Å, Crystal structure of UAP56 in complex with Tho1, the yeast homolog of human SARNP
9RV1 2.62 Å, Cryo-EM structure of the human UAP56-RNA - SAC3D1-PCID2-SEM1 complex
1XTJ 2.7 Å, structure of human UAP56 in complex with ADP
9DLP 2.79 Å, Cryo-EM structure of human TREX-2 complex bound to DDX39B(UAP56)
9T6L 2.9 Å, Cryo-EM structure of the human LENG8-PCID2-DSS1 complex bound to UAP56 and RRP1B
9DLV 2.97 Å, Cryo-EM structure of the human TREX-2.1 complex (LENG8/PCID2/DSS1) bound to DDX39B(UAP56)
9T6N 3.0 Å, Cryo-EM structure of the human GANP-PCID2-DSS1 complex bound to UAP56
9DLR 3.08 Å, Cryo-EM structure of the human TREX-2.1 complex (LENG8/PCID2/DSS1) bound to the…

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