8RCN: Serine/threonine-protein kinase mTOR

CryoEM structure of mTORC1 with a paediatric kidney cancer-associated 1455-EWED-1458 duplication in mTOR, Focused region of mTOR and RAPTOR on one protomer copy. Determined by electron microscopy at 3.1 Å resolution. Released 11 Sept 2024.

Method
Electron microscopy
Resolution
3.1 Å
Organism
Homo sapiens
Chains
3
Atoms
14,999
Mol. weight
451.61 kDa
Ligands
ANP, MG
Released
11 Sept 2024

Explore 8RCN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8RCN contains 118 α-helices and 22 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 104 helices, 12 β-strands

ElementResiduesLengthSheet
α-helix776-7849
α-helix792-80918
α-helix814-82916
α-helix831-8333
α-helix837-85014
α-helix857-8604
α-helix864-87411
α-helix878-89114
α-helix896-9027
α-helix934-9374
α-helix938-9403
α-helix943-96018
α-helix964-98219
α-helix983-9897
α-helix990-100314
α-helix1006-102318
α-helix1028-10303
α-helix1033-10419
α-helix1046-106217
α-helix1065-10695
α-helix1071-10733
α-helix1075-10817
α-helix1088-10903
α-helix1091-110111
α-helix1104-11074
α-helix1111-112313
α-helix1129-114214
α-helix1149-11513
α-helix1152-116514
α-helix1167-118418
α-helix1185-11906
α-helix1192-120110
α-helix1207-121711
α-helix1263-12664
α-helix1268-12736
α-helix1277-129317
α-helix1298-13014
α-helix1304-13074
α-helix1311-13166
α-helix1318-132710
α-helix1331-134717
α-helix1351-136515
α-helix1377-13793
α-helix1380-138910
α-helix1393-140412
α-helix1410-142213
α-helix1426-143914
α-helix1514-15229
α-helix1529-15357
α-helix1541-15477
α-helix1551-15544
α-helix1557-157822
α-helix1582-160726
α-helix1611-162515
α-helix1630-164011
α-helix1646-16483
α-helix1650-165910
α-helix1662-167716
α-helix1688-16903
α-helix1694-170512
α-helix1710-173021
α-helix1737-176226
α-helix1769-178214
α-helix1787-180721
α-helix1808-18125
α-helix1868-189326
α-helix1900-191314
α-helix1917-192913
α-helix1932-19387
α-helix1939-19446
α-helix1951-196515
α-helix1969-19713
α-helix1975-19784
α-helix1979-19813
α-helix1985-200117
α-helix2003-202018
α-helix2023-203513
α-helix2036-20416
α-helix2044-20496
α-helix2052-20576
α-helix2065-209127
α-helix2096-211217
β-strand2120-212231
α-helix2123-21253
α-helix2128-21314
β-strand213912
α-helix21401
α-helix2149-21513
β-strand215212
β-strand2153-215641
β-strand2159-216241
α-helix2163-21642
β-strand2170-217671
β-strand2181-218881
α-helix2193-221119
α-helix2213-22186
β-strand2227-223151
β-strand2234-223851
α-helix2239-22402
β-strand2243-224533
α-helix2246-225611
α-helix2263-22719
α-helix2275-22773
α-helix2280-229213
α-helix2298-23058
α-helix2310-233425
β-strand2344-234743
β-strand2353-235533
α-helix2363-23664
α-helix2381-23844
α-helix2395-240915
α-helix2411-242212
α-helix2425-243511
α-helix2493-250917
α-helix2521-253212
α-helix2535-25395
Chain Y: 14 helices, 10 β-strands
ElementResiduesLengthSheet
β-strand57-6374
β-strand8515
β-strand8815
α-helix90-923
α-helix95-11319
β-strand119-12244
α-helix127-13913
β-strand145-15064
α-helix156-1583
β-strand16416
β-strand17516
α-helix179-1857
β-strand190-19454
α-helix199-21719
β-strand241-24444
α-helix262-2687
α-helix270-28112
α-helix292-2954
α-helix308-32720
α-helix330-3378
α-helix341-35717
α-helix358-3603
β-strand365-36624
α-helix378-39114

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Serine/threonine-protein kinase mTORBprotein2549Homo sapiensP42345 (AlphaFold model)
Regulatory-associated protein of mTORYprotein1335Homo sapiensQ8N122 (AlphaFold model)
Eukaryotic translation initiation factor 4E-binding protein 1Xprotein118Homo sapiensQ13541 (AlphaFold model)
Sequence of entity 1 (B), FASTA
>8RCN_1 Serine/threonine-protein kinase mTOR (chains B)
MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEES
TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP
VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP
TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE
AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESR
CCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV
LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA
TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL
KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT
QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL
VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS
MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI
LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA
LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK
VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR
DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV
SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR
VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD
RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV
RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV
STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGI
VLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEK
WTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLA
QQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
ERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVD
PSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHK
QELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEA
VLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSP
TPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVN
EALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVAS
KSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFG
ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA
WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSL
QVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNL
SIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTL
MQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRH
PSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITC
HTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG
VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLD
VPTQVELLIKQATSHENLCQCYIGWCPFW
Sequence of entity 2 (Y), FASTA
>8RCN_2 Regulatory-associated protein of mTOR (chains Y)
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSV
ALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYK
QSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIY
DLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNC
IQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPG
RLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSY
NCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL
TMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLL
QSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILA
VIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSA
HEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVR
KELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSV
SSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILS
FETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYK
ATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRD
LPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFC
DWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLN
RNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQD
CSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMS
SGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALS
ECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALD
IHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGS
NDYYISVYSVEKRVR
Sequence of entity 3 (X), FASTA
>8RCN_3 Eukaryotic translation initiation factor 4E-binding protein 1 (chains X)
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLM
ECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI

Ligands and cofactors

IDNameFormulaCopies
ANPPhosphoaminophosphonic acid-adenylate esterC10 H17 N6 O12 P31
MGMagnesium ionMg2

Primary citation

CryoEM structure of mTORC1 with a paediatric kidney cancer-associated 1455-EWED-1458 duplication in mTOR, Focused region of mTOR and RAPTOR on one protomer copy. Anandapadamanaban, M., Hay, I.M., Perisic, O. et al. To be published.

Other PDB entries of the same protein (UniProt P42345 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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