8RO2: PDB entry 8RO2
Integrative Structure of the human intron lariat Spliceosome (ILS''). Determined by electron microscopy at 3.5 Å resolution. Released 18 Sept 2024.
- Method
- Electron microscopy
- Resolution
- 3.5 Å
- Organism
- Homo sapiens
- Chains
- 40
- Atoms
- 81,753
- Mol. weight
- 2326.67 kDa
- Ligands
- GTP, ZN, IHP
- Released
- 18 Sept 2024
Explore 8RO2 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8RO2 contains 626 α-helices and 386 β-strands across 36 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 3: 4 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 37-38 | 2 | 107 |
| α-helix | 40-55 | 16 | |
| α-helix | 59-63 | 5 | |
| α-helix | 148-154 | 7 | |
| β-strand | 156-157 | 2 | 107 |
| α-helix | 158-176 | 19 | |
Chain a: 5 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-13 | 9 | |
| β-strand | 17-22 | 6 | 71 |
| β-strand | 27-35 | 9 | 71 |
| α-helix | 40 | 1 | |
| β-strand | 41-49 | 9 | 71 |
| α-helix | 50 | 1 | |
| β-strand | 55-63 | 9 | 71 |
| α-helix | 65-67 | 3 | |
| β-strand | 68-72 | 5 | 71 |
| α-helix | 75-79 | 5 | |
Chain A: 110 helices, 49 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-44 | 18 | |
| α-helix | 47-49 | 3 | |
| α-helix | 55-57 | 3 | |
| α-helix | 59-62 | 4 | |
| α-helix | 63-73 | 11 | |
| α-helix | 82-84 | 3 | |
| α-helix | 85-90 | 6 | |
| α-helix | 91-94 | 4 | |
| α-helix | 95-104 | 10 | |
| α-helix | 106-108 | 3 | |
| β-strand | 115-121 | 7 | 1 |
| β-strand | 127-129 | 3 | 1 |
| β-strand | 134-135 | 2 | 2 |
| α-helix | 137-157 | 21 | |
| α-helix | 163-165 | 3 | |
| α-helix | 174-177 | 4 | |
| α-helix | 178-181 | 4 | |
| α-helix | 186-189 | 4 | |
| α-helix | 201-204 | 4 | |
| β-strand | 220 | 1 | 3 |
| β-strand | 227-229 | 3 | 3 |
| α-helix | 232-241 | 10 | |
| α-helix | 242-244 | 3 | |
| α-helix | 252-255 | 4 | |
| α-helix | 260-268 | 9 | |
| α-helix | 299-301 | 3 | |
| α-helix | 311-315 | 5 | |
| α-helix | 329-331 | 3 | |
| α-helix | 357-358 | 2 | |
| α-helix | 371-373 | 3 | |
| α-helix | 396-403 | 8 | |
| α-helix | 404-406 | 3 | |
| β-strand | 415-417 | 3 | 3 |
| β-strand | 419-420 | 2 | 2 |
| α-helix | 428-431 | 4 | |
| α-helix | 441-460 | 20 | |
| α-helix | 467-469 | 3 | |
| α-helix | 472-477 | 6 | |
| β-strand | 482-488 | 7 | 1 |
| α-helix | 489-510 | 22 | |
| β-strand | 516-518 | 3 | 4 |
| β-strand | 524-526 | 3 | 4 |
| α-helix | 529-531 | 3 | |
| α-helix | 532-537 | 6 | |
| α-helix | 542-565 | 24 | |
| α-helix | 571-583 | 13 | |
| α-helix | 585-589 | 5 | |
| α-helix | 591-593 | 3 | |
| α-helix | 596-598 | 3 | |
| α-helix | 599-616 | 18 | |
| α-helix | 631-661 | 31 | |
| α-helix | 677-696 | 20 | |
| α-helix | 706-721 | 16 | |
| α-helix | 733-763 | 31 | |
| α-helix | 769-797 | 29 | |
| α-helix | 805-819 | 15 | |
| α-helix | 822-824 | 3 | |
| β-strand | 825 | 1 | 5 |
| α-helix | 826-829 | 4 | |
| α-helix | 835-847 | 13 | |
| α-helix | 848-850 | 3 | |
| α-helix | 858-872 | 15 | |
| α-helix | 874-887 | 14 | |
| β-strand | 894-900 | 7 | 6 |
| β-strand | 905-910 | 6 | 6 |
| α-helix | 913-933 | 21 | |
| β-strand | 940 | 1 | 7 |
| β-strand | 943 | 1 | 8 |
| β-strand | 946 | 1 | 8 |
| α-helix | 948-960 | 13 | |
| β-strand | 973-980 | 8 | 7 |
| α-helix | 984-987 | 4 | |
| α-helix | 990-1000 | 11 | |
| β-strand | 1001 | 1 | 5 |
| α-helix | 1003-1011 | 9 | |
| β-strand | 1016-1019 | 4 | 6 |
| β-strand | 1022-1025 | 4 | 6 |
| α-helix | 1037-1053 | 17 | |
| α-helix | 1055-1062 | 8 | |
| α-helix | 1077-1080 | 4 | |
| β-strand | 1085-1090 | 6 | 7 |
| β-strand | 1094-1101 | 8 | 7 |
| α-helix | 1103-1116 | 14 | |
| α-helix | 1140-1141 | 2 | |
| α-helix | 1144-1160 | 17 | |
| β-strand | 1174-1178 | 5 | 7 |
| β-strand | 1185-1187 | 3 | 9 |
| β-strand | 1192-1198 | 7 | 9 |
| α-helix | 1199-1203 | 5 | |
| β-strand | 1213-1217 | 5 | 9 |
| β-strand | 1224-1232 | 9 | 9 |
| α-helix | 1234-1247 | 14 | |
| α-helix | 1252-1254 | 3 | |
| α-helix | 1256-1274 | 19 | |
| α-helix | 1275-1277 | 3 | |
| α-helix | 1282-1301 | 20 | |
| α-helix | 1314-1317 | 4 | |
| α-helix | 1320-1322 | 3 | |
| α-helix | 1337-1340 | 4 | |
| β-strand | 1341-1346 | 6 | 10 |
| β-strand | 1349-1354 | 6 | 10 |
| α-helix | 1369-1371 | 3 | |
| α-helix | 1375-1398 | 24 | |
| α-helix | 1405-1407 | 3 | |
| α-helix | 1409-1411 | 3 | |
| α-helix | 1419-1423 | 5 | |
| α-helix | 1427-1430 | 4 | |
| α-helix | 1436-1442 | 7 | |
| α-helix | 1443-1445 | 3 | |
| α-helix | 1458-1461 | 4 | |
| α-helix | 1468-1477 | 10 | |
| α-helix | 1481-1484 | 4 | |
| α-helix | 1485-1487 | 3 | |
| α-helix | 1490-1493 | 4 | |
| β-strand | 1501-1502 | 2 | 11 |
| α-helix | 1512-1516 | 5 | |
| α-helix | 1520-1522 | 3 | |
| α-helix | 1529-1531 | 3 | |
| α-helix | 1532-1537 | 6 | |
| α-helix | 1539-1542 | 4 | |
| β-strand | 1552-1554 | 3 | 12 |
| β-strand | 1561-1563 | 3 | 12 |
| α-helix | 1569-1576 | 8 | |
| α-helix | 1581-1597 | 17 | |
| β-strand | 1606-1611 | 6 | 13 |
| α-helix | 1616-1620 | 5 | |
| β-strand | 1629-1636 | 8 | 13 |
| β-strand | 1643-1644 | 2 | 13 |
| β-strand | 1657-1667 | 11 | 13 |
| α-helix | 1677-1688 | 12 | |
| β-strand | 1700-1706 | 7 | 13 |
| β-strand | 1711-1716 | 6 | 13 |
| α-helix | 1722-1736 | 15 | |
| α-helix | 1738-1751 | 14 | |
| β-strand | 1754-1755 | 2 | 11 |
| α-helix | 1761-1762 | 2 | |
| β-strand | 1763 | 1 | 14 |
| α-helix | 1765-1772 | 8 | |
| β-strand | 1777-1781 | 5 | 15 |
| β-strand | 1788-1792 | 5 | 16 |
| β-strand | 1798-1802 | 5 | 16 |
| β-strand | 1805-1810 | 6 | 15 |
| β-strand | 1816-1822 | 7 | 15 |
| α-helix | 1824-1827 | 4 | |
| α-helix | 1837-1851 | 15 | |
| α-helix | 1854-1856 | 3 | |
| β-strand | 1860-1863 | 4 | 15 |
| α-helix | 1866-1868 | 3 | |
| α-helix | 1869-1875 | 7 | |
| β-strand | 1883-1886 | 4 | 15 |
| β-strand | 1887 | 1 | 14 |
| α-helix | 1892-1898 | 7 | |
| α-helix | 1900-1908 | 9 | |
| β-strand | 1914-1918 | 5 | 15 |
| α-helix | 1923-1925 | 3 | |
| α-helix | 1929-1946 | 18 | |
| α-helix | 1948-1954 | 7 | |
| α-helix | 1973-1995 | 23 | |
| α-helix | 2004-2012 | 9 | |
Chain b: 4 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-10 | 4 | |
| β-strand | 16-20 | 5 | 71 |
| β-strand | 26-34 | 9 | 71 |
| β-strand | 40-49 | 10 | 71 |
| β-strand | 63-67 | 5 | 71 |
| β-strand | 70-72 | 3 | 71 |
| α-helix | 74-76 | 3 | |
| β-strand | 79-82 | 4 | 71 |
| α-helix | 100-102 | 3 | |
| β-strand | 107-109 | 3 | 43 |
| α-helix | 114-116 | 3 | |
Chain c: 4 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-9 | 7 | |
| β-strand | 14-19 | 6 | 71 |
| β-strand | 24-29 | 6 | 71 |
| β-strand | 38-46 | 9 | 71 |
| α-helix | 51-52 | 2 | |
| β-strand | 53-60 | 8 | 71 |
| α-helix | 62-64 | 3 | |
| β-strand | 65-69 | 5 | 71 |
| α-helix | 76-80 | 5 | |
Chain C: 46 helices, 43 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 60-62 | 3 | |
| α-helix | 69-73 | 5 | |
| β-strand | 78-81 | 4 | 18 |
| β-strand | 103 | 1 | 19 |
| α-helix | 109-111 | 3 | |
| α-helix | 116-124 | 9 | |
| β-strand | 129-135 | 7 | 20 |
| α-helix | 142-153 | 12 | |
| β-strand | 171 | 1 | 19 |
| α-helix | 172-177 | 6 | |
| β-strand | 184-191 | 8 | 20 |
| β-strand | 197-204 | 8 | 20 |
| α-helix | 210-212 | 3 | |
| α-helix | 213-221 | 9 | |
| β-strand | 224-230 | 7 | 20 |
| α-helix | 237-248 | 12 | |
| β-strand | 252-258 | 7 | 20 |
| α-helix | 261-262 | 2 | |
| α-helix | 263-267 | 5 | |
| α-helix | 271-292 | 22 | |
| α-helix | 302-304 | 3 | |
| β-strand | 307-311 | 5 | 20 |
| β-strand | 316-318 | 3 | 20 |
| α-helix | 320-331 | 12 | |
| α-helix | 336-340 | 5 | |
| β-strand | 348-350 | 3 | 21 |
| β-strand | 355-357 | 3 | 21 |
| β-strand | 367 | 1 | 21 |
| α-helix | 368-369 | 2 | |
| α-helix | 370-374 | 5 | |
| α-helix | 375-384 | 10 | |
| α-helix | 392-398 | 7 | |
| α-helix | 407-409 | 3 | |
| α-helix | 412-424 | 13 | |
| α-helix | 428-437 | 10 | |
| α-helix | 439-440 | 2 | |
| α-helix | 441-452 | 12 | |
| β-strand | 453 | 1 | 22 |
| α-helix | 460-466 | 7 | |
| β-strand | 475-483 | 9 | 23 |
| β-strand | 490-498 | 9 | 23 |
| β-strand | 501-502 | 2 | 24 |
| β-strand | 506-511 | 6 | 23 |
| β-strand | 515 | 1 | 25 |
| β-strand | 518 | 1 | 25 |
| β-strand | 522-526 | 5 | 23 |
| β-strand | 530-533 | 4 | 23 |
| β-strand | 538-541 | 4 | 23 |
| β-strand | 543-544 | 2 | 24 |
| β-strand | 549-552 | 4 | 23 |
| β-strand | 563-566 | 4 | 23 |
| α-helix | 576 | 1 | |
| β-strand | 577 | 1 | 22 |
| α-helix | 578-580 | 3 | |
| β-strand | 588-594 | 7 | 26 |
| α-helix | 597-599 | 3 | |
| α-helix | 600-613 | 14 | |
| β-strand | 618-621 | 4 | 26 |
| β-strand | 627-631 | 5 | 26 |
| α-helix | 634-642 | 9 | |
| α-helix | 643-649 | 7 | |
| β-strand | 653-655 | 3 | 26 |
| α-helix | 656-658 | 3 | |
| β-strand | 659-660 | 2 | 26 |
| β-strand | 663-666 | 4 | 27 |
| β-strand | 674-677 | 4 | 28 |
| β-strand | 684-691 | 8 | 28 |
| α-helix | 696-701 | 6 | |
| α-helix | 711-722 | 12 | |
| α-helix | 726-730 | 5 | |
| β-strand | 732-736 | 5 | 28 |
| β-strand | 743-747 | 5 | 28 |
| α-helix | 756-776 | 21 | |
| β-strand | 784 | 1 | 27 |
| β-strand | 787-795 | 9 | 28 |
| α-helix | 808-822 | 15 | |
| β-strand | 825-838 | 14 | 27 |
| α-helix | 840-842 | 3 | |
| α-helix | 843-852 | 10 | |
| β-strand | 856-862 | 7 | 27 |
| α-helix | 863 | 1 | |
| β-strand | 869-876 | 8 | 27 |
| α-helix | 883-890 | 8 | |
| β-strand | 896-906 | 11 | 27 |
| α-helix | 907 | 1 | |
| α-helix | 926-928 | 3 | |
| α-helix | 929-941 | 13 | |
| α-helix | 949-951 | 3 | |
| α-helix | 955-962 | 8 | |
Chain d: 5 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-26 | 8 | |
| α-helix | 30-38 | 9 | |
| β-strand | 42-46 | 5 | 99 |
| β-strand | 51-54 | 4 | 99 |
| β-strand | 59 | 1 | 99 |
| β-strand | 65-73 | 9 | 99 |
| β-strand | 74 | 1 | 100 |
| α-helix | 79-81 | 3 | |
| α-helix | 85-87 | 3 | |
| β-strand | 92 | 1 | 100 |
| β-strand | 95-101 | 7 | 99 |
| α-helix | 103-105 | 3 | |
| β-strand | 106-109 | 4 | 99 |
Chain D: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 149-154 | 6 | |
| α-helix | 157-160 | 4 | |
| α-helix | 162-164 | 3 | |
| α-helix | 170-197 | 28 | |
28 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| U2 snRNA | 2 | RNA | 188 | Homo sapiens | |
| U6 snRNA | 6 | RNA | 106 | Homo sapiens | |
| 116 kDa U5 small nuclear ribonucleoprotein component | C | protein | 972 | Homo sapiens | Q15029 (AlphaFold model) |
| Pre-mRNA-splicing factor ISY1 homolog | D | protein | 285 | Homo sapiens | Q9ULR0 (AlphaFold model) |
| ATP-dependent RNA helicase DHX15 | DX | protein | 795 | Homo sapiens | O43143 (AlphaFold model) |
| U5 small nuclear ribonucleoprotein 40 kDa protein | E | protein | 357 | Homo sapiens | Q96DI7 (AlphaFold model) |
| Crooked neck-like protein 1 | J | protein | 848 | Homo sapiens | Q9BZJ0 |
| Pre-mRNA-splicing factor SPF27 | K | protein | 225 | Homo sapiens | O75934 |
| CWF19-like protein 1 | L1 | protein | 538 | Homo sapiens | Q69YN2 |
| Protein BUD31 homolog | N | protein | 144 | Homo sapiens | P41223 |
| Pre-mRNA-splicing factor RBM22 | O | protein | 420 | Homo sapiens | Q9NW64 |
| Intron-binding protein aquarius | Q | protein | 1485 | Homo sapiens | O60306 |
25 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>8RO2_1 U2 snRNA (chains 2)
AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAU
CUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGA
AUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACG
GUGCACCC
Sequence of entity 2 (6), FASTA
>8RO2_2 U6 snRNA (chains 6)
GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGC
CCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU
Sequence of entity 3 (C), FASTA
>8RO2_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM
Sequence of entity 4 (D), FASTA
>8RO2_4 Pre-mRNA-splicing factor ISY1 homolog (chains D)
MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQI
QNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGY
KYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKK
LRAELVEKWKAEREARLARGEKEEEEEEEEEINIYAVTEEESDEEGSQEKGGDDSQQKFI
AHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLGY
Sequence of entity 5 (DX), FASTA
>8RO2_5 ATP-dependent RNA helicase DHX15 (chains DX)
MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKE
LRASTNAMLISAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTN
LPHTPRYYDILKKRLQLPVWEYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRS
LPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGM
LLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKF
QIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQE
EIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS
TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGK
CFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRAL
ELLNYLAALNDDGDLTELGSMMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCF
VRPTEAKKAADEAKMRFAHIDGDHLTLLNVYHAFKQNHESVQWCYDNFINYRSLMSADNV
RQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQVAHLERTGHYLTVKDNQVVQ
LHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSNFPQCEAKRQL
DRIIAKLQSKEYSQY
Sequence of entity 6 (E), FASTA
>8RO2_6 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ
Sequence of entity 7 (J), FASTA
>8RO2_7 Crooked neck-like protein 1 (chains J)
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL
DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ
VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK
NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT
VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD
RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD
RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY
EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY
ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI
GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR
AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL
SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE
KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
EDVDESES
Sequence of entity 8 (K), FASTA
>8RO2_8 Pre-mRNA-splicing factor SPF27 (chains K)
MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSA
FETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVR
IENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLRE
MESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF
Sequence of entity 9 (L1), FASTA
>8RO2_9 CWF19-like protein 1 (chains L1)
MAQKPLRLLACGDVEGKFDILFNRVQAIQKKSGNFDLLLCVGNFFGSTQDAEWEEYKTGI
KKAPIQTYVLGANNQETVKYFQDADGCELAENITYLGRKGIFTGSSGLQIVYLSGTESLN
EPVPGYSFSPKDVSSLRMMLCTTSQFKGVDILLTSPWPKCVGNFGNSSGEVDTKKCGSAL
VSSLATGLKPRYHFAALEKTYYERLPYRNHIILQENAQHATRFIALANVGNPEKKKYLYA
FSIVPMKLMDAAELVKQPPDVTENPYRKSGQEASIGKQILAPVEESACQFFFDLNEKQGR
KRSSTGRDSKSSPHPKQPRKPPQPPGPCWFCLASPEVEKHLVVNIGTHCYLALAKGGLSD
DHVLILPIGHYQSVVELSAEVVEEVEKYKATLRRFFKSRGKWCVVFERNYKSHHLQLQVI
PVPISCSTTDDIKDAFITQAQEQQIELLEIPEHSDIKQIAQPGAAYFYVELDTGEKLFHR
IKKNFPLQFGREVLASEAILNVPDKSDWRQCQISKEDEETLARRFRKDFEPYDFTLDD
Sequence of entity 10 (N), FASTA
>8RO2_10 Protein BUD31 homolog (chains N)
MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYI
FDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICR
VPKSKLEVGRIIECTHCGCRGCSG
Sequence of entity 11 (O), FASTA
>8RO2_11 Pre-mRNA-splicing factor RBM22 (chains O)
MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPG
VRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQN
MEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECP
YRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLG
DTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNV
KWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAV
VNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP
Sequence of entity 12 (Q), FASTA
>8RO2_12 Intron-binding protein aquarius (chains Q)
MAAPAQPKKIVAPTVSQINAEFVTQLACKYWAPHIKKKSPFDIKVIEDIYEKEIVKSRFA
IRKIMLLEFSQYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWEIFKKKPDHFP
FFFKHILKAALAETDGEFSLHEQTVLLLFLDHCFNSLEVDLIRSQVQQLISLPMWMGLQL
ARLELELKKTPKLRKFWNLIKKNDEKMDPEAREQAYQERRFLSQLIQKFISVLKSVPLSE
PVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLLVHCYLSNLVRREEDGHLFSQ
LLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQRAAFAHFPELYDFALSNVAEVD
TRESLVKFFGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQIQQ
LNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLRNFNLFRLESTYE
IRQDIEDSVSRMKPWQSEYGGVVFGGWARMAQPIVAFTVVEVAKPNIGENWPTRVRADVT
INLNVRDHIKDEWEGLRKHDVCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGM
LDDKGRVIEDGPEPRPNLRGESRTFRVFLDPNQYQQDMTNTIQNGAEDVYETFNIIMRRK
PKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSAHYSKMPNQIATLDFNDTFLS
IEHLKASFPGHNVKVTVEDPALQIPPFRITFPVRSGKGKKRKDADVEDEDTEEAKTLIVE
PHVIPNRGPYPYNQPKRNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNI
YHNFPEQRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHGEEELETEKDFSRYGRVNY
VLARRIELLEEVKRLQKSLGVPGDASYTCETAGYFFLYQVMSRWEEYISKVKNKGSTLPD
VTEVSTFFPFHEYFANAPQPIFKGRSYEEDMEIAEGCFRHIKKIFTQLEEFRASELLRSG
LDRSKYLLVKEAKIIAMTCTHAALKRHDLVKLGFKYDNILMEEAAQILEIETFIPLLLQN
PQDGFSRLKRWIMIGDHHQLPPVIKNMAFQKYSNMEQSLFTRFVRVGVPTVDLDAQGRAR
ASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQN
LGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTV
DRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFARVSLFQNCFELTPAF
SQLTARPLHLHIIPTEPFPTTRKNGERPSHEVQIIKNMPQMANFVYNMYMHLIQTTHHYH
QTLLQLPPAMVEEGEEVQNQETELETEEEAMTVQADIIPSPTDTSCRQETPAFQTDTTPS
ETGATSTPEAIPALSETTPTVVGAVSAPAEANTPQDATSAPEETK
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| GTP | Guanosine-5'-triphosphate | C10 H16 N5 O14 P3 | 1 |
| ZN | Zinc ion | Zn | 3 |
| IHP | Inositol hexakisphosphate | C6 H18 O24 P6 | 1 |
Primary citation
Mechanism for the initiation of spliceosome disassembly. Vorlander, M.K., Rothe, P., Kleifeld, J. et al. Nature (2024) 632:443-450. DOI 10.1038/s41586-024-07741-1 · PubMed
Other PDB entries of the same protein (UniProt Q15029 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9S2E 2.4 Å, Human intron-lariat spliceosome-Aquarius (ILSaqr)
- 8H6L 2.6 Å, Cryo-EM structure of human exon-defined spliceosome in the early B state.
- 8H6K 2.7 Å, Cryo-EM structure of human exon-defined spliceosome in the mature B state.
- 8C6J 2.8 Å, Human spliceosomal PM5 C* complex
- 6ID1 2.86 Å, Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex)…
- 7DVQ 2.89 Å, Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
- 6ID0 2.9 Å, Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1…
- 6QW6 2.92 Å, Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.
- 9XTT 2.92 Å, Human minor spliceosome branching-completed C complex (after step-I)
- 6ICZ 3.0 Å, Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
- 8I0R 3.0 Å, The cryo-EM structure of human Bact-I complex
- 8I0T 3.0 Å, The cryo-EM structure of human Bact-III complex
Browse structure collections
About this viewer
MolViewer shows 8RO2 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.