8SH1: Human POT1 DNA binding domain

Structure of human POT1 DNA binding domain bound to a 5'-phosphorylated junction of a telomeric double-stranded DNA duplex with a 3'-overhang. Determined by X-ray diffraction at 2.6 Å resolution. Released 30 Aug 2023.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Homo sapiens
Chains: 3
Atoms: 3,042
Mol. weight: 43.72 kDa
Released: 30 Aug 2023

Explore 8SH1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8SH1 contains 13 α-helices and 17 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	11	1	1
α-helix	14-16	3
β-strand	21-32	12	2
α-helix	33-35	3
β-strand	36-37	2	2
β-strand	43-50	8	2
β-strand	56-63	8	2
α-helix	66-68	3
β-strand	78-89	12	2
β-strand	92-106	15	2
β-strand	117	1	2
α-helix	127-143	17
β-strand	150	1	3
α-helix	153-155	3
β-strand	160-173	14	4
β-strand	178-184	7	4
α-helix	192	1
β-strand	193	1	4
α-helix	194	1
β-strand	204	1	4
α-helix	207-212	6
β-strand	218-223	6	4
α-helix	225-231	7
α-helix	233-234	2
β-strand	238-251	14	4
β-strand	257-265	9	4
α-helix	270-272	3
β-strand	274-278	5	4
α-helix	279	1
α-helix	283-293	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protection of telomeres protein 1	A	protein	300	Homo sapiens	Q9NUX5 (AlphaFold model)
DNA (5'-d(cpgpcpgpcpgptptpapgpgpgptptpapgpgpgptptpapg)-3')	B	DNA	22	Homo sapiens
DNA (5'-d(pcptpapapcpgpcpgpcpg)-3')	C	DNA	10	Homo sapiens

Sequence of entity 1 (A), FASTA

>8SH1_1 Protection of telomeres protein 1 (chains A)
SMSLVPATNYIYTPLNQLKGGTIVNVYGVVKFFKPPYLSKGTDYCSVVTIVDQTNVKLTC
LLFSGNYEALPIIYKNGDIVRFHRLKIQVYKKETQGITSSGFASLTFEGTLGAPIIPRTS
SKYFNFTTEDHKMVEALRVWASTHMSPSWTLLKLCDVQPMQYFDLTCQLLGKAEVDGASF
LLKVWDGTRTPFPSWRVLIQDLVLEGDLSHIHRLQNLTIDILVYDNHVHVARSLKVGSFL
RIYSLHTKLQSMNSENQTMLSLEFHLHGGTSYGRGIRVLPESNSDVDQLKKDLESANLTA

Sequence of entity 2 (B), FASTA

>8SH1_2 DNA (5'-D(*CP*GP*CP*GP*CP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') (chains B)
CGCGCGTTAGGGTTAGGGTTAG

Sequence of entity 3 (C), FASTA

>8SH1_3 DNA (5'-D(P*CP*TP*AP*AP*CP*GP*CP*GP*CP*G)-3') (chains C)
CTAACGCGCG

Primary citation

Human POT1 protects the telomeric ds-ss DNA junction by capping the 5' end of the chromosome. Tesmer, V.M., Brenner, K.A., Nandakumar, J. Science (2023) 381:771-778. DOI 10.1126/science.adi2436 · PubMed

Other PDB entries of the same protein (UniProt Q9NUX5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1XJV 1.73 Å, Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)
3KJO 1.8 Å, Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)
3KJP 1.8 Å, Crystal Structure of hPOT1V2-GGTTAGGGTTAG
5H65 2.1 Å, Crystal structure of human POT1 and TPP1
5UN7 2.1 Å, Structure of the human POT1-TPP1 telomeric complex
8SH0 2.16 Å, Structure of human POT1 DNA binding domain bound to a 5'-phosphorylated junction of a…
7S1O 2.55 Å, Structure of human POT1C
7S1U 2.55 Å, Structure of human POT1C
7S1T 2.9 Å, Structure of the human POT1-TPP1 complex
8SOJ 3.8 Å, Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the absence of telomeric ssDNA
7QXB 3.9 Å, Cryo-EM map of human telomerase-DNA-TPP1-POT1 complex (sharpened map)
7QXS 3.9 Å, Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains)

Browse structure collections

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