8T3Q: DHA bound FFA4-Gq complex

Cryo-EM structure of the DHA bound FFA4-Gq complex. Determined by electron microscopy at 3.14 Å resolution. Released 24 Jan 2024.

Method: Electron microscopy
Resolution: 3.14 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 9,035
Mol. weight: 134.08 kDa
Ligands: HXA
Released: 24 Jan 2024

Explore 8T3Q in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8T3Q contains 34 α-helices and 65 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-35	2	1
β-strand	38-39	2	2
α-helix	46-48	3
β-strand	72-76	5	1
β-strand	79-83	5	1
α-helix	96-98	3
β-strand	105-111	7	2
α-helix	118-129	12
β-strand	138-144	7	2
α-helix	146-155	10
α-helix	165-169	5
α-helix	172-173	2
α-helix	184-203	20
β-strand	215	1	2
α-helix	224-242	19

Chain B: 6 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	45-51	7	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
α-helix	95	1
β-strand	102-105	4	5
β-strand	111-115	5	5
β-strand	121-125	5	5
β-strand	134-139	6	5
α-helix	145	1
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-307	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-341	6	3

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	27-29	3
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	11-12	2	11
β-strand	17-25	9	10
α-helix	29-31	3
β-strand	34-39	6	12
β-strand	45-51	7	12
α-helix	53-55	3
β-strand	58-60	3	12
β-strand	68-73	6	10
β-strand	78-84	7	10
β-strand	93-97	5	12
β-strand	115	1	12
β-strand	118-119	2	11
β-strand	141-142	2	13
β-strand	148-149	2	14
β-strand	156-160	5	15
β-strand	161-162	2	13
β-strand	167	1	16
β-strand	173	1	16
β-strand	175-180	6	14
β-strand	187-191	5	14
β-strand	195-196	2	14
α-helix	197	1
β-strand	198	1	17
β-strand	200	1	17
β-strand	204-207	4	15
β-strand	213-217	5	15
β-strand	226-232	7	14
β-strand	244-247	4	14

Chain R: 12 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	36-70	35
α-helix	78-88	11
α-helix	92-101	10
α-helix	108-139	32
α-helix	152-169	18
α-helix	172-175	4
β-strand	177	1	18
β-strand	180-181	2	19
β-strand	192-193	2	19
β-strand	196	1	18
α-helix	201-212	12
α-helix	213-217	5
α-helix	218-244	27
α-helix	249-255	7
α-helix	259-290	32
α-helix	300-321	22

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q)	A	protein	230	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	342	Homo sapiens	P62873 (AlphaFold model)
scFv16	N	protein	266	Mus musculus
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	57	Homo sapiens	P59768 (AlphaFold model)
Free fatty acid receptor 4	R	protein	308	Homo sapiens	Q5NUL3 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8T3Q_1 Guanine nucleotide-binding protein G(q) (chains A)
VSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMTSGIFETKFQV
DKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRT
ISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRKE
FVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLREYNLV

Sequence of entity 2 (B), FASTA

>8T3Q_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSS

Sequence of entity 3 (N), FASTA

>8T3Q_3 scFv16 (chains N)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELLEENLYFQGASHHHHHHHH

Sequence of entity 4 (G), FASTA

>8T3Q_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
TASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFR

Sequence of entity 5 (R), FASTA

>8T3Q_5 Free fatty acid receptor 4 (chains R)
RTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVCALVLVARRRRRGATACLVLNL
FCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTLSGSVTILTLAAVSLERMVCIV
HLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVVPQRLPGADQEISICTLIWPTI
PGEISWDVSFVTLNFLVPGLVIVISYSKILQITKASRKRLTVSLAYSESHQIRVSQQDFR
LFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWPSLFFWVVAFTFANSALNPILY
NMTLCRNE

Ligands and cofactors

ID	Name	Formula	Copies
HXA	Docosa-4,7,10,13,16,19-hexaenoic acid	C22 H32 O2	1

Primary citation

Structural basis for the ligand recognition and signaling of free fatty acid receptors. Zhang, X., Guseinov, A.A., Jenkins, L. et al. Sci Adv (2024) 10:eadj2384-eadj2384. DOI 10.1126/sciadv.adj2384 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

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