8UTW: KIF1A[1-393] P305L mutant APO

KIF1A[1-393] P305L mutant APO in complex with a microtubule. Determined by electron microscopy at 3.4 Å resolution. Released 12 Jun 2024.

Method: Electron microscopy
Resolution: 3.4 Å
Organisms: Homo sapiens, Sus scrofa
Chains: 3
Atoms: 9,787
Mol. weight: 151.35 kDa
Ligands: TA1, GDP, MG, GTP
Released: 12 Jun 2024

Explore 8UTW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8UTW contains 58 α-helices and 54 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 21 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	6-9	4	7
α-helix	10-28	19
α-helix	37-42	6
β-strand	53-55	3	8
β-strand	61-63	3	8
β-strand	65-68	4	7
β-strand	69	1	9
α-helix	72-79	8
β-strand	93	1	9
α-helix	103-108	6
α-helix	115-127	13
β-strand	134-140	7	7
α-helix	145-160	16
β-strand	165-172	8	7
α-helix	183-197	15
β-strand	200-205	6	7
α-helix	206-215	10
α-helix	224-239	16
α-helix	240-242	3
β-strand	248	1	10
α-helix	252-258	7
β-strand	271-272	2	10
α-helix	288-295	8
α-helix	298-300	3
α-helix	307-309	3
β-strand	312-321	10	10
α-helix	325-337	13
β-strand	343	1	10
β-strand	352	1	10
β-strand	355-356	2	10
α-helix	359-361	3
α-helix	368-370	3
β-strand	373-381	9	10
α-helix	384-398	15
α-helix	405-409	5
α-helix	416-435	20
α-helix	438-439	2

Chain B: 23 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	2-9	8	11
α-helix	10-28	19
β-strand	30	1	12
β-strand	36	1	12
α-helix	41-49	9
β-strand	51-53	3	13
β-strand	59-61	3	13
β-strand	64-67	4	11
α-helix	70-77	8
α-helix	82-84	3
α-helix	87-89	3
β-strand	91-92	2	11
α-helix	101-102	2
α-helix	103-107	5
α-helix	109-126	18
β-strand	129-138	10	11
α-helix	144-158	15
β-strand	163-170	8	11
α-helix	171-172	2
α-helix	181-193	13
β-strand	198-203	6	11
α-helix	204-209	6
α-helix	210-214	5
α-helix	222-237	16
α-helix	238-240	3
β-strand	246	1	14
α-helix	250-257	8
β-strand	265-266	2	11
β-strand	267-271	5	14
α-helix	286-293	8
β-strand	310	1	15
β-strand	312-319	8	14
α-helix	323-336	14
α-helix	338-340	3
β-strand	341	1	15
β-strand	350-354	5	14
β-strand	363-370	8	14
α-helix	372-374	3
α-helix	375-389	15
α-helix	396-399	4
α-helix	406-424	19

Chain K: 14 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	5-12	8	1
α-helix	17-21	5
β-strand	26	1	2
β-strand	28-31	4	3
β-strand	34-37	4	3
β-strand	48-51	4	3
β-strand	54-56	3	1
α-helix	70-73	4
α-helix	74-78	5
α-helix	79-86	8
β-strand	91-96	6	1
α-helix	103-107	5
β-strand	109	1	4
β-strand	116	1	4
α-helix	118-133	16
β-strand	138-150	13	1
β-strand	153-156	4	1
α-helix	164-166	3
β-strand	168-171	4	5
β-strand	175-178	4	5
β-strand	184-185	2	1
α-helix	189-201	13
β-strand	205-206	2	6
β-strand	214-215	2	6
β-strand	218-229	12	1
β-strand	241-248	8	1
α-helix	249-252	4
α-helix	263-290	28
α-helix	291-295	5
α-helix	310-314	5
α-helix	316-319	4
β-strand	324-331	8	1
β-strand	334	1	2
α-helix	338-349	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinesin-like protein KIF1A	K	protein	438	Homo sapiens	Q12756 (AlphaFold model)
Tubulin alpha-1B chain	A	protein	451	Sus scrofa	Q2XVP4 (AlphaFold model)
Tubulin beta-2B chain	B	protein	445	Sus scrofa	A0A8D1UIR5 (AlphaFold model)

Sequence of entity 1 (K), FASTA

>8UTW_1 Kinesin-like protein KIF1A (chains K)
MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHT
SPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIP
QLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVED
LSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEK
VSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKK
TDFILYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVI
NEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDGAGVKQLEDKVEELASKNYHLENEVAR
LKKLVEFTSAWSHPQFEK

Sequence of entity 2 (A), FASTA

>8UTW_2 Tubulin alpha-1B chain (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Sequence of entity 3 (B), FASTA

>8UTW_3 Tubulin beta-2B chain (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

Ligands and cofactors

ID	Name	Formula	Copies
TA1	Taxol	C47 H51 N O14	1
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1
MG	Magnesium ion	Mg	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1

Primary citation

Cryo-EM unveils kinesin KIF1A's processivity mechanism and the impact of its pathogenic variant P305L. Benoit, M.P.M.H., Rao, L., Asenjo, A.B. et al. Nat Commun (2024) 15:5530-5530. DOI 10.1038/s41467-024-48720-4 · PubMed

Other PDB entries of the same protein (UniProt Q12756 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4EJQ 1.89 Å, Crystal structure of KIF1A C-CC1-FHA
4EGX 2.51 Å, Crystal structure of KIF1A CC1-FHA tandem
8UTS 2.7 Å, KIF1A[1-393] APO in complex with a microtubule
9YA5 2.95 Å, KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP
8UTU 3.0 Å, KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in…
8UTV 3.0 Å, KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule
8UTN 3.1 Å, KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class…
8UTQ 3.1 Å, KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class…
8UTT 3.1 Å, KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a…
9YAI 3.12 Å, KIF1A R350W bound to microtubules in two-heads-bound state with AMP-PNP
8UTO 3.2 Å, KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class…
8UTP 3.2 Å, KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1

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