8V6N: PDB entry 8V6N

Open-state cryo-EM structure of human TRPV3 in presence of 2-APB in cNW30 nanodiscs. Determined by electron microscopy at 2.59 Å resolution. Released 17 Apr 2024.

Method

Electron microscopy

Resolution

2.59 Å

Organism

Homo sapiens

Chains

4

Atoms

22,185

Mol. weight

388.96 kDa

Ligands

FZ4, ACD, POV

Released

17 Apr 2024

Explore 8V6N in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8V6N contains 140 α-helices and 52 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 35 helices, 13 β-strands

Molecules and chains

>8V6N_1 Transient receptor potential cation channel subfamily V member 3 (chains A, B, C, D)
MKAHPKEMVPLMGKRVAAPSGNPAILPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTS
PPVFSKPMDSNIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKRRLK
KRIFAAVSEGCVEELVELLVELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNIN
PNTKEIVRILLAFAEENDILGRFINAEYTEEAYEGQTALNIAIERRQGDIAALLIAAGAD
VNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQLLMEHEQTDITSRDSRGNNIL
HALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAKMGKAEILKYI
LSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEML
TLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMG
WLQLLGRMFVLIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLY
LFAYKEYLACLVLAMALGWANMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLG
FGVALASLIEKCPKDNKDCSSYGSFSDAVLELFKLTIGLGDLNIQQNSKYPILFLFLLIT
YVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTILEFEKMLPEWLRSRFRMGEL
CKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTADFNKIQDSSRNNSKTTLNAF
EEVEEFPETSVLVPRGSAAAWSHPQFEK

Ligands and cofactors

Water and common crystallization additives (NA) are not listed.

Primary citation

TRPV3 activation by different agonists accompanied by lipid dissociation from the vanilloid site. Nadezhdin, K.D., Neuberger, A., Khosrof, L.S. et al. Sci Adv (2024) 10:eadn2453-eadn2453. DOI 10.1126/sciadv.adn2453 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q8NET8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 6H9J 1.83 Å, Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (229-255)
• 6HA6 1.98 Å, Factor Inhibiting HIF (FIH) in complex with zinc, NOG and TRPV3 (220-246)
• 7QQN 2.45 Å, The PDZ domain of SNTG1 complexed with the acetylated PDZ-binding motif of TRPV3
• 8V6K 2.46 Å, Apo-state cryo-EM structure of human TRPV3 in cNW30 nanodiscs
• 13LU 2.49 Å, Structure of human TRPV3-W521S Olmsted syndrome mutant in the closed state
• 7XJ0 2.53 Å, Structure of human TRPV3 in complex with Trpvicin
• 8GKA 2.55 Å, Human TRPV3 tetramer structure, closed conformation
• 9PJ5 2.76 Å, Closed-state structure of wild-type human TRPV3 purified in GDN detergent
• 8V6O 2.83 Å, Inactivated-state cryo-EM structure of human TRPV3 in presence of 2-APB in cNW30 nanodiscs
• 13LJ 2.86 Å, Structure of human TRPV3-G568C Olmsted syndrome mutant in the closed state
• 7XJ1 2.93 Å, Structure of human TRPV3_G573S in complex with Trpvicin in C2 symmetry
• 13LL 2.96 Å, Structure of human TRPV3-Q580P Olmsted syndrome mutant in the closed state

Browse structure collections

About this viewer

MolViewer shows 8V6N directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.