8W89: PEA-bound TAAR1-Gs complex
Cryo-EM structure of the PEA-bound TAAR1-Gs complex. Determined by electron microscopy at 3.0 Å resolution. Released 22 Nov 2023.
- Method
- Electron microscopy
- Resolution
- 3.0 Å
- Organisms
- Homo sapiens, Lama glama
- Chains
- 5
- Atoms
- 8,230
- Mol. weight
- 128.6 kDa
- Ligands
- PEA
- Released
- 22 Nov 2023
Explore 8W89 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8W89 contains 34 α-helices and 44 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-34 | 22 | |
| β-strand | 40-46 | 7 | 1 |
| α-helix | 53-55 | 3 | |
| β-strand | 69-75 | 7 | 1 |
| β-strand | 80-86 | 7 | 1 |
| α-helix | 99-101 | 3 | |
| β-strand | 105-111 | 7 | 1 |
| α-helix | 118-130 | 13 | |
| β-strand | 138-144 | 7 | 1 |
| α-helix | 146-155 | 10 | |
| α-helix | 160-163 | 4 | |
| α-helix | 165-169 | 5 | |
| α-helix | 184-203 | 20 | |
| β-strand | 211-215 | 5 | 1 |
| α-helix | 223-242 | 20 | |
Chain B: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 120-125 | 6 | 4 |
| β-strand | 134-140 | 7 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 165-170 | 6 | 5 |
| β-strand | 175-181 | 7 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 208-212 | 5 | 6 |
| β-strand | 218-222 | 5 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 260-264 | 5 | 7 |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 303-307 | 5 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-23 | 17 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain N: 1 helix, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-6 | 4 | 9 |
| β-strand | 10-12 | 3 | 10 |
| β-strand | 18-25 | 8 | 9 |
| β-strand | 34-39 | 6 | 10 |
| β-strand | 45-51 | 7 | 10 |
| β-strand | 58-60 | 3 | 10 |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-83 | 6 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 10 |
| β-strand | 122-126 | 5 | 10 |
Chain R: 18 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 20-51 | 32 | |
| α-helix | 57-71 | 15 | |
| α-helix | 72-76 | 5 | |
| α-helix | 77-84 | 8 | |
| α-helix | 93-126 | 34 | |
| α-helix | 131-134 | 4 | |
| α-helix | 137-156 | 20 | |
| α-helix | 157-161 | 5 | |
| α-helix | 169-179 | 11 | |
| α-helix | 183-185 | 3 | |
| α-helix | 188-195 | 8 | |
| α-helix | 196-200 | 5 | |
| α-helix | 201-230 | 30 | |
| α-helix | 248-273 | 26 | |
| α-helix | 276-278 | 3 | |
| α-helix | 283-301 | 19 | |
| α-helix | 302-306 | 5 | |
| α-helix | 309-315 | 7 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 246 | Homo sapiens | P04899 (AlphaFold model), P63092 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 345 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Nanobody35 | N | protein | 139 | Lama glama | |
| Trace amine-associated receptor 1 | R | protein | 339 | Homo sapiens | Q96RJ0 |
Sequence of entity 1 (A), FASTA
>8W89_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
GGSGGSMGSTVSAEDKAAAERSKMIDKNLREDGEKAAAATHRLLLLGADNSGKSTIVKQM
RIYHVNSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHL
RQYELL
Sequence of entity 2 (B), FASTA
>8W89_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (G), FASTA
>8W89_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (N), FASTA
>8W89_4 Nanobody35 (chains N)
MQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASIS
YTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRG
QGTQVTVSSHHHHHHEPEA
Sequence of entity 5 (R), FASTA
>8W89_5 Trace amine-associated receptor 1 (chains R)
MMPFCHNIINISCVKNNWSNDVRASLYSLMVLIILTTLVGNLIVIVSISHFKQLHTPTNW
LIHSMATVDFLLGCLVMPYSMVRSAEHCWYFGEVFCKIHTSTDIMLSSASIFHLSFISID
RYYAVCDPLRYKAKMNILVICVMIFISWSVPAVFAFGMIFLELNFKGAEEIYYKHVHCRG
GCSVFFSKISGVLTFMTSFYIPGSIMLCVYYRIYLIAKEQARLISDANQKLQIGLEMKNG
ISQSKERKAVKTLGIVMGVFLICWCPFFICTVMDPFLHYIIPPTLNDVLIWFGYLNSTFN
PMVYAFFYPWFRKALKMMLFGKIFQKDSSRCKLFLELSS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| PEA | 2-phenylethylamine | C8 H12 N | 1 |
Primary citation
Recognition of methamphetamine and other amines by trace amine receptor TAAR1. Liu, H., Zheng, Y., Wang, Y. et al. Nature (2023) 624:663-671. DOI 10.1038/s41586-023-06775-1 · PubMed
Other PDB entries of the same protein (UniProt P04899 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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- 9XDQ 2.79 Å, Cryo-EM structure of BMS986187 bound DOR-Gi complex
- 7WVX 2.8 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fhumanin and Gi2
- 8W8A 2.8 Å, Cryo-EM structure of the RO5256390-TAAR1 complex
- 8ZSJ 2.8 Å, Cryo-EM structure of the apo hTAAR1-Gs complex
- 7WVV 2.9 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fMLFII and Gi2
- 9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
- 9KFI 2.91 Å, Cryo-EM structure of the human relaxin family peptide receptor 3 in complex with…
- 9KFK 2.95 Å, Cryo-EM structure of the relaxin-3-bound human relaxin family peptide receptor 4…
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