8W8X: Beta-MSH-MC3R-Gs_Nb35 complex
Cryo-EM structure of beta-MSH-MC3R-Gs_Nb35 complex. Determined by electron microscopy at 2.9 Å resolution. Released 19 Mar 2025.
- Method
- Electron microscopy
- Resolution
- 2.9 Å
- Organisms
- Rattus norvegicus, Bos taurus, Lama glama
- Chains
- 6
- Atoms
- 7,608
- Mol. weight
- 180.57 kDa
- Ligands
- CA
- Released
- 19 Mar 2025
Explore 8W8X in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8W8X contains 24 α-helices and 45 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 6 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-38 | 23 | |
| β-strand | 40-44 | 5 | 10 |
| β-strand | 209-214 | 6 | 10 |
| β-strand | 217-222 | 6 | 10 |
| α-helix | 235-238 | 4 | |
| β-strand | 243-248 | 6 | 10 |
| α-helix | 265-277 | 13 | |
| β-strand | 286-291 | 6 | 10 |
| α-helix | 313-317 | 5 | |
| α-helix | 332-349 | 18 | |
| β-strand | 359-363 | 5 | 10 |
| α-helix | 371-390 | 20 | |
Chain B: 2 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 30-33 | 4 | |
| β-strand | 47-51 | 5 | 1 |
| β-strand | 58-63 | 6 | 2 |
| β-strand | 69-74 | 6 | 2 |
| β-strand | 78-83 | 6 | 2 |
| β-strand | 91-94 | 4 | 2 |
| β-strand | 100-105 | 6 | 3 |
| β-strand | 111-116 | 6 | 3 |
| β-strand | 121-125 | 5 | 3 |
| β-strand | 134-139 | 6 | 3 |
| β-strand | 146-153 | 8 | 4 |
| β-strand | 156-161 | 6 | 4 |
| β-strand | 166-170 | 5 | 4 |
| β-strand | 175-180 | 6 | 4 |
| β-strand | 187-192 | 6 | 5 |
| β-strand | 198-203 | 6 | 5 |
| β-strand | 208-212 | 5 | 5 |
| β-strand | 217-222 | 6 | 5 |
| β-strand | 229-234 | 6 | 6 |
| β-strand | 240-245 | 6 | 6 |
| β-strand | 250-254 | 5 | 6 |
| β-strand | 259-264 | 6 | 6 |
| β-strand | 273-278 | 6 | 7 |
| β-strand | 284-289 | 6 | 7 |
| β-strand | 294-298 | 5 | 7 |
| β-strand | 304-308 | 5 | 7 |
| β-strand | 315-320 | 6 | 1 |
| β-strand | 327-331 | 5 | 1 |
| β-strand | 336-339 | 4 | 1 |
Chain G: 5 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-23 | 14 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
| α-helix | 56-58 | 3 | |
Chain N: 2 helices, 11 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 8 |
| β-strand | 10-11 | 2 | 9 |
| β-strand | 17-25 | 9 | 8 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 9 |
| β-strand | 45-51 | 7 | 9 |
| β-strand | 58-60 | 3 | 9 |
| β-strand | 68-73 | 6 | 8 |
| β-strand | 78-84 | 7 | 8 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 9 |
| β-strand | 118 | 1 | 9 |
| β-strand | 122-125 | 4 | 9 |
Chain R: 9 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 42-65 | 24 | |
| α-helix | 72-100 | 29 | |
| α-helix | 111-147 | 37 | |
| α-helix | 158-181 | 24 | |
| α-helix | 188-222 | 35 | |
| α-helix | 239-267 | 29 | |
| α-helix | 276-279 | 4 | |
| α-helix | 281-298 | 18 | |
| α-helix | 304-309 | 6 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 384 | Rattus norvegicus | P54311 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Bos taurus | P63212 (AlphaFold model) |
| Nanobody35 | N | protein | 160 | Lama glama | |
| Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | A | protein | 380 | Homo sapiens | P63092 (AlphaFold model) |
| Soluble cytochrome b562,Melanocortin receptor 3,Fusion protein | R | protein | 602 | Escherichia coli, Homo sapiens | P41968 (AlphaFold model) |
| Beta-MSH | L | protein | 18 | Homo sapiens | P01189 |
Sequence of entity 1 (B), FASTA
>8W8X_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGS
SGGGGSGGGGSSGVSGWRLFKKIS
Sequence of entity 2 (G), FASTA
>8W8X_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 3 (N), FASTA
>8W8X_3 Nanobody35 (chains N)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA
Sequence of entity 4 (A), FASTA
>8W8X_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL
Sequence of entity 5 (R), FASTA
>8W8X_5 Soluble cytochrome b562,Melanocortin receptor 3,Fusion protein (chains R)
DYKDDDDKGSADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLED
KSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLLVPR
GSNASCCLPSVQPTLPNGSEHLQAPFFSNQSSSAFCEQVFIKPEVFLSLGIVSLLENILV
ILAVVRNGNLHSPMYFFLCSLAVADMLVSVSNALETIMIAIVHSDYLTFEDQFIQHMDNI
FDSMICISLVASICNLLAIAVDRYVTIFYALRYHSIMTVRKALTLIVAIWVCCGVCGVVF
IVYSESKMVIVCLITMFFAMMLLMGTLYVHMFLFARLHVKRIAALPPADGVAPQQHSCMK
GAVTITILLGVFIFCWAPFFLHLVLIITCPTNPYCICYTAHFNTYLVLIMCNSVIDPLIY
AFRSLELRNTFREILCGCNGMNLGVFTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLA
VSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYG
TLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTI
NS
Sequence of entity 6 (L), FASTA
>8W8X_6 Beta-MSH (chains L)
DEGPYRMEHFRWGSPPKD
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 1 |
Primary citation
Structure basis of ligand selectivity and signal transduction in human melanocortin-3 receptor. Jingpeng, F., Hongli, H. To be published.
Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
- 9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
- 22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
- 7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
- 9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
- 8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
- 9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
- 8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
- 9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
Browse structure collections
About this viewer
MolViewer shows 8W8X directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.