8WGB: MGlu2-4 heterodimer

mGlu2-4 heterodimer bound with Gi. Determined by electron microscopy at 3.7 Å resolution. Released 30 Oct 2024.

Method: Electron microscopy
Resolution: 3.7 Å
Organism: Homo sapiens
Chains: 5
Atoms: 16,797
Mol. weight: 278.57 kDa
Ligands: W9R, GLU
Released: 30 Oct 2024

Explore 8WGB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8WGB contains 79 α-helices and 89 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 31 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	26-27	2	10
β-strand	34-38	5	10
β-strand	41	1	11
β-strand	53	1	11
α-helix	55-60	6
α-helix	61-74	14
β-strand	86-90	5	10
α-helix	95-106	12
β-strand	139-141	3	10
α-helix	145-155	11
α-helix	156-158	3
β-strand	162-164	3	12
α-helix	170-173	4
β-strand	181-183	3	12
α-helix	188-201	14
β-strand	206-212	7	13
α-helix	215-230	16
β-strand	234-241	8	13
α-helix	247-258	12
β-strand	265-268	4	13
α-helix	278-280	3
β-strand	292-293	2	13
β-strand	315-319	5	13
α-helix	325-332	8
α-helix	344-352	9
α-helix	378-399	22
α-helix	415-417	3
α-helix	418-423	6
β-strand	429	1	14
α-helix	430	1
β-strand	440	1	14
β-strand	453-459	7	13
β-strand	467-474	8	13
β-strand	478-480	3	13
α-helix	482-484	3
β-strand	508-511	4	15
β-strand	521-524	4	15
α-helix	525-526	2
β-strand	530-533	4	16
β-strand	536-538	3	16
β-strand	544-546	3	17
β-strand	553-555	3	17
α-helix	556-558	3
β-strand	559	1	18
α-helix	566-591	26
α-helix	596-599	4
α-helix	604-623	20
α-helix	631-660	30
α-helix	676-700	25
β-strand	708	1	18
β-strand	718	1	18
α-helix	724	1
α-helix	728-748	21
α-helix	755-757	3
α-helix	760-783	24
α-helix	787-805	19
α-helix	806-810	5
α-helix	811-819	9

Chain C: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-32	24
β-strand	34-40	7	19
α-helix	46-55	10
β-strand	185-189	5	19
β-strand	196-200	5	19
α-helix	208-211	4
β-strand	220-226	7	19
α-helix	227-231	5
α-helix	242-254	13
α-helix	262-263	2
β-strand	264-269	6	19
α-helix	271-278	8
α-helix	283-285	3
α-helix	296-309	14
β-strand	319-323	5	19
α-helix	331-350	20

Chain D: 6 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	58-63	6	2
β-strand	69-74	6	2
β-strand	79-83	5	2
β-strand	88-93	6	2
β-strand	103-105	3	3
β-strand	111-114	4	3
β-strand	120-121	2	4
β-strand	122-125	4	3
α-helix	133	1
β-strand	134-135	2	3
β-strand	139-140	2	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250	1	8
β-strand	251-254	4	7
β-strand	264	1	8
α-helix	272	1
β-strand	273-278	6	9
α-helix	279	1
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-307	4	9
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain E: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-25	18
α-helix	30-43	14

Chain R: 30 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	45	1	20
β-strand	49-53	5	20
β-strand	58	1	21
β-strand	70	1	21
α-helix	76-91	16
β-strand	101-105	5	20
α-helix	112-119	8
β-strand	150-154	5	20
α-helix	159-169	11
β-strand	176	1	20
β-strand	178	1	22
α-helix	184-187	4
β-strand	197	1	22
α-helix	202-215	14
β-strand	221-226	6	23
α-helix	232-243	12
β-strand	250-256	7	23
α-helix	264-274	11
β-strand	280-284	5	23
α-helix	287-299	13
β-strand	307-310	4	23
α-helix	325-328	4
β-strand	332-336	5	23
α-helix	342-349	8
α-helix	362-370	9
α-helix	406-427	22
α-helix	436-438	3
α-helix	443-452	10
β-strand	475-480	6	23
β-strand	489-495	7	23
β-strand	500-501	2	23
β-strand	528	1	24
β-strand	533	1	25
β-strand	540	1	25
β-strand	544	1	24
α-helix	559-561	3
β-strand	564-565	2	26
β-strand	574-575	2	26
α-helix	576-577	2
β-strand	578	1	27
α-helix	589-611	23
α-helix	616-620	5
α-helix	624-643	20
α-helix	650-684	35
α-helix	690-691	2
α-helix	699-720	22
α-helix	727-729	3
β-strand	742	1	27
α-helix	750-773	24
α-helix	774-776	3
α-helix	779-781	3
α-helix	784-802	19
α-helix	803-807	5
α-helix	808-810	3
α-helix	817-847	31

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	D	protein	339	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Homo sapiens	P59768 (AlphaFold model)
Metabotropic glutamate receptor 2	B	protein	854	Homo sapiens	Q14416 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-3	C	protein	354	Homo sapiens	P08754 (AlphaFold model)
Metabotropic glutamate receptor 4	R	protein	880	Homo sapiens	Q14833

Sequence of entity 1 (D), FASTA

>8WGB_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 2 (E), FASTA

>8WGB_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (B), FASTA

>8WGB_3 Metabotropic glutamate receptor 2 (chains B)
EGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFALDRINRDPHL
LPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAITG
VIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEI
LRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALL
QKPSARVAVLFTRSEDARELLAASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITIE
LASYPISDFASYFQSLDPWNNSRNPWFREFWEQRFRCSFRQRDCAAHSLRAVPFEQESKI
MFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADTH
NEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSLIPWASPSAGPLPAS
RCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQ
EYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYC
MTFIFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPAS
QVAICLALISGQLLIVVAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLL
IALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSV
SLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAARASSSLGQGSGSQFVPTV
CNGREVVDSTTSSL

Sequence of entity 4 (C), FASTA

>8WGB_4 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains C)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMT
PELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVK
TTGIVETHFTFKDLYFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAA
AYIQCQFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY

Sequence of entity 5 (R), FASTA

>8WGB_5 Metabotropic glutamate receptor 4 (chains R)
KPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKPCGELKKEKGIHRLEAMLFALDRINND
PDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGGPPIITKPERVVG
VIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMVDI
VRALKWNYVSTVASEGSYGESGVEAFIQKSREDGGVCIAQSVKIPREPKAGEFDKIIRRL
LETSNARAVIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKIAPVLHLEEVAEGAV
TILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHVKKCTNRE
RIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRN
VNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLRIERMHWPGSG
QQLPRSICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTG
CRPIPIIKLEWGSPWAVLPLFLAVVGIAATLFVVITFVRYNDTPIVKASGRELSYVLLAG
IFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPR
FISPASQLAITFSLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLS
LICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSA
DKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKAVVTAATMSNK
FTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNHAI

Ligands and cofactors

ID	Name	Formula	Copies
W9R	(1R,2S)-2-[[3,5-bis(chloranyl)phenyl]carbamoyl]cyclohexane-1-carboxylic acid	C14 H15 Cl2 N O3	1
GLU	Glutamic acid	C5 H9 N O4	2

Primary citation

Structural basis of orientated asymmetry in a mGlu heterodimer. Huang, W., Jin, N., Guo, J. et al. Nat Commun (2024) 15:10345-10345. DOI 10.1038/s41467-024-54744-7 · PubMed

Other PDB entries of the same protein (UniProt P62873 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8QEH 1.43 Å, Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
8QEG 1.7 Å, Crystal structure of the G11 protein heterotrimer bound to YM-254890 inhibitor
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
9YDQ 1.94 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE and allosteric…
9YDP 1.95 Å, Human delta opioid receptor complex with mini-Gi and agonist DADLE
6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex

Browse structure collections

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