8WZ2: G-alpha q
Structure of 26RFa-pyroglutamylated RFamide peptide receptor complex. Determined by electron microscopy at 2.73 Å resolution. Released 19 Jun 2024.
- Method
- Electron microscopy
- Resolution
- 2.73 Å
- Organisms
- Homo sapiens, Rattus norvegicus, Bos taurus
- Chains
- 6
- Atoms
- 9,355
- Mol. weight
- 165.71 kDa
- Released
- 19 Jun 2024
Explore 8WZ2 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8WZ2 contains 40 α-helices and 60 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-30 | 24 | |
| β-strand | 34-40 | 7 | 1 |
| α-helix | 46-52 | 7 | |
| β-strand | 184-191 | 8 | 1 |
| β-strand | 194-201 | 8 | 1 |
| α-helix | 211-215 | 5 | |
| β-strand | 220-226 | 7 | 1 |
| α-helix | 233-244 | 12 | |
| β-strand | 253-259 | 7 | 1 |
| α-helix | 261-270 | 10 | |
| α-helix | 275-278 | 4 | |
| α-helix | 280-282 | 3 | |
| α-helix | 293-294 | 2 | |
| α-helix | 299-318 | 20 | |
| β-strand | 326-330 | 5 | 1 |
| α-helix | 338-357 | 20 | |
Chain B: 4 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-29 | 21 | |
| α-helix | 35-38 | 4 | |
| α-helix | 39-41 | 3 | |
| α-helix | 43-44 | 2 | |
| β-strand | 52-56 | 5 | 2 |
| β-strand | 63-68 | 6 | 3 |
| β-strand | 74-79 | 6 | 3 |
| β-strand | 83-88 | 6 | 3 |
| β-strand | 94-99 | 6 | 3 |
| β-strand | 105-110 | 6 | 4 |
| β-strand | 116-121 | 6 | 4 |
| β-strand | 125-130 | 6 | 4 |
| β-strand | 139-145 | 7 | 4 |
| β-strand | 151-156 | 6 | 5 |
| β-strand | 161-166 | 6 | 5 |
| β-strand | 171-175 | 5 | 5 |
| β-strand | 180-185 | 6 | 5 |
| β-strand | 192-197 | 6 | 6 |
| β-strand | 203-208 | 6 | 6 |
| β-strand | 213-217 | 5 | 6 |
| β-strand | 226-227 | 2 | 6 |
| β-strand | 234-239 | 6 | 7 |
| β-strand | 245-250 | 6 | 7 |
| β-strand | 255-259 | 5 | 7 |
| β-strand | 265-269 | 5 | 7 |
| β-strand | 278-283 | 6 | 8 |
| β-strand | 289-294 | 6 | 8 |
| β-strand | 298-303 | 6 | 8 |
| β-strand | 309-313 | 5 | 8 |
| β-strand | 320-325 | 6 | 2 |
| β-strand | 332-336 | 5 | 2 |
| β-strand | 341-344 | 4 | 2 |
Chain E: 3 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 2-6 | 5 | 9 |
| β-strand | 9-11 | 3 | 10 |
| β-strand | 16-24 | 9 | 9 |
| β-strand | 32-38 | 7 | 10 |
| β-strand | 44-50 | 7 | 10 |
| α-helix | 52-54 | 3 | |
| β-strand | 57-59 | 3 | 10 |
| β-strand | 68-72 | 5 | 9 |
| β-strand | 77-83 | 7 | 9 |
| β-strand | 91-98 | 8 | 10 |
| β-strand | 110 | 1 | 10 |
| β-strand | 114-118 | 5 | 10 |
| β-strand | 140-141 | 2 | 11 |
| β-strand | 146 | 1 | 12 |
| β-strand | 155-161 | 7 | 11 |
| β-strand | 166 | 1 | 13 |
| β-strand | 172 | 1 | 13 |
| β-strand | 174-179 | 6 | 12 |
| β-strand | 185-189 | 5 | 12 |
| β-strand | 195 | 1 | 12 |
| α-helix | 196 | 1 | |
| β-strand | 203-207 | 5 | 11 |
| β-strand | 211-216 | 6 | 11 |
| α-helix | 221-223 | 3 | |
| β-strand | 225-231 | 7 | 12 |
| β-strand | 238-239 | 2 | 12 |
| β-strand | 243-245 | 3 | 12 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-22 | 17 | |
| α-helix | 29-42 | 14 | |
| α-helix | 44-46 | 3 | |
| α-helix | 52-54 | 3 | |
Chain L: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 99-110 | 12 | |
Chain R: 18 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-18 | 9 | |
| α-helix | 23-29 | 7 | |
| α-helix | 39-41 | 3 | |
| α-helix | 42-71 | 30 | |
| α-helix | 75-77 | 3 | |
| α-helix | 79-107 | 29 | |
| α-helix | 117-148 | 32 | |
| α-helix | 150-156 | 7 | |
| α-helix | 159-183 | 25 | |
| β-strand | 184-191 | 8 | 14 |
| β-strand | 196-203 | 8 | 14 |
| α-helix | 208-219 | 12 | |
| α-helix | 220-224 | 5 | |
| α-helix | 225-242 | 18 | |
| α-helix | 262-297 | 36 | |
| α-helix | 301-304 | 4 | |
| α-helix | 307-322 | 16 | |
| α-helix | 324-329 | 6 | |
| α-helix | 330-334 | 5 | |
| α-helix | 337-343 | 7 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| G-alpha q | A | protein | 361 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 340 | Rattus norvegicus | P54311 (AlphaFold model) |
| scFV16 | E | protein | 247 | Homo sapiens | |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Bos taurus | P63212 (AlphaFold model) |
| Orexigenic neuropeptide QRFP | L | protein | 27 | Homo sapiens | Q8CE23 (AlphaFold model) |
| Pyroglutamylated RF-amide peptide receptor | R | protein | 431 | Homo sapiens | Q96P65 (AlphaFold model) |
Sequence of entity 1 (A), FASTA
>8WZ2_1 G-alpha q (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNL
V
Sequence of entity 2 (B), FASTA
>8WZ2_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (E), FASTA
>8WZ2_3 scFV16 (chains E)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL
Sequence of entity 4 (G), FASTA
>8WZ2_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (L), FASTA
>8WZ2_5 Orexigenic neuropeptide QRFP (chains L)
ASGPLGTLAEELSSYSRRKGGFSFRFG
Sequence of entity 6 (R), FASTA
>8WZ2_6 Pyroglutamylated RF-amide peptide receptor (chains R)
MQALNITPEQFSRLLRDHNLTREQFIALYRLRPLVYTPELPGRAKLALVLTGVLIFALAL
FGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFFCIPVTMLQNISDNWLGGAFICKM
VPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQYTNRRAFTMLGVVWLVAVIVGSPM
WHVQQLEIKYDFLYEKEHICCLEEWTSPVHQKIYTTFILVILFLLPLMVMLILYSKIGYE
LWIKKRVGDGSVLRTIHGKEMSKIARKKKRAVIMMVTVVALFAVCWAPFHVVHMMIEYSN
FEKEYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVLSAVCYCIVNKTFSPAQ
RHGNSGITMMRKKAKFSLRENPVEETKGEAFSDGNIEVKLCEQTEEKKKLKRHLALFRSE
LAENSPLDSGH
Primary citation
Structural basis for recognition of 26RFa by the pyroglutamylated RFamide peptide receptor. Jin, S., Guo, S., Xu, Y. et al. Cell Discov (2024) 10:58-58. DOI 10.1038/s41421-024-00670-3 · PubMed
Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
- 9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
- 22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
- 7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
- 9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
- 8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
- 9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
- 8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
- 9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
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