8X16: Adenosine receptor A3AR

Cryo-EM structure of adenosine receptor A3AR bound to CF101. Determined by electron microscopy at 3.29 Å resolution. Released 24 Apr 2024.

Method: Electron microscopy
Resolution: 3.29 Å
Organisms: Homo sapiens, Bos taurus, Rattus norvegicus
Chains: 5
Atoms: 8,751
Mol. weight: 149.22 kDa
Ligands: Q8L
Released: 24 Apr 2024

Explore 8X16 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8X16 contains 36 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	9-32	24
β-strand	34-39	6	3
α-helix	44-52	9
β-strand	183-191	9	3
β-strand	194-203	10	3
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	3
α-helix	227-230	4
α-helix	242-254	13
β-strand	263-269	7	3
α-helix	271-278	8
α-helix	283-285	3
α-helix	296-309	14
β-strand	319	1	3
β-strand	322-323	2	3
α-helix	330-350	21

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-39	2
β-strand	47-51	5	4
β-strand	58-63	6	5
β-strand	69-74	6	5
β-strand	78-83	6	5
β-strand	89-94	6	5
β-strand	100-105	6	6
β-strand	111-116	6	6
β-strand	122-125	4	6
β-strand	135-137	3	6
α-helix	145-146	2
β-strand	148-151	4	7
β-strand	156-160	5	7
β-strand	166-170	5	7
β-strand	175-180	6	7
β-strand	187-192	6	8
β-strand	198-203	6	8
β-strand	208-212	5	8
β-strand	218-222	5	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	260-264	5	9
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	304-308	5	10
β-strand	315-320	6	4
β-strand	327-331	5	4
β-strand	336-339	4	4

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	13-23	11
α-helix	30-43	14

Chain H: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	10-12	3	12
β-strand	17-25	9	11
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	59-60	2	12
β-strand	68-73	6	11
β-strand	78-84	7	11
α-helix	88-90	3
β-strand	92-99	8	12
β-strand	111	1	12
β-strand	115-119	5	12
β-strand	140-142	3	13
β-strand	146-148	3	14
β-strand	156-161	6	13
β-strand	166	1	15
β-strand	172	1	15
β-strand	174-179	6	14
β-strand	186-190	5	14
β-strand	194-195	2	14
α-helix	196	1
β-strand	203-207	5	13
β-strand	211-216	6	13
α-helix	221-223	3
β-strand	226-231	6	14
β-strand	239	1	14
β-strand	243-246	4	14

Chain R: 17 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	10-39	30
α-helix	41-43	3
α-helix	48-63	16
α-helix	66-74	9
β-strand	77-78	2	1
α-helix	80-113	34
α-helix	115-117	3
α-helix	118-121	4
α-helix	124-142	19
α-helix	145-147	3
β-strand	150	1	2
β-strand	164-165	2	1
β-strand	172	1	2
α-helix	176-180	5
α-helix	182-185	4
α-helix	187-208	22
α-helix	223-254	32
α-helix	261-278	18
α-helix	279-283	5
α-helix	284-286	3
α-helix	289-298	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Adenosine receptor A3	R	protein	318	Homo sapiens	P0DMS8 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Bos taurus	P63097 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Bos taurus	P63212 (AlphaFold model)
scFv16	H	protein	247	Mus musculus

Sequence of entity 1 (R), FASTA

>8X16_1 Adenosine receptor A3 (chains R)
MPNNSTALSLANVTYITMEIFIGLCAIVGNVLVICVVKLNPSLQTTTFYFIVSLALADIA
VGVLVMPLAIVVSLGITIHFYSCLFMTCLLLIFTHASIMSLLAIAVDRYLRVKLTVRYKR
VTTHRRIWLALGLCWLVSFLVGLTPMFGWNMKLTSEYHRNVTFLSCQFVSVMRMDYMVYF
SFLTWIFIPLVVMCAIYLDIFYIIRNKLSLNLSNSKETGAFYGREFKTAKSLFLVLFLFA
LSWLPLSIINCIIYFNGEVPQLVLYMGILLSHANSMMNPIVYAYKIKKFKETYLLILKAC
VVCHPSDSLDTSIEKNSE

Sequence of entity 2 (A), FASTA

>8X16_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (B), FASTA

>8X16_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>8X16_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (H), FASTA

>8X16_5 scFv16 (chains H)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL

Ligands and cofactors

ID	Name	Formula	Copies
Q8L	Piclidenoson	C18 H19 I N6 O4	1

Primary citation

Cryo-EM structures of adenosine receptor A 3 AR bound to selective agonists. Cai, H., Guo, S., Xu, Y. et al. Nat Commun (2024) 15:3252-3252. DOI 10.1038/s41467-024-47207-6 · PubMed

Other PDB entries of the same protein (UniProt P0DMS8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8X17 3.19 Å, Cryo-EM structure of adenosine receptor A3AR bound to CF102
9EHS 3.2 Å, Structure of a human adenosine A3 receptor complex bound to the covalent antagonist…
9EBH 3.6 Å, Human adenosine A3 receptor Gi1 complex bound to adenosine
9EBI 3.6 Å, Human adenosine A3 receptor Gi complex (mini-Gsi chimera) bound to Piclidenoson (CF101,…

Browse structure collections

About this viewer

MolViewer shows 8X16 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.