9EBI: Human adenosine A3 receptor Gi complex

Human adenosine A3 receptor Gi complex (mini-Gsi chimera) bound to Piclidenoson (CF101, IB-MECA). Determined by electron microscopy at 3.6 Å resolution. Released 20 Aug 2025.

Method: Electron microscopy
Resolution: 3.6 Å
Organisms: Homo sapiens, Lama glama
Chains: 5
Atoms: 7,932
Mol. weight: 130.79 kDa
Ligands: Q8L
Released: 20 Aug 2025

Explore 9EBI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9EBI contains 41 α-helices and 51 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-40	8	2
α-helix	42-44	3
α-helix	46-48	3
β-strand	168-174	7	2
β-strand	177-183	7	2
α-helix	194-198	5
β-strand	203-209	7	2
α-helix	226-238	13
β-strand	246-252	7	2
α-helix	254-263	10
α-helix	268-271	4
α-helix	273-275	3
α-helix	286-287	2
α-helix	292-311	20
β-strand	319-323	5	2
α-helix	331-350	20

Chain B: 4 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
α-helix	186	1
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-308	6	9
β-strand	315	1	10
β-strand	319-320	2	3
β-strand	327-330	4	3
β-strand	331	1	10
β-strand	336-339	4	3

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	27-29	3
α-helix	30-43	14
α-helix	53-55	3

Chain N: 2 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	34-39	6	13
β-strand	45-51	7	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-94	3	14
β-strand	95-98	4	13
β-strand	118	1	13
β-strand	122-124	3	14
β-strand	125-126	2	12

Chain R: 20 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	10-39	30
α-helix	41-43	3
α-helix	47-60	14
α-helix	61-65	5
α-helix	66-74	9
β-strand	77-78	2	1
α-helix	80-113	34
α-helix	115-117	3
α-helix	118-121	4
α-helix	124-142	19
α-helix	145-147	3
α-helix	151-153	3
β-strand	164-165	2	1
α-helix	168-171	4
α-helix	174-175	2
α-helix	176-180	5
α-helix	187-206	20
α-helix	229-254	26
α-helix	261-273	13
α-helix	278	1
α-helix	279-283	5
α-helix	287-292	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Adenosine receptor A3	R	protein	363	Homo sapiens	P0DMS8 (AlphaFold model)
mini-Gs/i chimera	A	protein	257	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	349	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	70	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	128	Lama glama

Sequence of entity 1 (R), FASTA

>9EBI_1 Adenosine receptor A3 (chains R)
DYKDDDDAANFTPVNGSSGNQSVRLVTSSSLEVLFQGPPNNSTALSLANVTYITMEIFIG
LCAIVGNVLVICVVKLNPSLQTTTFYFIVSLALADIAVGVLVMPLAIVVSLGITIHFYSC
LFMTCLLLIFTHASIMSLLAIAVDRYLRVKLTVRYKRVTTHRRIWLALGLCWLVSFLVGL
TPMFGWNMKLTSEYHRNVTFLSCQFVSVMRMDYMVYFSFLTWIFIPLVVMCAIYLDIFYI
IRNKLSLNLSNSKETGAFYGREFKTAKSLFLVLFLFALSWLPLSIINCIIYFNGEVPQLV
LYMGILLSHANSMMNPIVYAYKIKKFKETYLLILKACVVCHPSDSLDTSIEKNSEHHHHH
HHH

Sequence of entity 2 (A), FASTA

>9EBI_2 mini-Gs/i chimera (chains A)
GGGSLEVLFQGPGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGADNSGKSTI
VKQMRILHGGSGGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFV
VDSSDYNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFAR
YTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENARRIFN
DVTDIIIKMNLRDCGLF

Sequence of entity 3 (B), FASTA

>9EBI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTL
RGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNY
VACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDI
ETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINA
ICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDD
FNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9EBI_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPF
REKKFFCAIL

Sequence of entity 5 (N), FASTA

>9EBI_5 Nb35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSS

Ligands and cofactors

ID	Name	Formula	Copies
Q8L	Piclidenoson	C18 H19 I N6 O4	1

Primary citation

Molecular basis of ligand binding and receptor activation at the human A 3 adenosine receptor. Zhang, L., Mobbs, J.I., Bennetts, F.M. et al. Nat Commun (2025) 16:7674-7674. DOI 10.1038/s41467-025-62872-x · PubMed

Other PDB entries of the same protein (UniProt P0DMS8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8X17 3.19 Å, Cryo-EM structure of adenosine receptor A3AR bound to CF102
9EHS 3.2 Å, Structure of a human adenosine A3 receptor complex bound to the covalent antagonist…
8X16 3.29 Å, Cryo-EM structure of adenosine receptor A3AR bound to CF101
9EBH 3.6 Å, Human adenosine A3 receptor Gi1 complex bound to adenosine

Browse structure collections

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