8X17: Adenosine receptor A3AR
Cryo-EM structure of adenosine receptor A3AR bound to CF102. Determined by electron microscopy at 3.19 Å resolution. Released 24 Apr 2024.
- Method
- Electron microscopy
- Resolution
- 3.19 Å
- Organisms
- Rattus norvegicus, Homo sapiens, Bos taurus
- Chains
- 5
- Atoms
- 8,753
- Mol. weight
- 149.26 kDa
- Ligands
- XS0
- Released
- 24 Apr 2024
Explore 8X17 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8X17 contains 38 α-helices and 59 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-32 | 24 | |
| β-strand | 34-39 | 6 | 14 |
| α-helix | 44-52 | 9 | |
| β-strand | 185-191 | 7 | 14 |
| β-strand | 194-200 | 7 | 14 |
| α-helix | 208-210 | 3 | |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 14 |
| α-helix | 227-230 | 4 | |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 14 |
| α-helix | 271-278 | 8 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-308 | 13 | |
| β-strand | 319-323 | 5 | 14 |
| α-helix | 330-351 | 22 | |
Chain B: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 30-34 | 5 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 1 |
| β-strand | 58-63 | 6 | 2 |
| β-strand | 69-74 | 6 | 2 |
| β-strand | 78-83 | 6 | 2 |
| β-strand | 88-94 | 7 | 2 |
| β-strand | 100-105 | 6 | 3 |
| β-strand | 111-116 | 6 | 3 |
| β-strand | 122-125 | 4 | 3 |
| β-strand | 134-137 | 4 | 3 |
| β-strand | 146-153 | 8 | 4 |
| β-strand | 156-161 | 6 | 4 |
| β-strand | 166-170 | 5 | 4 |
| β-strand | 175-180 | 6 | 4 |
| β-strand | 187-192 | 6 | 5 |
| β-strand | 198-203 | 6 | 5 |
| β-strand | 208-212 | 5 | 5 |
| β-strand | 221-222 | 2 | 5 |
| β-strand | 229-234 | 6 | 6 |
| β-strand | 240-245 | 6 | 6 |
| β-strand | 250-254 | 5 | 6 |
| β-strand | 259-264 | 6 | 6 |
| β-strand | 273-278 | 6 | 7 |
| β-strand | 284-289 | 6 | 7 |
| β-strand | 294-298 | 5 | 7 |
| β-strand | 304-307 | 4 | 7 |
| β-strand | 315-320 | 6 | 1 |
| β-strand | 327-331 | 5 | 1 |
| β-strand | 336-339 | 4 | 1 |
Chain C: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-23 | 11 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
Chain H: 3 helices, 23 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 10-12 | 3 | 10 |
| β-strand | 17-25 | 9 | 9 |
| β-strand | 33-39 | 7 | 10 |
| β-strand | 45-51 | 7 | 10 |
| β-strand | 59-60 | 2 | 10 |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-84 | 7 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 10 |
| β-strand | 111 | 1 | 10 |
| β-strand | 115-119 | 5 | 10 |
| β-strand | 141-142 | 2 | 11 |
| β-strand | 146-148 | 3 | 12 |
| β-strand | 155-160 | 6 | 11 |
| β-strand | 166 | 1 | 13 |
| β-strand | 172 | 1 | 13 |
| β-strand | 174-179 | 6 | 12 |
| β-strand | 185-190 | 6 | 12 |
| β-strand | 194-195 | 2 | 12 |
| α-helix | 196 | 1 | |
| β-strand | 203-207 | 5 | 11 |
| β-strand | 211-216 | 6 | 11 |
| α-helix | 221-223 | 3 | |
| β-strand | 226-231 | 6 | 12 |
| β-strand | 243-246 | 4 | 12 |
Chain R: 19 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-39 | 32 | |
| α-helix | 41-43 | 3 | |
| α-helix | 46-60 | 15 | |
| α-helix | 61-65 | 5 | |
| α-helix | 66-74 | 9 | |
| β-strand | 79 | 1 | 8 |
| α-helix | 80-113 | 34 | |
| α-helix | 118-121 | 4 | |
| α-helix | 124-142 | 19 | |
| α-helix | 151-153 | 3 | |
| α-helix | 158-160 | 3 | |
| β-strand | 163 | 1 | 8 |
| α-helix | 168-171 | 4 | |
| α-helix | 174-176 | 3 | |
| α-helix | 177-187 | 11 | |
| α-helix | 188-208 | 21 | |
| α-helix | 224-252 | 29 | |
| α-helix | 261-272 | 12 | |
| α-helix | 274-278 | 5 | |
| α-helix | 279-283 | 5 | |
| α-helix | 287-299 | 13 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 345 | Rattus norvegicus | P54311 (AlphaFold model) |
| Adenosine receptor A3 | R | protein | 318 | Homo sapiens | P0DMS8 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | C | protein | 71 | Bos taurus | P63212 (AlphaFold model) |
| scFv16 | H | protein | 247 | Mus musculus | |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | A | protein | 354 | Bos taurus | P63097 (AlphaFold model) |
Sequence of entity 1 (B), FASTA
>8X17_1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 2 (R), FASTA
>8X17_2 Adenosine receptor A3 (chains R)
MPNNSTALSLANVTYITMEIFIGLCAIVGNVLVICVVKLNPSLQTTTFYFIVSLALADIA
VGVLVMPLAIVVSLGITIHFYSCLFMTCLLLIFTHASIMSLLAIAVDRYLRVKLTVRYKR
VTTHRRIWLALGLCWLVSFLVGLTPMFGWNMKLTSEYHRNVTFLSCQFVSVMRMDYMVYF
SFLTWIFIPLVVMCAIYLDIFYIIRNKLSLNLSNSKETGAFYGREFKTAKSLFLVLFLFA
LSWLPLSIINCIIYFNGEVPQLVLYMGILLSHANSMMNPIVYAYKIKKFKETYLLILKAC
VVCHPSDSLDTSIEKNSE
Sequence of entity 3 (C), FASTA
>8X17_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (H), FASTA
>8X17_4 scFv16 (chains H)
VQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYA
DTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSA
GGGGSGGGGSGGGGSADIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLEL
Sequence of entity 5 (A), FASTA
>8X17_5 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| XS0 | namodenoson | C18 H18 Cl I N6 O4 | 1 |
Primary citation
Cryo-EM structures of adenosine receptor A 3 AR bound to selective agonists. Cai, H., Guo, S., Xu, Y. et al. Nat Commun (2024) 15:3252-3252. DOI 10.1038/s41467-024-47207-6 · PubMed
Other PDB entries of the same protein (UniProt P54311 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
- 9EJZ 2.06 Å, Human M5 muscarinic acetylcholine receptor complex with mini-Gq, agonist acetylcholine…
- 9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 8Z9O 2.4 Å, Cryo-EM structure of human GPR4-Gs complex
- 22ES 2.43 Å, Gi bound kappa-opioid receptor in complex with difelikefalin
- 7MBY 2.44 Å, Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
- 9WG6 2.44 Å, D1R-Gs in complexed with Dopamine/LY3154207/BMSA1/UNC9815
- 8JIS 2.46 Å, Cryo-EM structure of the GLP-1R/GCGR dual agonist peptide15-bound human GLP-1R-Gs complex
- 9M0R 2.47 Å, Structure of neuropeptide FF receptor 1 complex with NPVF
- 8YKV 2.48 Å, Cryo-EM structure of succinate receptor SUCR1 bound to compound 31
- 9M1O 2.49 Å, Cryo-EM structures of NPFFR2 complex with neuropeptide FF
Browse structure collections
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