8XIR: Pasireotide-SSTR3 G protein complex

Structure of pasireotide-SSTR3 G protein complex. Determined by electron microscopy at 2.52 Å resolution. Released 3 Jul 2024.

Method: Electron microscopy
Resolution: 2.52 Å
Organisms: Homo sapiens, Lama glama, synthetic construct
Chains: 6
Atoms: 8,190
Mol. weight: 154.5 kDa
Released: 3 Jul 2024

Explore 8XIR in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XIR contains 34 α-helices and 49 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-67	27
α-helix	68-72	5
α-helix	78-106	29
α-helix	113-146	34
α-helix	148-154	7
α-helix	157-180	24
β-strand	182	1	1
β-strand	193	1	1
α-helix	203-211	9
α-helix	212-216	5
α-helix	217-238	22
α-helix	242-244	3
α-helix	246-249	4
α-helix	251-276	26
α-helix	278-281	4
α-helix	289-310	22
α-helix	311-315	5
α-helix	321-327	7

Chain B: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-32	26
β-strand	34-40	7	2
α-helix	46-50	5
β-strand	184-191	8	2
β-strand	194-201	8	2
α-helix	211-215	5
β-strand	220-226	7	2
α-helix	233-245	13
β-strand	253-259	7	2
α-helix	261-270	10
α-helix	280-284	5
α-helix	293-294	2
α-helix	299-318	20
β-strand	326-329	4	2
α-helix	338-358	21

Chain C: 4 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	14-30	17
α-helix	35-38	4
α-helix	43-44	2
β-strand	52-57	6	3
β-strand	63-68	6	4
β-strand	74-79	6	4
β-strand	83-88	6	4
β-strand	94-99	6	4
α-helix	100	1
β-strand	105-110	6	5
β-strand	116-121	6	5
β-strand	125	1	6
β-strand	127-130	4	5
β-strand	139-142	4	5
β-strand	145	1	6
β-strand	151-153	3	7
β-strand	156	1	7
β-strand	161-166	6	7
β-strand	171-175	5	7
β-strand	181-185	5	7
β-strand	192-197	6	8
β-strand	203-208	6	8
β-strand	212-217	6	8
β-strand	223-228	6	8
β-strand	234-239	6	9
β-strand	245-250	6	9
β-strand	254-259	6	9
β-strand	264-270	7	9
β-strand	278-283	6	10
β-strand	289-294	6	10
β-strand	299-303	5	10
β-strand	309-313	5	10
β-strand	320-325	6	3
β-strand	332-336	5	3
β-strand	340-344	5	3

Chain D: 2 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	5-9	5	11
β-strand	20-27	8	11
α-helix	31-33	3
β-strand	36-41	6	12
β-strand	47-53	7	12
β-strand	60-62	3	12
β-strand	70-75	6	11
β-strand	80-85	6	11
α-helix	90-92	3
β-strand	94-100	7	12
β-strand	120	1	12
β-strand	124-126	3	12

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-22	13
α-helix	30-43	14
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 3	A	protein	418	Homo sapiens	P32745 (AlphaFold model)
G-alpha i	B	protein	361	Homo sapiens
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	377	Homo sapiens	P62873 (AlphaFold model)
nanobody Nb35	D	protein	156	Lama glama
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Pasireotide	F	protein	6	synthetic construct

Sequence of entity 1 (A), FASTA

>8XIR_1 Somatostatin receptor type 3 (chains A)
MDMLHPSSVSTTSEPENASSAWPPDATLGNVSAGPSPAGLAVSGVLIPLVYLVVCVVGLL
GNSLVIYVVLRHTASPSVTNVYILNLALADELFMLGLPFLAAQNALSYWPFGSLMCRLVM
AVDGINQFTSIFCLTVMSVDRYLAVVHPTRSARWRTAPVARTVSAAVWVASAVVVLPVVV
FSGVPRGMSTCHMQWPEPAAAWRAGFIIYTAALGFFGPLLVICLCYLLIVVKVRSAGRRV
WAPSCQRRRRSERRVTRMVVAYVALFVLCWMPFYVLNIVNVVCPLPEEPAFFGLYFLVVA
LPYANSCANPILYGFLSYRFKQGFRRVLLRPSRRVRSQEPTVGPPEKTEEEDEEEEDGEE
SREGGKGKEMNGRVSQITQPGTSGQERPPSRVASKEQQLLPQEASTGEKSSTMRISYL

Sequence of entity 2 (B), FASTA

>8XIR_2 G-alpha i (chains B)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDVTDIIIKMNLRDCGL
F

Sequence of entity 3 (C), FASTA

>8XIR_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSG
GGGSSGVSGWRLFKKIS

Sequence of entity 4 (D), FASTA

>8XIR_4 nanobody Nb35 (chains D)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHH

Sequence of entity 5 (G), FASTA

>8XIR_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (F), FASTA

>8XIR_6 Pasireotide (chains F)
XWKYFX

Primary citation

Selective ligand recognition and activation of somatostatin receptors SSTR1 and SSTR3. Wang, Y., Xu, Y., Wang, Y. et al. Proc Natl Acad Sci U S A (2024) 121:e2400298121-e2400298121. DOI 10.1073/pnas.2400298121 · PubMed

Other PDB entries of the same protein (UniProt P32745 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8XIQ 2.71 Å, Structure of L796778-SSTR3 G protein complex
8ZBI 2.79 Å, Cryo-EM structure of Somatostatin receptor 3 (SSTR3) with Gi1 complex

Browse structure collections

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