8YLE: Werner syndrome helicase

Crystal structure of Werner syndrome helicase complexed with AMP-PCP. Determined by X-ray diffraction at 1.86 Å resolution. Released 10 Apr 2024.

Method: X-ray diffraction
Resolution: 1.86 Å
Organism: Homo sapiens
Chains: 1
Atoms: 3,595
Mol. weight: 49.76 kDa
Ligands: ACP, ZN
Released: 10 Apr 2024

Explore 8YLE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8YLE contains 26 α-helices and 16 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 26 helices, 16 β-strands

Element	Residues	Length	Sheet
α-helix	13-15	3
α-helix	17-22	6
α-helix	23-27	5
α-helix	34-44	11
β-strand	50-53	4	1
α-helix	60-71	12
β-strand	74-78	5	1
α-helix	82-94	13
β-strand	99-101	3	1
α-helix	108-114	7
β-strand	121-124	4	1
α-helix	126-130	5
α-helix	133-142	10
β-strand	145-150	6	1
α-helix	153-156	4
α-helix	165-168	4
α-helix	169-172	4
α-helix	173-176	4
β-strand	182-186	5	1
α-helix	191-201	11
β-strand	207-210	4	1
β-strand	218-224	7	2
α-helix	229-233	5
α-helix	234-236	3
β-strand	237-239	3	3
β-strand	244-246	3	3
β-strand	250-253	4	2
α-helix	257-269	13
β-strand	274-277	4	2
α-helix	283-294	12
β-strand	300-303	4	2
α-helix	309-311	3
β-strand	318-322	5	2
α-helix	328-335	8
β-strand	345-351	7	2
α-helix	353-364	12
α-helix	369-387	19
α-helix	392-401	10
α-helix	404-406	3
α-helix	407-409	3
β-strand	419	1	2
α-helix	420-427	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN	A	protein	430	Homo sapiens	Q14191 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8YLE_1 Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (chains A)
NEGEEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYG
KSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKY
RIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRDSFRKLGSLKTALPM
VPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVK
TSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQ
CVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLN
RHLLTEIRNEKFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCC
DNCRSRLDHA

Ligands and cofactors

ID	Name	Formula	Copies
ACP	Phosphomethylphosphonic acid adenylate ester	C11 H18 N5 O12 P3	1
ZN	Zinc ion	Zn	2

Water and common crystallization additives (EDO) are not listed.

Primary citation

Structure of werner syndrome helicase complexed with AMP-PCP at 1.86 Angstroms resolution. Chen, R. To be published.

Other PDB entries of the same protein (UniProt Q14191 (AlphaFold model), which also has an AlphaFold model), best resolution first:

10AK 1.37 Å, Crystal Structure of Human WRN helicase with compound 4
7GQU 1.54 Å, Crystal Structure of Werner helicase fragment 517-945 in covalent complex with…
9OWB 1.56 Å, Human WRN helicase in complex with allosteric ligand Compound 6
7GQS 1.57 Å, Crystal Structure of Werner helicase fragment 517-945 in complex with ADP
9MJU 1.58 Å, Crystal structure of WRN helicase with allosteric fragment 1
7XUT 1.6 Å, Crystal structure of RPA70N-WRN fusion
8PFP 1.6 Å, Crystal structure of WRN helicase domain in complex with ATPgammaS
9MJY 1.63 Å, Crystal structure of WRN helicase with bound allosteric compound 1
9OG8 1.66 Å, Crystal structure of WRN in complex with compound 43
9OWC 1.67 Å, Human WRN helicase in complex with allosteric ligand Compound 7
9MJZ 1.7 Å, Crystal structure of WRN helicase with bound allosteric compound 3
9MJT 1.73 Å, Crystal structure of apo WRN helicase in Form D

Browse structure collections

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