8ZAB: Ectodomains of HBMBPP-BTN2A1-BTN3A1 complex

Cryo-EM structure of ectodomains of HBMBPP-BTN2A1-BTN3A1 complex. Determined by electron microscopy at 3.67 Å resolution. Released 30 Apr 2025.

Method: Electron microscopy
Resolution: 3.67 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,497
Mol. weight: 93.87 kDa
Released: 30 Apr 2025

Explore 8ZAB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZAB contains 19 α-helices and 100 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 5 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	1
β-strand	11-14	4	2
β-strand	15	1	3
β-strand	17	1	3
β-strand	25	1	1
β-strand	36-40	5	2
β-strand	48-51	4	2
β-strand	54-55	2	2
α-helix	57-59	3
β-strand	70	1	4
α-helix	72-77	6
β-strand	82	1	4
β-strand	93-98	6	2
β-strand	100-101	2	5
β-strand	104-105	2	5
β-strand	108-115	8	2
β-strand	116	1	6
β-strand	122-129	8	7
β-strand	132-138	7	7
β-strand	139-142	4	8
β-strand	143	1	6
α-helix	145-146	2
β-strand	147-151	5	9
β-strand	157	1	9
β-strand	161-162	2	7
α-helix	163-165	3
β-strand	166-167	2	8
β-strand	173-175	3	8
β-strand	177-181	5	7
β-strand	189-195	7	9
β-strand	200-208	9	9
α-helix	210-212	3

Chain D: 3 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	10
β-strand	11-14	4	11
β-strand	19-25	7	10
β-strand	36-40	5	11
β-strand	46-50	5	11
β-strand	55	1	11
β-strand	68-71	4	10
β-strand	79-84	6	10
β-strand	93-99	7	11
β-strand	101	1	12
β-strand	104	1	12
β-strand	109-115	7	11
β-strand	116	1	13
β-strand	122-128	7	9
β-strand	133-142	10	9
β-strand	143	1	13
β-strand	147-151	5	14
β-strand	161-162	2	9
α-helix	163-165	3
β-strand	166-167	2	9
α-helix	172	1
β-strand	173-178	6	9
β-strand	180	1	9
β-strand	190-194	5	14
β-strand	201-202	2	14
β-strand	205-208	4	7
α-helix	210-212	3

Chain E: 6 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	3-5	3	15
β-strand	11-14	4	16
β-strand	19-23	5	17
β-strand	24-26	3	15
β-strand	35-39	5	16
β-strand	46	1	18
β-strand	48-51	4	16
β-strand	54-55	2	16
β-strand	60	1	18
α-helix	62-64	3
β-strand	68-71	4	17
α-helix	75-77	3
β-strand	80-84	5	17
α-helix	89-91	3
β-strand	93-101	9	16
β-strand	104-115	12	16
β-strand	116	1	19
α-helix	119-121	3
β-strand	122-128	7	20
β-strand	133-142	10	20
β-strand	143	1	19
β-strand	147-151	5	21
α-helix	158-160	3
β-strand	162-167	6	20
β-strand	173-181	9	20
β-strand	190-195	6	21
β-strand	200-202	3	21
β-strand	205	1	21
α-helix	210-212	3

Chain G: 5 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	11-14	4	22
β-strand	21-23	3	23
β-strand	34-39	6	24
β-strand	46	1	25
β-strand	48-52	5	24
α-helix	57-59	3
β-strand	60	1	25
α-helix	62-64	3
β-strand	70-71	2	23
β-strand	80-82	3	23
α-helix	89-91	3
β-strand	95	1	26
β-strand	96-100	5	24
β-strand	105-107	3	24
β-strand	110	1	26
β-strand	112-115	4	22
β-strand	116	1	27
α-helix	117-121	5
β-strand	122-129	8	28
β-strand	132-138	7	28
β-strand	142	1	29
β-strand	143	1	27
β-strand	147-151	5	30
β-strand	157	1	30
β-strand	162-164	3	28
β-strand	167	1	29
β-strand	173	1	29
β-strand	176-181	6	28
β-strand	188-194	7	30
β-strand	201-207	7	30
α-helix	210-212	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Butyrophilin subfamily 3 member A1	C, D	protein	213	Homo sapiens	O00481 (AlphaFold model)
Butyrophilin subfamily 2 member A1	E, G	protein	214	Homo sapiens	Q7KYR7 (AlphaFold model)

Sequence of entity 1 (C, D), FASTA

>8ZAB_1 Butyrophilin subfamily 3 member A1 (chains C, D)
QFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQ
SAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSD
LHVDVKGYKDGGIHLECRSTGWYPQPQIQWSNNKGENIPTVEAPVVADGVGLYAVAASVI
MRGSSGEGVSCTIRSSLLGLEKTASISIADPFF

Sequence of entity 2 (E, G), FASTA

>8ZAB_2 Butyrophilin subfamily 2 member A1 (chains E, G)
QFIVVGPTDPILATVGENTTLRCHLSPEKNAEDMEVRWFRSQFSPAVFVYKGGRERTEEQ
MEEYRGRTTFVSKDISRGSVALVIHNITAQENGTYRCYFQEGRSYDEAILHLVVAGLGSK
PLISMRGHEDGGIRLECISRGWYPKPLTVWRDPYGGVAPALKEVSMPDADGLFMVTTAVI
IRDKSVRNMSCSINNTLLGQKKESVIFIPESFMP

Primary citation

Phosphoantigen-induced inside-out stabilization of butyrophilin receptor complexes drives dimerization-dependent gamma delta TCR activation. Zhu, Y., Gao, W., Zheng, J. et al. Immunity (2025) 58:1646-1659.e5. DOI 10.1016/j.immuni.2025.04.012 · PubMed

Other PDB entries of the same protein (UniProt O00481 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6ITA 1.2 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 mutant
6ISM 1.25 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 mutant
8IZE 1.4 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 in complex with 4-HMBPP
4N7I 1.4 Å, Crystal Structure of Intracellular B30.2 Domain of BTN3A1
4N7U 1.46 Å, Crystal Structure of Intracellular B30.2 Domain of BTN3A1 in Complex with CHDMAPP
5LYG 1.6 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 bound to malonate
8IZG 1.6 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 in complex with 5-HMBPP
5LYK 1.7 Å, Crystal structure of intracellular B30.2 domain of BTN3A1 bound to citrate
8IXV 1.72 Å, Crystal structure of intracellular B30.2 domain of BTN3A in complex with 2Cl-HMBPP
4K55 1.91 Å, Structure of the extracellular domain of butyrophilin BTN3A1 in complex with…
5HM7 1.93 Å, The Intracellular domain of Butyrophilin 3A1 protein
4F80 1.94 Å, Crystal Structure of the human BTN3A1 ectodomain

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