8ZSV: RO5263397-bound mTAAR1-Gs complex

Cryo-EM structure of the RO5263397-bound mTAAR1-Gs complex. Determined by electron microscopy at 2.96 Å resolution. Released 24 Jul 2024.

Method: Electron microscopy
Resolution: 2.96 Å
Organisms: Homo sapiens, Lama glama, Escherichia coli
Chains: 5
Atoms: 8,070
Mol. weight: 144.77 kDa
Ligands: A1D8X
Released: 24 Jul 2024

Explore 8ZSV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8ZSV contains 38 α-helices and 51 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-36	24
β-strand	40-46	7	1
β-strand	72-75	4	1
β-strand	80-84	5	1
α-helix	96-99	4
β-strand	105-108	4	1
β-strand	109-111	3	2
α-helix	118-129	12
α-helix	134-136	3
β-strand	138-139	2	1
β-strand	142-144	3	2
α-helix	146-155	10
α-helix	160-163	4
α-helix	184-203	20
β-strand	214-215	2	2
α-helix	223-242	20

Chain B: 6 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-52	6	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
α-helix	95	1
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187	1	7
β-strand	191-192	2	8
β-strand	198-202	5	8
β-strand	203	1	7
β-strand	207-212	6	8
β-strand	218-223	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
α-helix	272	1
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	294-298	5	10
β-strand	303-308	6	10
β-strand	319-320	2	3
β-strand	327-330	4	3
β-strand	335-339	5	3

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	7-23	17
α-helix	30-43	14
α-helix	53-55	3

Chain N: 3 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	19-25	7	11
α-helix	29-31	3
β-strand	34	1	13
β-strand	35-39	5	14
β-strand	45-50	6	14
β-strand	59-60	2	14
α-helix	62-64	3
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-95	4	14
β-strand	98	1	13
β-strand	122-124	3	14
β-strand	125-126	2	12

Chain R: 18 helices, 0 β-strands

Element	Residues	Length
α-helix	21-49	29
α-helix	57-70	14
α-helix	71-75	5
α-helix	76-85	10
α-helix	92-125	34
α-helix	127-129	3
α-helix	130-133	4
α-helix	136-157	22
α-helix	168-171	4
α-helix	172-176	5
α-helix	187-194	8
α-helix	195-199	5
α-helix	200-226	27
α-helix	246-250	5
α-helix	252-274	23
α-helix	280-298	19
α-helix	299-303	5
α-helix	306-312	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	247	Homo sapiens	P04899 (AlphaFold model), P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nb35	N	protein	139	Lama glama
Soluble cytochrome b562,Trace amine-associated receptor 1	R	protein	488	Escherichia coli, Mus musculus	P0ABE7, Q923Y8

Sequence of entity 1 (A), FASTA

>8ZSV_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKAAAATHRLLLLGADNSGKSTIVKQMRIYHGG
SGGSGVNSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQ
EALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPE
PGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMH
LRQYELL

Sequence of entity 2 (B), FASTA

>8ZSV_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>8ZSV_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>8ZSV_4 Nb35 (chains N)
MQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASIS
YTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRG
QGTQVTVSSHHHHHHEPEA

Sequence of entity 5 (R), FASTA

>8ZSV_5 Soluble cytochrome b562,Trace amine-associated receptor 1 (chains R)
MKTIIALSYIFCLVFADYKDDDDKHHHHHHHHHHLEVLFQGPADLEDNWETLNDNLKVIE
KADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKL
ANEGKVKEAQAAAEQLKTTRNAYIQKYLMGQPGNGSAHLCHAITNISHRNSDWSREVQAS
LYSLMSLIILATLVGNLIVIISISHFKQLHTPTNWLLHSMAIVDFLLGCLIMPCSMVRTV
ERCWYFGEILCKVHTSTDIMLSSASIFHLAFISIDRYCAVCDPLRYKAKINISTILVMIL
VSWSLPAVYAFGMIFLELNLKGVEELYRSQVSDLGGCSPFFSKVSGVLAFMTSFYIPGSV
MLFVYYRIYFIAKGQARSINRTNVQVGLEGKSQAPQSKETKAAKTLGIMVGVFLVCWCPF
FLCTVLDPFLGYVIPPSLNDALYWFGYLNSALNPMVYAFFYPWFRRALKMVLLGKIFQKD
SSRSKLFL

Ligands and cofactors

ID	Name	Formula	Copies
A1D8X	(4~{S})-4-(3-fluoranyl-2-methyl-phenyl)-1,3-oxazolidin-2-amine	C10 H11 F N2 O	1

Primary citation

The versatile binding landscape of the TAAR1 pocket for LSD and other antipsychotic drug molecules. Jiang, K., Zheng, Y., Zeng, L. et al. Cell Rep (2024) 43:114505-114505. DOI 10.1016/j.celrep.2024.114505 · PubMed

Other PDB entries of the same protein (UniProt P04899 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8ZBW 2.58 Å, Cryo-EM structure of formyl peptide receptor 2/C1R receptor in complex with Gi
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
7YK7 2.75 Å, Cryo-EM structure of the DC591053-bound human relaxin family peptide receptor 4…
9K6L 2.77 Å, Cryo-EM structure of GPCR16-Gi2 complex
9XDQ 2.79 Å, Cryo-EM structure of BMS986187 bound DOR-Gi complex
7WVX 2.8 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fhumanin and Gi2
8W8A 2.8 Å, Cryo-EM structure of the RO5256390-TAAR1 complex
8ZSJ 2.8 Å, Cryo-EM structure of the apo hTAAR1-Gs complex
7WVV 2.9 Å, Cryo-EM structure of the human formyl peptide receptor 2 in complex with fMLFII and Gi2
9DQJ 2.9 Å, CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
9KFI 2.91 Å, Cryo-EM structure of the human relaxin family peptide receptor 3 in complex with…
9KFK 2.95 Å, Cryo-EM structure of the relaxin-3-bound human relaxin family peptide receptor 4…

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