9AUC: Human Amylin1 Receptor

Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide. Determined by electron microscopy at 2.4 Å resolution. Released 24 Apr 2024.

Method: Electron microscopy
Resolution: 2.4 Å
Organisms: Homo sapiens, Lama glama
Chains: 7
Atoms: 10,369
Mol. weight: 189.47 kDa
Ligands: PLM, NAG, Y01
Released: 24 Apr 2024

Explore 9AUC in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9AUC contains 48 α-helices and 59 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-39	28
β-strand	41-46	6	1
α-helix	53-59	7
β-strand	207-213	7	1
β-strand	218-224	7	1
α-helix	234-238	5
β-strand	243-249	7	1
α-helix	265-277	13
β-strand	286-292	7	1
α-helix	294-302	9
α-helix	313-315	3
α-helix	332-349	18
β-strand	359-363	5	1
α-helix	370-390	21

Chain B: 2 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain E: 9 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	29-35	7
α-helix	41-51	11
α-helix	55-57	3
α-helix	62-80	19
α-helix	87-99	13
α-helix	104-106	3
β-strand	111	1	11
α-helix	113-115	3
α-helix	116-119	4
α-helix	121-142	22

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	9-21	13
α-helix	30-42	13
α-helix	53-55	3

Chain N: 1 helix, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-12	3	10
β-strand	18-25	8	9
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	68-73	6	9
β-strand	78-83	6	9
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-126	5	10

Chain P: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	5-7	3
α-helix	8-18	11
α-helix	28-30	3

Chain R: 22 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	43-61	19
β-strand	71-72	2	12
α-helix	73-74	2
β-strand	76	1	13
β-strand	81	1	13
β-strand	85-86	2	12
β-strand	93-94	2	14
α-helix	95-96	2
β-strand	107-108	2	14
β-strand	109	1	15
β-strand	112	1	16
β-strand	118	1	16
β-strand	120	1	17
β-strand	127	1	17
β-strand	130	1	15
α-helix	133-135	3
α-helix	138-172	35
α-helix	179-200	22
α-helix	201-205	5
α-helix	209-214	6
α-helix	218-245	28
α-helix	246-250	5
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-281	11
β-strand	285	1	11
α-helix	288-290	3
α-helix	296-300	5
α-helix	302-329	28
α-helix	334-352	19
α-helix	355-358	4
α-helix	366-381	16
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-405	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768 (AlphaFold model)
Nanobody 35	N	protein	138	Lama glama
Receptor activity-modifying protein 1	E	protein	149	Homo sapiens	O60894 (AlphaFold model)
Calcitonin gene-related peptide 1	P	protein	38	Homo sapiens	P06881
Calcitonin receptor	R	protein	501	Homo sapiens	P30988

Sequence of entity 1 (A), FASTA

>9AUC_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>9AUC_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9AUC_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (N), FASTA

>9AUC_4 Nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Sequence of entity 5 (E), FASTA

>9AUC_5 Receptor activity-modifying protein 1 (chains E)
MKTIIALSYIFCLVFADYKDDDDKHGSCQEANYGALLRELCLTQFQVDMEAVGETLWCDW
GRTIRSYRELADCTWHMAEKLGCFWPNAEVDRFFLAVHGRYFRSCPISGRAVRDPPGSIL
YPFIVVPITVTLLVTALVVWQSKRTEGIV

Sequence of entity 6 (P), FASTA

>9AUC_6 Calcitonin gene-related peptide 1 (chains P)
ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFX

Sequence of entity 7 (R), FASTA

>9AUC_7 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
PLM	Palmitic acid	C16 H32 O2	5
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3
Y01	Cholesterol hemisuccinate	C31 H50 O4	2

Primary citation

Cryo-EM Structure of the Human Amylin 1 Receptor in Complex with CGRP and Gs Protein. Cao, J., Belousoff, M.J., Danev, R. et al. Biochemistry (2024) 63:1089-1096. DOI 10.1021/acs.biochem.4c00114 · PubMed

Other PDB entries of the same protein (UniProt P63092 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7BPH 1.57 Å, Crystal structure of GppNHp-bound GNAS in complex with the cyclic peptide inhibitor GN13
6AU6 1.7 Å, Crystal structure of GDP-bound human GNAS R201C mutant
8F0K 1.9 Å, Human Amylin3 Receptor in complex with Gs and Pramlintide analogue peptide San385
7E5E 1.95 Å, Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20
7MBX 1.95 Å, Human Cholecystokinin 1 receptor (CCK1R) Gs complex
8F0J 2.0 Å, Calcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
8F2B 2.0 Å, Amylin 3 Receptor in complex with Gs and Pramlintide analogue peptide San45
9XXT 2.0 Å, Cryo-EM structure of lysophosphatidylserine (18:0)-bound GPR174-Gs complex
6X18 2.1 Å, GLP-1 peptide hormone bound to Glucagon-Like peptide-1 (GLP-1) Receptor
6X19 2.1 Å, Non peptide agonist CHU-128, bound to Glucagon-Like peptide-1 (GLP-1) Receptor
9NTU 2.1 Å, Cryo-EM structure of BETP-GLP-1(9-36)-GLP-1R-Gs complex
7RTB 2.14 Å, Peptide-19 bound to the Glucagon-Like Peptide-1 Receptor (GLP-1R)

Browse structure collections

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