9C7F: Human monoclonal antibody MAD24-01

Human monoclonal antibody MAD24-01 bound to the N-terminus of cleaved circumsporozoite protein. Determined by X-ray diffraction at 1.82 Å resolution. Released 25 Dec 2024.

Method: X-ray diffraction
Resolution: 1.82 Å
Organisms: Homo sapiens, Plasmodium falciparum 3D7
Chains: 12
Atoms: 15,148
Mol. weight: 198.69 kDa
Released: 25 Dec 2024

Explore 9C7F in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9C7F contains 79 α-helices and 184 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	12
β-strand	11-12	2	13
β-strand	18-25	8	12
α-helix	29-31	3
β-strand	34-39	6	14
β-strand	45-51	7	14
β-strand	57-59	3	14
α-helix	61-63	3
β-strand	67-72	6	12
α-helix	73-75	3
β-strand	77-82	6	12
α-helix	84-86	3
β-strand	88-98	11	14
β-strand	100A-103	8	14
β-strand	107-109	3	14
β-strand	110-111	2	13
α-helix	114-116	3
β-strand	117	1	15
α-helix	118-119	2
β-strand	120-124	5	16
α-helix	125-127	3
β-strand	135-145	11	16
β-strand	146	1	15
β-strand	151-154	4	17
α-helix	155-157	3
β-strand	159	1	17
β-strand	163-165	3	16
α-helix	166-168	3
β-strand	169-170	2	16
β-strand	176-185	10	16
α-helix	186-188	3
β-strand	189	1	18
β-strand	192	1	18
β-strand	195-200	6	17
α-helix	201-203	3
β-strand	205-210	6	17

Chain B: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	19
β-strand	10-13	4	20
β-strand	19-25	7	19
β-strand	33-38	6	20
β-strand	45-49	5	20
β-strand	53-54	2	20
α-helix	55	1
β-strand	62-66	5	19
β-strand	70-75	6	19
α-helix	80-82	3
β-strand	85-90	6	20
α-helix	96	1
β-strand	97-98	2	20
β-strand	102-106	5	20
β-strand	111	1	21
α-helix	112-113	2
β-strand	114-118	5	22
α-helix	119-121	3
α-helix	122-126	5
β-strand	129-139	11	22
β-strand	140	1	21
β-strand	145-150	6	23
β-strand	153-154	2	23
α-helix	155	1
β-strand	159-163	5	22
α-helix	164-167	4
β-strand	173-182	10	22
α-helix	183-187	5
β-strand	191-198	8	23
β-strand	201-208	8	23

Chain C: 11 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	24
β-strand	11-12	2	25
β-strand	18-25	8	24
α-helix	29-31	3
β-strand	34-39	6	26
β-strand	45-51	7	26
β-strand	57-59	3	26
β-strand	67-72	6	24
α-helix	73-75	3
β-strand	77-82	6	24
α-helix	84-86	3
β-strand	88-98	11	26
β-strand	100A-103	8	26
β-strand	107-109	3	26
β-strand	110-111	2	25
α-helix	114-116	3
β-strand	117	1	27
α-helix	118-119	2
β-strand	120-124	5	28
α-helix	128-130	3
β-strand	131-132	2	28
β-strand	135-145	11	28
β-strand	146	1	27
β-strand	151-154	4	29
α-helix	155-157	3
β-strand	159	1	29
β-strand	163-165	3	28
α-helix	166-168	3
β-strand	169-170	2	28
β-strand	176-185	10	28
α-helix	186-188	3
β-strand	195-200	6	29
α-helix	201-203	3
β-strand	205-210	6	29
α-helix	212-214	3

Chain D: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	30
β-strand	10-13	4	31
β-strand	19-25	7	30
β-strand	33-38	6	31
β-strand	45-49	5	31
β-strand	53-54	2	31
α-helix	55	1
β-strand	62-67	6	30
β-strand	70-75	6	30
α-helix	80-82	3
β-strand	85-90	6	31
α-helix	96	1
β-strand	97-98	2	31
β-strand	102-106	5	31
β-strand	111	1	32
α-helix	112-113	2
β-strand	114-118	5	33
α-helix	119-121	3
α-helix	122-126	5
β-strand	129-139	11	33
β-strand	140	1	32
β-strand	145-150	6	34
β-strand	153-154	2	34
α-helix	155	1
β-strand	159-163	5	33
α-helix	164-167	4
β-strand	173-182	10	33
α-helix	183-186	4
β-strand	191-198	8	34
β-strand	201-208	8	34

Chain E: 10 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	35
β-strand	11-12	2	36
β-strand	18-25	8	35
α-helix	29-31	3
β-strand	34-39	6	37
β-strand	45-51	7	37
β-strand	57-59	3	37
β-strand	67-72	6	35
α-helix	73-75	3
β-strand	77-82	6	35
α-helix	84-86	3
β-strand	88-98	11	37
β-strand	100A-103	8	37
β-strand	107-109	3	37
β-strand	110-111	2	36
α-helix	114-116	3
β-strand	117	1	38
α-helix	118-119	2
β-strand	120-124	5	39
α-helix	128-130	3
β-strand	131-132	2	39
β-strand	135-145	11	39
β-strand	146	1	38
β-strand	151-154	4	40
α-helix	155-157	3
β-strand	159	1	40
β-strand	163-165	3	39
α-helix	166-168	3
β-strand	169-170	2	39
β-strand	176-185	10	39
α-helix	186-188	3
β-strand	195-200	6	40
α-helix	201-203	3
β-strand	205-210	6	40

Chain F: 9 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	41
β-strand	10-13	4	42
β-strand	19-25	7	41
β-strand	33-38	6	42
β-strand	45-49	5	42
β-strand	53-54	2	42
α-helix	55	1
β-strand	62-67	6	41
β-strand	70-75	6	41
α-helix	80-82	3
β-strand	85-90	6	42
α-helix	96	1
β-strand	97-98	2	42
β-strand	102-106	5	42
β-strand	111	1	43
α-helix	112-113	2
β-strand	114-118	5	44
α-helix	119-121	3
α-helix	122-125	4
β-strand	129-139	11	44
β-strand	140	1	43
β-strand	145-150	6	45
β-strand	153-154	2	45
α-helix	155	1
β-strand	159-163	5	44
α-helix	164-167	4
β-strand	173-182	10	44
α-helix	183-186	4
β-strand	191-198	8	45
β-strand	201-208	8	45

Chain G: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	13	1	14

Chain H: 11 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	1
β-strand	11-12	2	2
β-strand	18-25	8	1
α-helix	29-31	3
β-strand	34-39	6	3
β-strand	45-51	7	3
β-strand	57-59	3	3
α-helix	61-63	3
β-strand	67-72	6	1
α-helix	73-75	3
β-strand	77-82	6	1
α-helix	84-86	3
β-strand	88-98	11	3
β-strand	100A-103	8	3
β-strand	107-109	3	3
β-strand	110-111	2	2
α-helix	114-116	3
β-strand	117	1	4
α-helix	118-119	2
β-strand	120-124	5	5
α-helix	125-127	3
β-strand	135-145	11	5
β-strand	146	1	4
β-strand	151-154	4	6
α-helix	155-157	3
β-strand	159	1	6
β-strand	163-165	3	5
α-helix	166-168	3
β-strand	169-170	2	5
β-strand	176-185	10	5
α-helix	186-188	3
β-strand	195-200	6	6
α-helix	201-203	3
β-strand	205-210	6	6

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Monoclonal antibody MAD24-01 Fab Heavy Chain	A, C, E, H	protein	225	Homo sapiens
Monoclonal antibody MAD24-01 Fab Light Chain	B, D, F, L	protein	212	Homo sapiens
Circumsporozoite protein	G, I, J, Q	protein	18	Plasmodium falciparum 3D7	Q7K740 (AlphaFold model)

Sequence of entity 1 (A, C, E, H), FASTA

>9C7F_1 Monoclonal antibody MAD24-01 Fab Heavy Chain (chains A, C, E, H)
QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAPGKGLEWVSYIGSSNTYTSY
AASVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREIERSGWVRGNDYWGRGTLVTV
SSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQ
SSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC

Sequence of entity 2 (B, D, F, L), FASTA

>9C7F_2 Monoclonal antibody MAD24-01 Fab Light Chain (chains B, D, F, L)
EIVLTQSPATLSLSPGERATLSCRASQNVSTYLAWYQQKPGQAPRLLMYDASKRATGIPA
RFSGSGSGTEFTLTISSLEPEDFAVYYCQQRRNWPLTFGGGTKVEIKRTVAAPSVFIFPP
SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT
LSKADYEKHKVYACEVTQGTTSVTKSFNRGEC

Sequence of entity 3 (G, I, J, Q), FASTA

>9C7F_3 Circumsporozoite protein (chains G, I, J, Q)
QPADGNPDPNANPNVDPN

Primary citation

Protective antibodies target cryptic epitope unmasked by cleavage of malaria sporozoite protein. Dacon, C., Moskovitz, R., Swearingen, K. et al. Science (2025) 387:eadr0510-eadr0510. DOI 10.1126/science.adr0510 · PubMed

Other PDB entries of the same protein (UniProt Q7K740 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7SG5 1.4 Å, Structure of PfCSP peptide 21 with antibody CIS43_Var2
9C79 1.46 Å, Human monoclonal antibody MAD21-101 bound to the N-terminus of cleaved circumsporozoite…
9NKZ 1.48 Å, Crystal structure of Fab MAM01 in complex with NANP6 peptide from circumsporozoite protein
9NL0 1.54 Å, Crystal structure of Fab MAM01 in complex with NPNA3 peptide from circumsporozoite protein
7SG6 1.55 Å, Structure of PfCSP peptide 21 with antibody CIS43_Var10
6PBV 1.57 Å, Crystal structure of Fab668 complex
9NCY 1.63 Å, Fab1392 in complex with the C-terminal alpha-TSR domain of the P. falciparum…
3VDJ 1.7 Å, Crystal structure of circumsporozoite protein aTSR domain, R32 native form
9DST 1.74 Å, Crystal structure of Fab MS-1805 in complex with N-terminal junction peptide from…
9NI1 1.75 Å, Fab389 in complex with the C-terminal alpha-TSR domain of the P. falciparum…
7RXP 1.76 Å, Fab1512 in complex with the C-terminal alpha-TSR domain of P. falciparum
6BQB 1.77 Å, MGG4 Fab in complex with peptide

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