9CC5: Amylin-NHO-22 Binder

De novo design of high-affinity protein binders to bio active peptide Amylin. Determined by X-ray diffraction at 1.87 Å resolution. Released 14 May 2025.

Method: X-ray diffraction
Resolution: 1.87 Å
Organisms: synthetic construct, Homo sapiens
Chains: 2
Atoms: 1,332
Mol. weight: 19.07 kDa
Released: 14 May 2025

Explore 9CC5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9CC5 contains 6 α-helices and 6 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	1
α-helix	7-9	3
β-strand	10-12	3	1
α-helix	17-40	24
β-strand	44-47	4	1
α-helix	50-67	18
α-helix	73-98	26
β-strand	104-114	11	1
β-strand	117-124	8	1
α-helix	126-132	7

Chain B: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	140-156	17
β-strand	159-162	4	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Amylin-NHO-22 Binder	A	protein	133	synthetic construct
Islet amyloid polypeptide	B	protein	37	Homo sapiens	P10997 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>9CC5_1 Amylin-NHO-22 Binder (chains A)
SLIVAVASPVVVAAHSPEDEERAEKEAERLRRRFAEELRKKGFEVVELDEETDEELRRWL
TKAIREATQAPTQEEFNQAVAEAIEKALERIEEIARRRHPDREVAAVLTVAVVHDGEVIA
TIFASPRLREALK

Sequence of entity 2 (B), FASTA

>9CC5_2 Islet amyloid polypeptide (chains B)
KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY

Primary citation

Diffusing protein binders to intrinsically disordered proteins. Liu, C., Wu, K., Choi, H. et al. Nature (2025) 644:809-817. DOI 10.1038/s41586-025-09248-9 · PubMed

Other PDB entries of the same protein (UniProt P10997 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8YG2 1.25 Å, Crystal structure of amyloidogenic peptide Piv-NFGAIL-NH2 from Islet Amyloid Polypeptide
5KO0 1.4 Å, Human Islet Amyloid Polypeptide Segment 15-FLVHSSNNFGA-25 Determined by MicroED
3FPO 1.5 Å, HSSNNF segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3FTK 1.5 Å, NVGSNTY segment from Islet Amyloid Polypeptide (IAPP or Amylin), hydrated crystal form
3FTL 1.6 Å, NVGSNTY segment from Islet Amyloid Polypeptide (IAPP or Amylin), dehydrated crystal form
3FTR 1.61 Å, Structure of an amyloid forming peptide SSTNVG from IAPP (alternate polymorph)
3DG1 1.66 Å, Segment SSTNVG derived from IAPP
3G7W 1.75 Å, Islet Amyloid Polypeptide (IAPP or Amylin) Residues 1 to 22 fused to Maltose Binding…
3FTH 1.84 Å, NFLVHSS segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3FR1 1.85 Å, NFLVHS segment from Islet Amyloid Polypeptide (IAPP or Amylin)
3G7V 1.86 Å, Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein
5KNZ 1.9 Å, Human Islet Amyloid Polypeptide Segment 19-SGNNFGAILSS-29 with Early Onset S20G Mutation…

Browse structure collections

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